KRJ-I and BON Cell Lines: Defining an Appropriate Enterochromaffin Cell Neuroendocrine Tumor Model

2009 ◽  
Vol 89 (4) ◽  
pp. 458-470 ◽  
Author(s):  
Zakiya-Luna Siddique ◽  
Ignat Drozdov ◽  
Jared Floch ◽  
Bjorn I. Gustafsson ◽  
Kamilla Stunes ◽  
...  
Keyword(s):  
Neuroendocrine Tumor ◽  
Cell Lines ◽  
Tumor Model ◽  
Enterochromaffin Cell

scholarly journals In vitro3-dimensional tumor model for radiosensitivity of HPV positive OSCC cell lines

2015 ◽  
Vol 16 (8) ◽  
pp. 1231-1240 ◽  
Author(s):  
Mei Zhang ◽  
Barbara Rose ◽  
C Soon Lee ◽  
Angela M Hong
Keyword(s):  
Cell Lines ◽  
Tumor Model

Establishment of the First Well-differentiated Human Pancreatic Neuroendocrine Tumor Model

2018 ◽  
Vol 16 (3) ◽  
pp. 496-507 ◽  
Author(s):  
Daniel Benten ◽  
Yasmin Behrang ◽  
Ludmilla Unrau ◽  
Victoria Weissmann ◽  
Gerrit Wolters-Eisfeld ◽  
...  
Keyword(s):  
Neuroendocrine Tumor ◽  
Tumor Model ◽  
Pancreatic Neuroendocrine Tumor ◽  
Well Differentiated

„IN VITRO“ AND „IN VIVO“ ANTITUMOR ACTIVITY OF PLATINUM(II) COMPLEXES WITH MALONIC ACID ON BREAST AND COLORECTAL CARCINOMA CELL LINES

2021 ◽  
Author(s):  
Andjela Franich ◽  
◽  
Milica Dimitrijević Stojanović ◽  
Snežana Rajković ◽  
Marina Jovanović ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Colorectal Carcinoma ◽  
Cell Lines ◽  
Tumor Model ◽  
Carcinoma Cells ◽  
Spectroscopic Techniques ◽  
Murine Cell Line ◽  
In Vivo Antitumor Activity

Four Pt(II) complexes of the general formula [Pt(L)(5,6-epoxy-1,10-phen)], where L is anion of malonic (mal, Pt1), 2-methylmalonic (Me-mal, Pt2), 2,2-dimethylmalonic (Me2-mal, Pt3) or 1,1- cyclobutanedicarboxylic (CBDCA, Pt4) acid while 5,6-epoxy-1,10-phen is bidentately coordinated 5,6-epoxy-5,6-dihydro-1,10-phenanthroline were synthesized and characterized by elemental microanalysis, IR, UV-Vis and NMR (1H and 13C) spectroscopic techniques. In vitro anticancer activity of novel platinum(II) complexes have been investigated on human and murine cancer cell lines, as well as normal murine cell line by MTT assay. The obtained results indicate that studied platinum(II) complexes exhibited strong cytotoxic activity against murine breast carcinoma cells (4T1), human (HCT116) and murine (CT26) colorectal carcinoma cells. Complex Pt3 display stronger selectivity toward carcinoma cells in comparison to other tested platinum(II) complexes exhibiting beneficial antitumor activity mainly via the induction of apoptosis, as well as inhibition of cell proliferation and migration. Further study showed that Pt3 complex also carry significant in vivo antitumor activity in orthotopical 4T1 tumor model without detected liver, kidney, lung, and heart toxicity. All results imply that these novel platinum(II) complexes have a good anti-tumor effect on breast and colorectal cancer in vivo and in vitro and the affinity to become possible candidates for treatment in anticancer therapy.

scholarly journals Importance of Cullin4 Ubiquitin Ligase in Malignant Pleural Mesothelioma

Cancers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 3460
Author(s):  
Mayura Meerang ◽  
Jessica Kreienbühl ◽  
Vanessa Orlowski ◽  
Seraina L. C. Müller ◽  
Michaela B. Kirschner ◽  
...  
Keyword(s):  
Cell Lines ◽  
Malignant Pleural Mesothelioma ◽  
Ubiquitin Ligase ◽  
Tumor Model ◽  
Progression Free Survival ◽  
Neurofibromatosis Type ◽  
Resistant Cell ◽  
Pleural Mesothelioma ◽  
G2 Cell Cycle Arrest

Neurofibromatosis type 2 (NF2), the tumor suppressor frequently lost in malignant pleural mesothelioma (MPM), suppresses tumorigenesis in part by inhibiting the Cullin4 ubiquitin ligase (CUL4) complex in the nucleus. Here, we evaluated the importance of CUL4 in MPM progression and tested the efficacy of cullin inhibition by pevonedistat, a small molecule inhibiting cullin neddylation. CUL4 paralogs (CUL4A and CUL4B) were upregulated in MPM tumor specimens compared to nonmalignant pleural tissues. High gene and protein expressions of CUL4B was associated with a worse progression-free survival of MPM patients. Among 13 MPM cell lines tested, five (38%) were highly sensitive to pevonedistat (half maximal inhibitory concentration of cell survival IC50 < 0.5 µM). This remained true in a 3D spheroid culture. Pevonedistat treatment caused the accumulation of CDT1 and p21 in both sensitive and resistant cell lines. However, the treatment induced S/G2 cell cycle arrest and DNA rereplication predominantly in the sensitive cell lines. In an in vivo mouse model, the pevonedistat treatment significantly prolonged the survival of mice bearing both sensitive and resistant MPM tumors. Pevonedistat treatment reduced growth in sensitive tumors but increased apoptosis in resistant tumors. The mechanism in the resistant tumor model may be mediated by reduced macrophage infiltration, resulting from the suppression of macrophage chemotactic cytokines, C-C motif chemokine ligand 2 (CCL2), expression in tumor cells.

Neuroendocrine tumor targeting: Study of novel gallium-labeled somatostatin radiopeptides in a rat pancreatic tumor model

2002 ◽  
Vol 98 (6) ◽  
pp. 930-937 ◽  
Author(s):  
Sylvie Froidevaux ◽  
Alex N. Eberle ◽  
Martine Christe ◽  
Lazar Sumanovski ◽  
Axel Heppeler ◽  
...  
Keyword(s):  
Neuroendocrine Tumor ◽  
Tumor Targeting ◽  
Pancreatic Tumor ◽  
Tumor Model

A novel neural stem cell-derived immunocompetent mouse model of glioblastoma for preclinical studies

2020 ◽  
Author(s):  
Barbara Costa ◽  
Michael Fletcher ◽  
Pavle Boskovic ◽  
Ekaterina L. Ivanova ◽  
Tanja Eisemann ◽  
...  
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Cell Lines ◽  
Tumor Model ◽  
Myeloid Cell ◽  
Molecular Heterogeneity ◽  
Double Knockout ◽  
Human Glioblastoma ◽  
Novel Treatments

AbstractGlioblastomas are the most lethal tumors affecting the central nervous system in adults. Simple and inexpensive syngeneic in vivo models that closely mirror human glioblastoma, including interactions between tumor and immune cells, are urgently needed for deciphering glioma biology and developing more effective treatments. Here, we generated glioblastoma cell lines by repeated in-vivo passaging of cells isolated from a neural stem cell-specific Pten/p53 double-knockout genetic mouse brain tumor model. Transcriptome and genome analyses of the cell lines revealed molecular heterogeneity comparable to that observed in human glioblastoma. Upon orthotopic transplantation into syngeneic hosts they formed high-grade gliomas that faithfully recapitulated the histopathological features, invasiveness and myeloid cell infiltration characteristic of human glioblastoma. These features make our cell lines unique and useful tools to study multiple aspects of glioblastoma pathomechanism and test novel treatments, especially immunotherapies in syngeneic preclinical models.

scholarly journals Can DCE-MRI Explain the Heterogeneity in Radiopeptide Uptake Imaged by SPECT in a Pancreatic Neuroendocrine Tumor Model?

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e77076 ◽  
Author(s):  
Karin Bol ◽  
Joost C. Haeck ◽  
Harald C. Groen ◽  
Wiro J. Niessen ◽  
Monique R. Bernsen ◽  
...  
Keyword(s):  
Neuroendocrine Tumor ◽  
Tumor Model ◽  
Pancreatic Neuroendocrine Tumor ◽  
Dce Mri

scholarly journals A water soluble tri-cationic porphyrin–EDTA conjugate induces apoptosis in human neuroendocrine tumor cell lines

2012 ◽  
Vol 40 ◽  
pp. 108-113 ◽  
Author(s):  
Gert Schwach ◽  
Patchanita Thamyongkit ◽  
Lorenz Michael Reith ◽  
Bernhard Svejda ◽  
Günther Knör ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Neuroendocrine Tumor ◽  
Cell Lines ◽  
Water Soluble ◽  
Tumor Cell Lines ◽  
Cationic Porphyrin

scholarly journals Ad-VT enhances the sensitivity of chemotherapy-resistant lung adenocarcinoma cells to gemcitabine and paclitaxel in vitro and in vivo

2022 ◽  
Author(s):  
Gaojie Song ◽  
Chao Shang ◽  
Lili Sun ◽  
Yiquan Li ◽  
Yilong Zhu ◽  
...  
Keyword(s):  
Cell Lines ◽  
A549 Cells ◽  
Resistance Index ◽  
Tumor Model ◽  
Resistant Cell ◽  
Drug Resistant ◽  
Chemotherapeutic Drugs ◽  
Drug Resistant Cell

SummaryBackground One of the main challenges in the clinical treatment of lung cancer is resistance to chemotherapeutic drugs. P-glycoprotein (P-gp)-mediated drug resistance is the main obstacle to successfully implementing microtubule-targeted tumor chemotherapy. Purpose In this study, we explored the effect of Ad-hTERTp-E1a-Apoptin (Ad-VT) on drug-resistant cell lines and the molecular mechanism by which Ad-VT combined with chemotherapy affects drug-resistant cells and parental cells. Methods In vitro, cell proliferation, colony formation, resistance index (RI), apoptosis and autophagy assays were performed. Protein expression was analyzed by Western blotting. Finally, a xenograft tumor model in nude mice was used to detect tumor growth and evaluate histological characteristics. Results Our results showed that Ad-VT had an obvious killing effect on A549, A549/GEM and A549/Paclitaxel cancer cells, and the sensitivity of drug-resistant cell lines to Ad-VT was significantly higher than that of parental A549 cells. Compared with A549 cells, A549/GEM and A549/Paclitaxel cells had higher autophagy levels and higher viral replication ability. Ad-VT decreased the levels of p-PI3k, p-Akt and p-mTOR and the expression of P-gp. In vivo, Ad-VT combined with chemotherapy can effectively inhibit the growth of chemotherapy-resistant tumors and prolong the survival of mice. Conclusions Thus, the combination of Ad-VT and chemotherapeutic drugs will be a promising strategy to overcome chemoresistance.

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