Autoradiographic Study of the Effects of Psoralen and Ultraviolet Light on DNA, RNA and Protein Synthesis in Cultured Keratinocytes and Psoriatic Skin

1988 ◽  
Vol 1 (4) ◽  
pp. 250-258 ◽  
Author(s):  
B. Bertaux ◽  
G. Moreno ◽  
F. Vinzens ◽  
L. Dubertret
Keyword(s):  
Protein Synthesis ◽  
Ultraviolet Light ◽  
Autoradiographic Study ◽  
Psoriatic Skin ◽  
Cultured Keratinocytes ◽  
Rna And Protein Synthesis

Effect of Ultraviolet Light on RNA and Protein Synthesis in Differentiated Epidermal Cells

Nature ◽  
1967 ◽  
Vol 216 (5119) ◽  
pp. 1031-1032 ◽  
Author(s):  
KIMIE FUKUYAMA ◽  
WILLIAM L. EPSTEIN ◽  
JOHN H. EPSTEIN
Keyword(s):  
Protein Synthesis ◽  
Ultraviolet Light ◽  
Epidermal Cells ◽  
Rna And Protein Synthesis

Autoradiographic study of RNA and protein synthesis in sectioned peripheral nerves

1982 ◽  
Vol 76 (1) ◽  
pp. 72-82 ◽  
Author(s):  
C. Benech ◽  
J.R. Sotelo ◽  
J. Menéndez ◽  
R. Correa-Luna
Keyword(s):  
Protein Synthesis ◽  
Peripheral Nerves ◽  
Autoradiographic Study ◽  
Rna And Protein Synthesis

FEMALE ENDOCRINE CONTROL MECHANISMS DURING THE NEONATAL PERIOD

1972 ◽  
Vol 70 (2) ◽  
pp. 396-408 ◽  
Author(s):  
K.-D. Schulz ◽  
H. Haarmann ◽  
A. Harland
Keyword(s):  
Protein Synthesis ◽  
Reproductive System ◽  
Rna Synthesis ◽  
Neonatal Period ◽  
High Dose ◽  
Female Reproductive System ◽  
Endocrine Control ◽  
Hypothalamic Region ◽  
Rna And Protein Synthesis ◽  
Physiological Dose

ABSTRACT The present investigation deals with the oestrogen-sensitivity of the female reproductive system during the neonatal period. Newborn female guinea pigs were used as test animals. At different times after a single subcutaneous injection of a physiological dose of 0.1 μg or an unphysiologically high dose of 10 μg 17β-oestradiol/100 g body weight, the RNA- and protein-synthesis was examined in the hypothalamic region, pituitary, cerebral cortex, liver, adrenal gland, ovary and uterus. With a physiological dose an increase in organ weight, protein content, RNA-and protein-synthesis was found only in the uterus. These alterations turned out to be dose-dependent. In addition to the findings in the uterus an inhibition of the aminoacid incorporation rate occurred in the liver following the injection of the high oestradiol dose. As early as 1 hour after the administration of 0.1 μg 17β-oestradiol an almost 100% increase in uterine protein synthesis was detectable. This result demonstrates a high oestrogen-sensitivity of this organ during the neonatal period. All the other organs of the female reproductive system such as the hypothalamus, pituitary and ovary did not show any oestrogen response. Therefore the functional immaturity of the uterus during post partem life is not the result of a deficient hormone sensitivity but is correlated with the absence of a sufficient hormonal stimulus at this time. The investigation on the effects of actinomycin resulted in different reactions in the uterus and liver. In contrast to the liver a paradoxical actinomycin effect was found in the uterus after treatment with actinomycin alone. This effect is characterized by a small inhibition of RNA-synthesis and a 50% increase in protein synthesis. The treatment of the newborn test animals with actinomycin and 17β-oestradiol together abolished the oestrogen-induced stimulation of the uterine RNA-and protein-synthesis. Consequently, the effect of oestrogens during the neonatal period is also connected with the formation of new proteins via an increased DNA-directed RNA-synthesis.

A Method of Maintaining Parenchymal Cells from Adult Rat Liver In Vitro

1972 ◽  
Vol 11 (1) ◽  
pp. 249-260
Author(s):  
J. ALWEN ◽  
JENNIFER J. GALLHAI-ATCHARD
Keyword(s):  
Electron Microscopy ◽  
Endothelial Cells ◽  
Protein Synthesis ◽  
Light And Electron Microscopy ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Rna And Protein Synthesis ◽  
Parenchymal Cells

A method for preparing suspensions of adult rat hepatocytes suitable for maintenance in vitro is described. Cultures were established from the cell suspensions by the squash technique. Cells were examined by light and electron microscopy; histochemically for glycogen, bile, lipid and glucose-6-phosphatase; and by autoradiography for DNA, RNA and protein synthesis. Hepatocytes could be maintained in vitro for at least 3 days and began to aggregate after 1 day. Uridine and leucine were incorporated, but not thymidine. Cultures consisted mainly of hepatocytes, though reticulo-endothelial cells were sometimes present.

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