Ultrastructural Localization and Quantification of Extracellular Calcium Binding Sites in Mouse Vibrissa and Human Scalp Follicles

1993 ◽  
Vol 6 (4) ◽  
pp. 259-267
Author(s):  
Cynthia J. Mills ◽  
Allen E. Buhl ◽  
Roger G. Ulrich ◽  
Arthur R. Diani
Keyword(s):  
Binding Sites ◽  
Calcium Binding ◽  
Ultrastructural Localization ◽  
Extracellular Calcium ◽  
Calcium Binding Sites

scholarly journals Calcium Binding Sites on Human Blood Platelets

1977 ◽  
Author(s):  
S. Heptinstall
Keyword(s):  
Binding Sites ◽  
Calcium Binding ◽  
Blood Platelets ◽  
Human Platelets ◽  
Extracellular Calcium ◽  
Scatchard Analysis ◽  
Magnesium Ions ◽  
Binding Data ◽  
Calcium Binding Sites ◽  
Human Blood Platelets

Extracellular calcium ions are required for platelets to aggregate in response to various aggregating agents. Although magnesium ions can sometimes stimulate aggregation they only do so when a small amount of calcium is present. The calcium bound to washed human platelets suspended in buffered saline containing 0-200μM+5CaCl2 depends upon the extracellular calcium concentration. Scatchard analysis of the binding data suggests that a few (0,8 × 106) relatively high affinity (K = 85,000) calcium binding sites are present on each platelet. When 2.5mM MgCl2 is included in the saline suspensions the calcium bound to the platelets is only reduced at the higher calcium concentrations. Magnesium ions do not displace the tightly bound calcium. It is suggested that these specific calcium binding sites are involved in platelet aggregation.

Ultrastructural localization of calcium-binding sites in the electrocyte of Electrophorus electricus (L.)

1979 ◽  
Vol 38 (1) ◽  
pp. 97-104
Author(s):  
T.C. De Araujo Jorge ◽  
W. De Souza ◽  
R.D. Machado
Keyword(s):  
Plasma Membrane ◽  
Binding Sites ◽  
Calcium Binding ◽  
Synaptic Vesicles ◽  
Ultrastructural Localization ◽  
Synaptic Membrane ◽  
Electrophorus Electricus ◽  
Calcium Binding Sites

Calcium-binding sites were detected in the electrocyte of Electrophorus electricus (L.) using the Oschman & Wall technique, in which CaCl2 was added to the fixative and washing solutions. Deposits were seen scattered along the plasma membrane of the electrocyte, inside mitochondria, associated with the post-synaptic membrane and the membrane of synaptic vesicles.

Ultrastructural localization of calcium binding sites on human muscle cell surface

1989 ◽  
Vol 12 (11) ◽  
pp. 910-914 ◽  
Author(s):  
M. Moggio ◽  
S. Jann ◽  
L. Adobbati ◽  
A. Prelle ◽  
A. Gallanti ◽  
...  
Keyword(s):  
Cell Surface ◽  
Muscle Cell ◽  
Binding Sites ◽  
Calcium Binding ◽  
Ultrastructural Localization ◽  
Human Muscle ◽  
Calcium Binding Sites

scholarly journals Mutation of the high affinity calcium binding sites in cardiac troponin C.

1992 ◽  
Vol 267 (2) ◽  
pp. 825-831 ◽  
Author(s):  
J C Negele ◽  
D G Dotson ◽  
W Liu ◽  
H L Sweeney ◽  
J A Putkey
Keyword(s):  
Binding Sites ◽  
Calcium Binding ◽  
Cardiac Troponin ◽  
Troponin C ◽  
High Affinity ◽  
Cardiac Troponin C ◽  
Calcium Binding Sites

Rat GTP cyclohydrolase I is a homodecameric protein complex containing high-affinity calcium-binding sites 1 1Edited by W. Baumeister

1998 ◽  
Vol 279 (1) ◽  
pp. 189-199 ◽  
Author(s):  
Michel O Steinmetz ◽  
Christoph Plüss ◽  
Urs Christen ◽  
Bettina Wolpensinger ◽  
Ariel Lustig ◽  
...  
Keyword(s):  
Protein Complex ◽  
Binding Sites ◽  
Calcium Binding ◽  
Gtp Cyclohydrolase I ◽  
Gtp Cyclohydrolase ◽  
High Affinity ◽  
Calcium Binding Sites
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