A Simplified One-Step Procedure for the Simultaneous Determination of Complement Receptor Lymphocytes and Lymphocytes with Membrane-Bound Immunoglobulins

1977 ◽  
Vol 57 (2) ◽  
pp. 74-80 ◽  
Author(s):  
H. Winterleitner ◽  
M. Rotter ◽  
W. Knapp
Keyword(s):  
Simultaneous Determination ◽  
Complement Receptor ◽  
Step Procedure ◽  
One Step ◽  
Membrane Bound

scholarly journals Isocratic High-Performance Liquid Chromatographic Method with Ultraviolet Detection for Simultaneous Determination of Levels of Voriconazole and Itraconazole and Its Hydroxy Metabolite in Human Serum

2005 ◽  
Vol 49 (8) ◽  
pp. 3569-3571 ◽  
Author(s):  
GholamAli Khoschsorur ◽  
Franz Fruehwirth ◽  
Sieglinde Zelzer
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Specific Method ◽  
Minimum Detectable Concentration ◽  
Detectable Concentration ◽  
One Step ◽  
Liquid Chromatographic

ABSTRACT A simple, specific method is presented for simultaneous determination of voriconazole and itraconazole and its metabolite, hydroxyitraconazole, in human serum using one-step liquid-liquid extraction and high-performance liquid chromatography. Linearity tests ranged from 0.1 to 8.0 μg/ml; the minimum detectable concentration was 0.03 μg/ml.

scholarly journals A simple one-step procedure for the separation of calpain I, calpain II and calpastatin

1985 ◽  
Vol 231 (1) ◽  
pp. 201-204 ◽  
Author(s):  
J O Karlsson ◽  
S Gustavsson ◽  
C Hall ◽  
E Nilsson
Keyword(s):  
Salt Concentration ◽  
Soluble Fraction ◽  
Rabbit Brain ◽  
Endogenous Inhibitor ◽  
Linear Gradient ◽  
Step Procedure ◽  
Neuronal Proteins ◽  
One Step ◽  
Membrane Bound ◽  
Calpain Ii

The soluble fraction from rabbit brain was adsorbed on a column of phenyl-Sepharose. By applying a linear gradient with decreasing salt concentration and increasing pH, it was possible to separate calpain I and calpain II from each other and from the endogenous inhibitor calpastatin. Both enzymes were capable of degrading endogenously labelled neuronal proteins, including slowly axonally transported soluble proteins and rapidly transported membrane-bound proteins, as well as casein.

Improved rapid assay of uric acid in serum by liquid chromatography.

1988 ◽  
Vol 34 (12) ◽  
pp. 2567-2568 ◽  
Author(s):  
M Tanaka ◽  
M Hama
Keyword(s):  
Uric Acid ◽  
High Performance ◽  
Rapid Determination ◽  
Correlation Coefficients ◽  
Gel Permeation Chromatography ◽  
Serum Samples ◽  
Step Procedure ◽  
One Step ◽  
Highly Porous

Abstract This improved method for rapid determination of uric acid in serum is based on high-performance liquid-gel-permeation chromatography, with hydrophilic and highly porous vinyl alcohol copolymer as packing material. It has the following advantages: no need for sample deproteinization or use of a precolumn, more than 500 serum samples can be analyzed without having to regenerate or recondition the analytical apparatus, and the analysis for uric acid is a one-step procedure. Correlation coefficients between this method and other methods are very good (r = 0.998, 0.999).

MOF/PAN nanofiber-derived N-doped porous carbon materials with excellent electrochemical activity for the simultaneous determination of catechol and hydroquinone

2019 ◽  
Vol 43 (9) ◽  
pp. 3913-3920 ◽  
Author(s):  
Mengxin Zhang ◽  
Meishan Li ◽  
Wangui Wu ◽  
Junkun Chen ◽  
Xiuling Ma ◽  
...  
Keyword(s):  
Electrochemical Sensor ◽  
Simultaneous Determination ◽  
Porous Carbon ◽  
Carbon Materials ◽  
Electrochemical Activity ◽  
Porous Carbon Materials ◽  
One Step ◽  
Pan Nanofiber

In this work, the obtained C-ZIF-67/PAN via one-step pyrolysis of hybrid ZIF-67/PAN nanofibers were used to fabricate electrochemical sensor for the determination of benzenediol isomer.

A Photometric Assay for Blood Coagulation Factor XIII

1991 ◽  
Vol 65 (05) ◽  
pp. 535-540 ◽  
Author(s):  
Karl Fickenscher ◽  
Angela Aab ◽  
Werner Stüber
Keyword(s):  
Factor Xiii ◽  
Coagulation Factor ◽  
Photometric Determination ◽  
Peptide Substrate ◽  
Normal Range ◽  
Step Procedure ◽  
Healthy Donors ◽  
One Step ◽  
Aggregation Inhibitor

SummaryAn assay for a direct photometric determination of F XIII in untreated and undiluted plasma was developed. In a one-step procedure F XIII is activated by thrombin and Ca2+ and crosslinks glycine-ethylester to a specific glutamine containing peptide substrate. The released ammonia is incorporated into α-keto-glutarate by glutamate dehydrogenase, and the NADH consumption of this reaction is measured photometrically at 340 nm. NADH-consumption is directly proportional to the F XIII activity. Fibrin polymerization and the corresponding turbidity is avoided by the use of a fibrin aggregation inhibitor.The procedure is rapid and simple and enables to measure within the range of 0 to 150% F XIII. It can be performed with automated analyzers as well as with common photometric equipment.The normal range of F XIII activity in 167 healthy donors was determined to be 70 to 140%.

Sign in / Sign up

Export Citation Format

Share Document