Characterization of B-Lymphocytes

2008 ◽  
pp. 211-229
Author(s):  
Michael Schlesier ◽  
Klaus Warnatz
Keyword(s):  
B Lymphocytes

Regulation of human B lymphocytes differentiation: Characterization of B cell stimulatory factors

1985 ◽  
pp. 89-100
Author(s):  
T. Hirano ◽  
H. Kikutani ◽  
K. Shimizu ◽  
H. Kishi ◽  
T. Taga ◽  
...  
Keyword(s):  
B Cell ◽  
B Lymphocytes

scholarly journals Characterization of the phytohemagglutinin-induced proliferating lymphocyte subpopulations in chronic lymphocytic leukemia patients using a clonogenic agar technique and monoclonal antibodies

Blood ◽  
1981 ◽  
Vol 58 (5) ◽  
pp. 1053-1055 ◽  
Author(s):  
S Davis
Keyword(s):  
T Cells ◽  
Monoclonal Antibodies ◽  
Chronic Lymphocytic Leukemia ◽  
B Lymphocytes ◽  
Peripheral Blood Lymphocytes ◽  
Lymphocytic Leukemia ◽  
Helper T Cells ◽  
Suppressor T Lymphocytes ◽  
Pha Stimulation

Abstract Peripheral blood lymphocytes from normal donors and patients with chronic lymphocytic leukemia, B-cell type, were purified into T, helper T, and suppressor T lymphocytes by fluorescence-activated cell sorting using OKT3, OKT4, and OKT8 monoclonal antibodies. The maximum response of the purified subpopulations to stimulation by phytohemagglutinin (PHA) was determined by measuring the production of colonies when the stimulated cells were grown on agar. The helper T cells in normal and CLL patients were the most responsive to PHA stimulation, although the responsiveness of helper T cells to PHA was decreased in CLL. Purified CLL B cells responded minimally to PHA stimulation, but normal B lymphocytes did not. The abnormal response of CLL lymphocytes to PHA appears to be due abnormal helper T cells, and, to a smaller extent, to the ability of CLL B lymphocytes to respond.

scholarly journals Characterization of the human CD5 endogenous retrovirus-E in B lymphocytes

2005 ◽  
Vol 6 (8) ◽  
pp. 663-671 ◽  
Author(s):  
Y Renaudineau ◽  
S Vallet ◽  
C Le Dantec ◽  
S Hillion ◽  
A Saraux ◽  
...  
Keyword(s):  
B Lymphocytes ◽  
Endogenous Retrovirus

Characterization of Pgp-1 Antigen on Murine B Lymphocytes Using a New Anti-Pgp-1 Monoclonal Antibody

Hybridoma ◽  
1991 ◽  
Vol 10 (2) ◽  
pp. 281-284 ◽  
Author(s):  
MANGALA RAO ◽  
ROBERT KNOX ◽  
DANIEL H. CONRAD
Keyword(s):  
Monoclonal Antibody ◽  
B Lymphocytes

scholarly journals Polyclonal activation of human B lymphocytes by Fc fragments. I. Characterization of the cellular requirements for Fc fragment-mediated polyclonal antibody secretion by human peripheral blood B lymphocytes.

1981 ◽  
Vol 154 (3) ◽  
pp. 778-790 ◽  
Author(s):  
E L Morgan ◽  
W O Weigle
Keyword(s):  
T Cells ◽  
B Lymphocytes ◽  
Polyclonal Antibody ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Cell Lineage ◽  
Fc Fragment ◽  
Antibody Secretion

Fc fragments derived from human immunoglobulin were found to be capable of inducing both a proliferative and polyclonal antibody response in human peripheral blood lymphocyte cultures. The cell population proliferating in response to Fc fragments belongs to the B cell lineage. Expression of polyclonal antibody formation requires the presence of both adherent monocytes and T cells. The role of the monocyte is to enzymatically cleave the Fc fragment into 19,000 mol wt Fc subfragments that are then able to induce polyclonal antibody secretion. Stimulation of polyclonal antibody production by Fc subfragments occurs in the absence of adherent monocytes but still requires the presence of T cells.

scholarly journals Antigen-initiated B-lymphocyte differentiation. VIII. Sedimentation velocity and buoyant density characterization of virgin antibody-forming cell progenitors in the adoptive immune response of unprimed CBA mice to 4-hydroxy-3-iodo-5-nitrophenylacetic acid-polymerized bacterial flagellin antigen.

1976 ◽  
Vol 143 (5) ◽  
pp. 1220-1238 ◽  
Author(s):  
J M Fidler ◽  
M C Howard ◽  
K Shortman
Keyword(s):  
B Cells ◽  
Physical Properties ◽  
B Cell ◽  
B Lymphocytes ◽  
Buoyant Density ◽  
Specific Antigen ◽  
Significant Proportion ◽  
Sedimentation Velocity ◽  
Cba Mice

The characteristics of antibody-forming cell (AFC) progenitors lacking previous contact with specific antigen (virgin AFC progenitors) has been studied using sedimentation velocity and buoyant density separation for the investigation of physically distinct B-cell subpopulations. Functional characterization of isolated subsets was made using a quantitative adoptive immune assay for the IgM AFC progenitors responding to the antigen 4-hydroxy-3-iodo-5-nitrophenylacetic acid conjugated polymerized bacterial flagellin. Extensive heterogeneity is present among B lymphocytes, only some subpopulations of which exhibit AFC progenitor function. In the spleen of adult conventional CBA mice, atypically fast sedimenting cells of low buoyant density are active, while typical small B lymphocytes do not appear to be progenitors of IgM AFC. Spleen of adult specific pathogen-free (SPF), germfree, and athymic nude mice give similar results, although a minor population of typical slowly sedimenting dense cells are active in the latter two sources. Adult conventional bone marrow cells are as physically and functionally heterogeneous as splenic B cells, and although a significant proportion of AFC progenitor activity is found among dense, slowly sedimenting cells, most of the activity is among low density, faster sedimenting cells. In contrast to this situation in adult animals, where most of the unprimed AFC progenitors are large, atypical B cells, the spleens of neonatal mice provide a site where virgin AFC progenitors with the physical properties of typical small B lymphocytes are found. While being present in conventional and SPF neonatal spleens, these virgin cells are predominant in 7-day-old germfree mouse spleen. These findings suggest that the newborn virgin B cell is a typical small lymphocyte. However, few cells of this type are found in the adult animal. The unprimed AFC-progenitor population in the adult consists of large, fast sedimenting, low buoyant density, adherent cells, the physical properties of which are characteristic of activated B lymphocytes. It is suggested that these atypical cells are derived from the small newborn virgin B cell by the nonspecific effects of environmental antigenic stimuli.

Molecular characterization of CD9 and CD63, two tetraspanin family members expressed in trout B lymphocytes

2015 ◽  
Vol 51 (1) ◽  
pp. 116-125 ◽  
Author(s):  
Rosario Castro ◽  
Beatriz Abós ◽  
Lucia González ◽  
Carolina Aquilino ◽  
Jaime Pignatelli ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
B Lymphocytes ◽  
Family Members ◽  
Tetraspanin Family

Demonstration and partial characterization of the interferon-gamma receptor on human B lymphocytes

1989 ◽  
Vol 40 (4) ◽  
pp. 417-430 ◽  
Author(s):  
Toshimasa Nakagawa ◽  
Naoko Nakagawa ◽  
Gita A. Delsing ◽  
David Volkman ◽  
John H. Kehrl
Keyword(s):  
B Lymphocytes ◽  
Interferon Gamma ◽  
Partial Characterization ◽  
Gamma Receptor
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