Granulopoietic Progenitor Cells (CFU-C) in Patients with Acute Leukemia: A Comparison of Two Different Culture Techniques

1985 ◽  
Vol 8 (4) ◽  
pp. 214-216
Author(s):  
H. Gerhartz ◽  
U. Jehn
Keyword(s):  
Acute Leukemia ◽  
Progenitor Cells ◽  
Culture Techniques ◽  
Granulopoietic Progenitor Cells

scholarly journals A comparison of granulopoiesis in culture from blood and marrow cells of nonleukemic individuals and patients with acute leukemia

Blood ◽  
1976 ◽  
Vol 48 (2) ◽  
pp. 235-243
Author(s):  
K Tebbi ◽  
S Rubin ◽  
DH Cowan ◽  
EA McCulloch
Keyword(s):  
Acute Leukemia ◽  
Progenitor Cells ◽  
Peripheral Blood ◽  
Tritiated Thymidine ◽  
Sedimentation Velocity ◽  
Newly Diagnosed ◽  
Synthetic Phase ◽  
Granulopoietic Progenitor Cells ◽  
Marrow Cells

The objective of this study was to compare the concentration of committed granulopoietic progenitor cells (CFU-C) in marrow and blood. For individuals without leukemia, a highly significant correlation was observed between the concentration of CFU-C obtained from the two sites. However, CFU-C in blood had a slower sedimentation velocity than that reported for marrow and were found not to be in the DNA synthetic phase of the cycle using the tritiated thymidine suicide tehcnique. In patients with acute leukemia, no correlation was observed between concentrations of CFU-C in marrow and peripheral blood, regardless of whether the patients were newly diagnosed, in remission, or in relapse. We concluded that studies of the peripheral blood do not yield the same information in respect to granulopoietic progenitor cells as do studies of the marrow.

scholarly journals A comparison of granulopoiesis in culture from blood and marrow cells of nonleukemic individuals and patients with acute leukemia

Blood ◽  
1976 ◽  
Vol 48 (2) ◽  
pp. 235-243 ◽  
Author(s):  
K Tebbi ◽  
S Rubin ◽  
DH Cowan ◽  
EA McCulloch
Keyword(s):  
Acute Leukemia ◽  
Progenitor Cells ◽  
Peripheral Blood ◽  
Tritiated Thymidine ◽  
Sedimentation Velocity ◽  
Newly Diagnosed ◽  
Synthetic Phase ◽  
Granulopoietic Progenitor Cells ◽  
Marrow Cells

Abstract The objective of this study was to compare the concentration of committed granulopoietic progenitor cells (CFU-C) in marrow and blood. For individuals without leukemia, a highly significant correlation was observed between the concentration of CFU-C obtained from the two sites. However, CFU-C in blood had a slower sedimentation velocity than that reported for marrow and were found not to be in the DNA synthetic phase of the cycle using the tritiated thymidine suicide tehcnique. In patients with acute leukemia, no correlation was observed between concentrations of CFU-C in marrow and peripheral blood, regardless of whether the patients were newly diagnosed, in remission, or in relapse. We concluded that studies of the peripheral blood do not yield the same information in respect to granulopoietic progenitor cells as do studies of the marrow.

scholarly journals Intersections of lung progenitor cells, lung disease and lung cancer

2017 ◽  
Vol 26 (144) ◽  
pp. 170054 ◽  
Author(s):  
Carla F. Kim
Keyword(s):  
Lung Cancer ◽  
Progenitor Cells ◽  
Cell Biology ◽  
Progenitor Cell ◽  
Genetically Engineered ◽  
Response To Therapy ◽  
Culture Techniques ◽  
Genetically Engineered Mouse Models ◽  
Approaches To Study ◽  
Lung Progenitor

The use of stem cell biology approaches to study adult lung progenitor cells and lung cancer has brought a variety of new techniques to the field of lung biology and has elucidated new pathways that may be therapeutic targets in lung cancer. Recent results have begun to identify the ways in which different cell populations interact to regulate progenitor activity, and this has implications for the interventions that are possible in cancer and in a variety of lung diseases. Today's better understanding of the mechanisms that regulate lung progenitor cell self-renewal and differentiation, including understanding how multiple epigenetic factors affect lung injury repair, holds the promise for future better treatments for lung cancer and for optimising the response to therapy in lung cancer. Working between platforms in sophisticated organoid culture techniques, genetically engineered mouse models of injury and cancer, and human cell lines and specimens, lung progenitor cell studies can begin with basic biology, progress to translational research and finally lead to the beginnings of clinical trials.

scholarly journals Granulopoietic progenitors in suspension culture: a comparison of stimulatory cells and conditioned media

Blood ◽  
1975 ◽  
Vol 45 (6) ◽  
pp. 811-821 ◽  
Author(s):  
Y Niho ◽  
JE Till ◽  
EA McCulloch
Keyword(s):  
Suspension Culture ◽  
Progenitor Cells ◽  
Blood Cells ◽  
Colony Formation ◽  
Kinetic Studies ◽  
Suspension Cultures ◽  
Velocity Sedimentation ◽  
Peripheral Blood Cells ◽  
Functional Heterogeneity ◽  
Granulopoietic Progenitor Cells

Abstract Kinetic studies have been carried out to investigate the functional heterogeneity previously observed in populations of human marrow or peripheral blood cells separated by velocity sedimentation. The results obtained confirm the earlier results, in that slowly-sedimenting cells were found to stimulate both colony formation by granulopoietic progenitors and an increase in numbers of granulopoietic progenitors in suspension culture, while rapidly-sedimenting cells stimulated only colony formation and not increased progenitors in suspension cultures. Investigations of the properties of media conditioned by these two subpopulations of cells revealed no clear differences between them; both stimulated suspension cultures as well as colony formation, and both lost the former activity, but not the latter, after dialysis. The results contribute to the evidence that more than one process is regulated in cultures of granulopoietic progenitor cells.

scholarly journals Mouse acute leukemia develops independent of self-renewal and differentiation potentials in hematopoietic stem and progenitor cells

2019 ◽  
Vol 3 (3) ◽  
pp. 419-431 ◽  
Author(s):  
Fang Dong ◽  
Haitao Bai ◽  
Xiaofang Wang ◽  
Shanshan Zhang ◽  
Zhao Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Acute Leukemia ◽  
Progenitor Cells ◽  
Lymphoblastic Leukemia ◽  
The Self ◽  
Myelogenous Leukemia ◽  
Mouse Bone Marrow ◽  
Self Renewal ◽  
Hematopoietic Stem ◽  
Stem And Progenitor Cells

Abstract The cell of origin, defined as the normal cell in which the transformation event first occurs, is poorly identified in leukemia, despite its importance in understanding of leukemogenesis and improving leukemia therapy. Although hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) were used for leukemia models, whether their self-renewal and differentiation potentials influence the initiation and development of leukemia is largely unknown. In this study, the self-renewal and differentiation potentials in 2 distinct types of HSCs (HSC1 [CD150+CD41−CD34−Lineage−Sca-1+c-Kit+ cells] and HSC2 [CD150−CD41−CD34−Lineage−Sca-1+c-Kit+ cells]) and 3 distinct types of HPCs (HPC1 [CD150+CD41+CD34−Lineage−Sca-1+c-Kit+ cells], HPC2 [CD150+CD41+CD34+Lineage−Sca-1+c-Kit+ cells], and HPC3 [CD150−CD41−CD34+Lineage−Sca-1+c-Kit+ cells]) were isolated from adult mouse bone marrow, and examined by competitive repopulation assay. Then, cells from each population were retrovirally transduced to initiate MLL-AF9 acute myelogenous leukemia (AML) and the intracellular domain of NOTCH-1 T-cell acute lymphoblastic leukemia (T-ALL). AML and T-ALL similarly developed from all HSC and HPC populations, suggesting multiple cellular origins of leukemia. New leukemic stem cells (LSCs) were also identified in these AML and T-ALL models. Notably, switching between immunophenotypical immature and mature LSCs was observed, suggesting that heterogeneous LSCs play a role in the expansion and maintenance of leukemia. Based on this mouse model study, we propose that acute leukemia arises from multiple cells of origin independent of the self-renewal and differentiation potentials in hematopoietic stem and progenitor cells and is amplified by LSC switchover.

scholarly journals Neoplastic transformation of normal hematopoietic cells by constitutively activating mutations of c-kit receptor tyrosine kinase

Blood ◽  
1996 ◽  
Vol 88 (3) ◽  
pp. 995-1004 ◽  
Author(s):  
H Kitayama ◽  
T Tsujimura ◽  
I Matsumura ◽  
K Oritani ◽  
H Ikeda ◽  
...  
Keyword(s):  
Mast Cell ◽  
Acute Leukemia ◽  
Tyrosine Kinase ◽  
Progenitor Cells ◽  
Receptor Tyrosine Kinase ◽  
Bone Marrow Cells ◽  
Hematopoietic Cells ◽  
Hematologic Malignancies ◽  
Hematopoietic Progenitor Cells ◽  
Hematopoietic Progenitor

Abstract The c-kit proto-oncogene encodes a receptor tyrosine kinase that is crucial to hematopoiesis, melanogenesis, and gametogeneis. Although the enzymatic activity of the c-kit product (KIT) is regulated by its ligand, both the Val559-->Gly (G559) mutation in the juxtamembrane domain and the Asp814-->Val (V814) mutation in the phosphotransferase domain lead to constitutive activation of KIT. By retroviral infection of hematopoietic progenitor cells with KIT(G559) or KIT(V814), KIT(G559) induced development of granulocyte/macrophage and mast-cell colonies in vitro without the addition of exogenous growth factors. KIT(V814) induced factor-independent growth of various types of hematopoietic progenitor cells, resulting in the development of mixed erythroid/myeloid colonies in addition to granulocyte/macrophage and mast-cell colonies. Furthermore, transplantation of KIT(G559) and KIT(V814)-infected bone marrow cells led to development of acute leukemia in one of 10 and six of 10 transplanted mice, respectively. No mice developed hematologic malignancies after transplantation of wild- type KIT-infected cells. Furthermore, transgenic mice expressing KIT(V814) developed acute leukemia or malignant lymphoma. These results demonstrate a direct role of the mutant KITs, particularly KIT(V814), in tumorigenesis of hematopoietic cells and suggest that similar mutations may contribute to the development of human hematologic malignancies.

scholarly journals Good and poor CD34+ cells mobilization in acute leukemia: analysis of factors affecting the yield of progenitor cells

2004 ◽  
Vol 33 (11) ◽  
pp. 1083-1087 ◽  
Author(s):  
D Pastore ◽  
G Specchia ◽  
A Mestice ◽  
A Liso ◽  
A Pannunzio ◽  
...  
Keyword(s):  
Acute Leukemia ◽  
Progenitor Cells ◽  
Factors Affecting ◽  
Cd34 Cells

In vitro growth of erythropoietic progenitor cells in long-term remission of acute leukemia

1986 ◽  
Vol 4 (3) ◽  
pp. 186-191 ◽  
Author(s):  
C. Peschel ◽  
G. Konwalinka ◽  
D. Geissler ◽  
H. Braunsteiner
Keyword(s):  
Acute Leukemia ◽  
Progenitor Cells ◽  
In Vitro Growth
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