In-vitro Study of DNA-Synthesis Time and Cell-Cycle Time in Erythrocyte Precursors of Normal and Thalassaemic Subjects, Using a 3H- and 14C-Thymidine Double Labelling Technique

1967 ◽  
Vol 38 (3) ◽  
pp. 170-177 ◽  
Author(s):  
M. Kesse-Elias ◽  
E.B. Harriss ◽  
E. Gyftaki
Keyword(s):  
Cell Cycle ◽  
Dna Synthesis ◽  
Cycle Time ◽  
In Vitro Study ◽  
Vitro Study ◽  
Double Labelling ◽  
Cell Cycle Time ◽  
Synthesis Time ◽  
Labelling Technique

CHANGES IN CELL PROLIFERATION RATE IN MOUSE UTERINE EPITHELIUM DURING CONTINUOUS OESTROGEN TREATMENT

1974 ◽  
Vol 61 (1) ◽  
pp. 117-121 ◽  
Author(s):  
AUDREY E. LEE ◽  
L. A. ROGERS ◽  
GAIL TRINDER
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Dna Synthesis ◽  
Mitotic Index ◽  
Cycle Time ◽  
Luminal Epithelium ◽  
Cell Cycle Time ◽  
Oestrogen Treatment ◽  
Probability Of Transition ◽  
The Mean

SUMMARY Fraction of labelled mitoses (FLM) curves were constructed for mouse uterine luminal epithelium during oestradiol treatment; on day 2 when mitosis was high, and on days 4 and 9 when mitosis was low. No difference was found between the duration of DNA synthesis on these 3 days. The distance between the first and second peaks, usually taken as an estimate of the mean cell cycle time, did not change significantly between days 2 and 4, although the labelling index fell from 38 to 8%. The second peaks of the FLM curves became progressively lower on the three days examined, which was consistent with the interpretation that there was a reduction in the probability of transition of cells from G1 (the post-mitotic period) into the replicative phase of the cell cycle, resulting in the observed fall in mitotic index.

scholarly journals The effect of sinefungin on the macromolecular biosynthesis of Leishmania species

2018 ◽  
Vol 49 (2) ◽  
pp. 137
Author(s):  
A. TZORA (Α. ΤΖΩΡΑ) ◽  
F. LAWRENCE ◽  
M. ROBERT-GERO
Keyword(s):  
Dna Synthesis ◽  
Mode Of Action ◽  
Rna Synthesis ◽  
In Vitro Study ◽  
Leishmania Species ◽  
Sensitive Strain ◽  
Vitro Study ◽  
No Inhibition ◽  
Main Target

The aim of the work presented here was the in vitro study of the action of the antibiotic sinefungin on the macromolecules DNA, RNA and the proteins of different isolates of Leismannia spp. in relation to the resistance or to the sensitivity of the strains examined. The mode of action of sinefungin of the Leishmania strains was detected by incorporating radiolabeled thymidine, uridine and leucine into promastigote forms, to study the synthesis of DNA, RNA and proteins respectively. In the sensitive strain L. tropica a statistically significant reduction of the rate of incorporation of thymidine to the DNA of the parasite, resulted to a 64% inhibition of leishmanial DNA synthesis by sinefungin at a concentration of 2.6 mM, when the time of action of the drug was 24 hours. In the sensitive strain L. tropica the reduction of the rate of incorporation of thymidine was depended on the concentration and the time of action of the drug. No inhibition of leishmanial RNA synthesis was observed. Our research showed that the action of the antibiotic sinefungin to the macromolecular biosynthesis of the protozoan Leishmania is independent of the strain and is strongly related with the expression of the sensitivity and the resistance of the strain. The main target of the antibiotic examined was the DNA of the Leishmania strains sensitive to the drug.

DETERMINATION OF CELL CYCLE IN PLANTS USING BrdU-FPG

1980 ◽  
Vol 22 (2) ◽  
pp. 305-308 ◽  
Author(s):  
Kirby J. Evans ◽  
W. Gary Filion
Keyword(s):  
Cell Cycle ◽  
Dna Synthesis ◽  
Cycle Time ◽  
Differential Staining ◽  
Root Tip ◽  
Cell Cycle Time ◽  
Sister Chromatids ◽  
Vicia Faba L ◽  
Established Cell

The BrdU-FPG method for differential staining of sister chromatids was employed to estimate the cell cycle time in two plants species. The protocol is based upon the ability to differentiate cytologically between chromosomes which have completed two or three DNA synthesis phases. Vicia faba L. which has a well established cell cycle time was used to verify the technique. Utilizing this method the cell cycle time of Zebrina pendula Schnizl. root tip meristem cells was determined to be 17.0 hours.

The Role of Activin A in The Human Osteoblast Cell Cycle: A Preliminary Experimental in Vitro Study

2010 ◽  
Vol 118 (10) ◽  
pp. 708-712 ◽  
Author(s):  
N. Rosenberg ◽  
M. Soudry ◽  
O. Rosenberg ◽  
I. Blumenfeld ◽  
Z. Blumenfeld
Keyword(s):  
Cell Cycle ◽  
In Vitro Study ◽  
Activin A ◽  
Vitro Study ◽  
Human Osteoblast ◽  
Osteoblast Cell ◽  
Human Osteoblast Cell

scholarly journals Glyphosate affects methylation in the promoter regions of selected tumor suppressors as well as expression of major cell cycle and apoptosis drivers in PBMCs (in vitro study)

2020 ◽  
Vol 63 ◽  
pp. 104736 ◽  
Author(s):  
Ewelina Woźniak ◽  
Edyta Reszka ◽  
Ewa Jabłońska ◽  
Aneta Balcerczyk ◽  
Marlena Broncel ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Tumor Suppressors ◽  
In Vitro Study ◽  
Vitro Study ◽  
Promoter Regions
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