The Effect of Cytosine Arabinoside on Nucleic Acid Synthesis in Normal and Leukaemic Human Leucocytes in vitro

1969 ◽  
Vol 41 (6) ◽  
pp. 321-327 ◽  
Author(s):  
B.W.B. Chan
Keyword(s):  
Nucleic Acid ◽  
Cytosine Arabinoside ◽  
Acid Synthesis ◽  
Nucleic Acid Synthesis

scholarly journals 8-AZAGUANINE RESISTANCE IN MAMMALIAN CELLS I. HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE

Genetics ◽  
1972 ◽  
Vol 72 (2) ◽  
pp. 239-252 ◽  
Author(s):  
F D Gillin ◽  
D J Roufa ◽  
A L Beaudet ◽  
C T Caskey
Keyword(s):  
Nucleic Acid ◽  
Mammalian Cells ◽  
Chinese Hamster ◽  
Acid Synthesis ◽  
Ethyl Methanesulfonate ◽  
Nucleic Acid Synthesis ◽  
Wild Type ◽  
Chinese Hamster Cells ◽  
Hypoxanthine Guanine Phosphoribosyltransferase

ABSTRACT Chinese hamster cells were treated with ethyl methanesulfonate or N-methyl-N'-nitro-N-nitrosoguanidine, and mutants resistant to 8-azaguanine were selected and characterized. Hypoxanthine-guanine phosphoribosyltransferase activity of sixteen mutants is extremely negative, making them suitable for reversion to HGPRTase+. Ten of the extremely negative mutants revert at a frequency higher than 10-7 suggesting their point mutational character. The remaining mutants have demonstrable HGPRTase activity and are not useful for reversion analysis. Five of these mutants have < 2% HGPRTase and are presumably also HGPRTase point mutants. The remaining 14 mutants utilize exogenous hypoxanthine for nucleic acid synthesis poorly, and possess 20-150% of wild-type HGPRTase activity in in vitro. Their mechanism of 8-azaguanine resistance is not yet defined.

Fuel-mediated teratogenesis: biochemical effects of hypoglycemia during neurulation in mouse embryos in vitro

1989 ◽  
Vol 257 (2) ◽  
pp. E269-E276 ◽  
Author(s):  
E. S. Hunter ◽  
T. W. Sadler
Keyword(s):  
Nucleic Acid ◽  
Glucose Metabolism ◽  
Severe Hypoglycemia ◽  
Acid Synthesis ◽  
Mouse Embryos ◽  
Nucleic Acid Synthesis ◽  
Glycolytic Metabolism ◽  
Whole Embryo Culture ◽  
Altered Glucose

Hypoglycemia has been reported to induce congenital malformations and growth retardation in rodent embryos during the period of neural tube closure in vitro. However, the biochemical alterations responsible for the production of the dysmorphogenic effects have not been evaluated. Therefore, the rates of glucose metabolism by glycolysis, citric acid cycle, oxidative pentose phosphate pathway (PPP), and anabolic utilization were evaluated in mouse embryos and extraembryonic membranes using the whole embryo culture technique. Altered glucose metabolism by glycolysis and oxidative PPP, as well as altered anabolic synthesis, were produced by exposure to hypoglycemia. In embryos exposed to mild hypoglycemia (80 mg/dl) altered metabolism by the PPP and an associated effect on nucleic acid synthesis were in part responsible for the dysmorphogenic effects of this treatment. In contrast, severe hypoglycemia (40 mg/dl) appeared to have an immediate effect on glycolytic metabolism in addition to effects on the PPP and nucleic acid synthesis. Therefore, a multifactorial biochemical mechanism contributes to the induction of malformations by severe hypoglycemia in mouse embryos in vitro. Furthermore, the differential effects of moderate vs. severe hypoglycemia on glycolytic metabolism, and possibly energy production, may account for the differences in the severity of these treatments on embryonic growth and the incidence of malformations.

Pineal effects upon pituitary protein and nucleic acid synthesis in vitro

1976 ◽  
Vol 2 (4) ◽  
pp. 207-210
Author(s):  
Nancy S. Peress ◽  
Gollapudi G. Murthy ◽  
Robert J. Balcom
Keyword(s):  
Nucleic Acid ◽  
Acid Synthesis ◽  
Nucleic Acid Synthesis

Action of Daunomycin on Nucleic Acid Synthesis in Hepatoma Ascites Cells Incubated « in vitro »

1964 ◽  
Vol 50 (4) ◽  
pp. 261-266 ◽  
Author(s):  
Aldo Rusconi ◽  
Emidio Calendi
Keyword(s):  
Nucleic Acid ◽  
High Activity ◽  
Cell Population ◽  
Acid Synthesis ◽  
Nucleic Acid Synthesis ◽  
Experimental Conditions ◽  
Partial Inhibition ◽  
Higher Doses

The action of Dannomycin, an antibiotic of Rhodomycin group, was studied following the incorporation of Adenine-8-14C into nucleic acid of hepatoma ascites cells incubated « in vitro ». Doses of 20 μg/ml of the antibiotic were able to inhibit the uptake of the precursor by RNA; the activity of the drug appeared to be dependent on the density of the cell population. As it was noted that, under our experimental conditions, a partial inhibition of the incorporation was caused by these doses of Daunomycin, some RNA fractions were isolated from cells and tested for their sensitivity to the antibiotic. A high-activity RNA extracted from nuclei appeared to be the most sensitive to the Daunomycin action. At higher doses of Daunomycin the incorporation into DNA was also lowered.

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