Antiglycolytic Activities of 5-Fluorouracil and Related Pyrimidines in Mouse Ascites Carcinoma Cells with Special Reference to Inorganic Phosphate and Glucose

Oncology ◽  
1969 ◽  
Vol 23 (1) ◽  
pp. 1-32 ◽  
Author(s):  
M.W. Woods ◽  
D. Burk
Keyword(s):  
Special Reference ◽  
Inorganic Phosphate ◽  
Carcinoma Cells ◽  
Mouse Ascites

Changes Induced in Ascites Tumor Cells by Ultraviolet Irradiation

1956 ◽  
Vol 184 (3) ◽  
pp. 465-468
Author(s):  
A. Canzanelli ◽  
R. Sossen ◽  
D. Rapport
Keyword(s):  
Tumor Cells ◽  
Optical Density ◽  
Ultraviolet Irradiation ◽  
Inorganic Phosphate ◽  
Acid Precipitation ◽  
High Polymer ◽  
Ascites Tumor ◽  
Free Medium ◽  
Mouse Ascites ◽  
Supernatant Solution

Suspensions of mouse ascites tumor cells were irradiated in the cold with ultraviolet light. Observations were made on the changes in optical density at 260 mµ, and in the amount of acid-insoluble, acid-soluble and inorganic phosphate, as well as protein, in the suspension, the cell-free medium, and in the supernatant solution after acid precipitation, for the purpose of detecting changes in the nucleic acids and their derivatives. The data show that the irradiation caused a marked increase in the permeability of some or all of the cells. Under the circumstances of the irradiation, there was no evidence of the breakdown of high polymer nucleotides to smaller fragments, contrary to the findings with pure solutions of such compounds. It was incidentally found that the ribonuclease activity of the tumor cells was reduced to an average of one-third its original value by irradiation under the conditions of the experiments.

Respiration and Glycolysis in X-Irradiated Ehrlich Mouse Ascites Carcinoma Cells

1963 ◽  
Vol 20 (4) ◽  
pp. 586 ◽  
Author(s):  
Elmon L. Coe ◽  
Eugene N. Garcia ◽  
Kenneth H. Ibsen ◽  
Ralph W. McKee
Keyword(s):  
Carcinoma Cells ◽  
Mouse Ascites

Ultrastructural Alterations in Hela Cells Induced by Spider Venom

1967 ◽  
Vol 25 ◽  
pp. 20-21
Author(s):  
Dale E. McClendon ◽  
Paul N. Morgan ◽  
Bernard L. Soloff
Keyword(s):  
Growth Medium ◽  
Mammalian Cells ◽  
Carcinoma Cells ◽  
Venom Protein ◽  
Sterile Saline ◽  
Carbon Coated ◽  
Available Information ◽  
Loxosceles Reclusa ◽  
Essential Growth ◽  
Solution Cultures

It has been observed that minute amounts of venom from the brown recluse spider, Loxosceles reclusa, are capable of producing cytotoxic changes in cultures of certain mammalian cells (Morgan and Felton, 1965). Since there is little available information concerning the effect of venoms on susceptible cells, we have attempted to characterize, at the electron microscope level, the cytotoxic changes produced by the venom of this spider.Cultures of human epithelial carcinoma cells, strain HeLa, were initiated on sterile, carbon coated coverslips contained in Leighton tubes. Each culture was seeded with approximately 1x105 cells contained in 1.5 ml of a modified Eagle's minimum essential growth medium prepared in Hank's balanced salt solution. Cultures were incubated at 36° C. for three days prior to the addition of venom. The venom was collected from female brown recluse spiders and diluted in sterile saline. Protein determinations on the venom-were made according to the spectrophotometric method of Waddell (1956). Approximately 10 μg venom protein per ml of fresh medium was added to each culture after discarding the old growth medium. Control cultures were treated similarly, except that no venom was added. All cultures were reincubated at 36° C.

Plasmalemma localisation of inorganic phosphate in B cells pancreas

1978 ◽  
Vol 36 (2) ◽  
pp. 528-529
Author(s):  
F. B. P. Wooding ◽  
K. Pedley ◽  
N. Freinkel ◽  
R. M. C. Dawson
Keyword(s):  
Electron Microscope ◽  
B Cells ◽  
B Cell ◽  
Pancreatic Islets ◽  
High Glucose ◽  
Lead Acetate ◽  
Inorganic Phosphate ◽  
Slight Modification ◽  
Uranyl Acetate ◽  
Lead Phosphate

Freinkel et al (1974) demonstrated that isolated perifused rat pancreatic islets reproduceably release up to 50% of their total inorganic phosphate when the concentration of glucose in the perifusion medium is raised.Using a slight modification of the Libanati and Tandler (1969) method for localising inorganic phosphate by fixation-precipitation with glutaraldehyde-lead acetate we can demonstrate there is a significant deposition of lead phosphate (identified by energy dispersive electron microscope microanalysis) at or on the plasmalemma of the B cell of the islets (Fig 1, 3). Islets after incubation in high glucose show very little precipitate at this or any other site (Fig 2). At higher magnification the precipitate seems to be intracellular (Fig 4) but since any use of osmium or uranyl acetate to increase membrane contrast removes the precipitate of lead phosphate it has not been possible to verify this as yet.

Morphologic differences between abdominal and inguinal testes in stallions

1987 ◽  
Vol 45 ◽  
pp. 820-821
Author(s):  
F. Al-Bagdadi ◽  
D. Hoyt ◽  
P. Karns ◽  
G. Martin ◽  
M. Memon ◽  
...  
Keyword(s):  
Carcinoma Cells ◽  
Genital System ◽  
Diagnosis And Prognosis ◽  
Male Genital System ◽  
Morphological Differences ◽  
Hormone Imbalance ◽  
Made In ◽  
Male Genital

The most frequently occuring abnormality of the male genital system in mammals is the failure of one or both testes to descend into the scrotum. The reasons for abdominal or inguinal retention of testes could be anatomic malformation, faulty development or hormone imbalance.Cryptorchidism has been associated with either greatly reduced or absent spermatogenesis (Kaueakami et al, 1984), and being a source of neoplasia. According to Stick (1980), germinal carcinoma cells have been believed to be the cause of teratomas in equine cryptorchid testicles. Neoplasia has been reported in descended testes of unilateral cryptorchid patients (Martin et al, 1981).No distinction has been made in relating the problem of cryptorchid testes to inguinal or abdominal retention. The purpose of this study is to record the morphological differences between inguinal and abdominal cryptorchid testes as an aid in diagnosis and prognosis.

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