Colony-Stimulating Activity Production by Hemopoietic Organ Fibroblastoid Cells in vitro

1983 ◽  
Vol 69 (6) ◽  
pp. 369-375 ◽  
Author(s):  
K.G.M. Brockbank ◽  
C.M.J. van Peer
Keyword(s):  
Colony Stimulating Activity ◽  
Hemopoietic Organ

In vitro production of granulocyte–macrophage colony-stimulating activity by murine bone marrow and spleen following vinblastine administrationIN VIVO

1987 ◽  
Vol 5 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Douglas E. Williams ◽  
David S. Chervinsky ◽  
Frank R. Orsini ◽  
Cameron K. Tebbi ◽  
John E. Fitzpatrick
Keyword(s):  
Bone Marrow ◽  
In Vitro Production ◽  
Murine Bone Marrow ◽  
Colony Stimulating Activity ◽  
Macrophage Colony ◽  
Vitro Production

scholarly journals Vascular endothelium as a regulator of granulopoiesis: production of colony-stimulating activity by cultured human endothelial cells

Blood ◽  
1980 ◽  
Vol 56 (6) ◽  
pp. 1060-1067
Author(s):  
PJ Quesenberry ◽  
MA Jr Gimbrone
Keyword(s):  
Endothelial Cells ◽  
Human Blood ◽  
Vascular Endothelium ◽  
Colony Formation ◽  
Primary Cultures ◽  
Blood Monocytes ◽  
Human Endothelial Cells ◽  
Colony Stimulating Activity ◽  
Marrow Cells

Colony-stimulating activity is a regulatory factor(s) that promotes differentiation of hemopoietic stem cells to mature granulocytes and macrophages; in man it has been found that blood monocytes, lymphocytes, and tissue macrophages produce it. In an effort to identify other potenitally physiologic tissue sources of colony- stimulating activity, we have studied the capacity of primary cultures of human vascular endothelial cells to produce colony-stimulating activity. Medium conditioned by incubation with endothelial cultures contained activity that promoted granulocyte-macrophage colony formation of nonadherent human and murine marrow cells. Exposure of endothelial cultures to 0.1–5.0 microgram/ml S. typhosa endotoxin for 6- 72 hr enhanced colony-stimulating activity production. Similarly, incubation of endothelial cells with lysates of human blood granulocytes, or cocultivation with intact granulocytes, resulted in increased colony-stimulating activity levels. In 7–14 day cultures, freshly isolated endothelial cells, incorporated into agar underlayers, consistently stimulated more colony formation by nonadherent human marrow cells than comparable numbers of blood monocytes. These data indicate that: (1) cultured human endothelial cells are a potent source of colony-stimulating activity; (2) they respond to endotoxin and granulocytes and their contents by producing increased amounts of CSA; and (3) they produce morea colony-stimulating activity, than human blood monocytes under standardized conditions in vitro. These observations suggest that the vascular endothelium may play a role in the physiologic regulation of granulopoiesis.

scholarly journals Enhancement of colony-stimulating activity production by lithium

Blood ◽  
1977 ◽  
Vol 49 (2) ◽  
pp. 263-267 ◽  
Author(s):  
WG Harker ◽  
G Rothstein ◽  
D Clarkson ◽  
JW Athens ◽  
JL Macfarlane
Keyword(s):  
Protein Synthesis ◽  
Lung Tissue ◽  
Colony Formation ◽  
Culture System ◽  
Agar Culture ◽  
Colony Stimulating Activity ◽  
New Protein ◽  
Stimulation Of

Since lithium causes granulocytosis in some patients, its effect upon granulocyte production was investigated using mouse marrow in the agar culture system. When lithium was added to semisolid cultures of mouse marrow, there was no stimulation of colony formation in the absence of colony-stimulating activity (CSA). In addition, lithium did not potentiate the action of already formed CSA. However, lithium did stimulate the production of CSA by lung tissue. Lithium enhancement of CSA production was blocked by puromycin, indicating that lithium action required active new protein synthesis. It was concluded that lithium promoted enhanced granulocyte production in vitro by stimulating the synthesis of CSA.

scholarly journals Immunostimulators induce granulocyte/macrophage colony-stimulating activity and block proliferation in a monocyte tumor cell line.

1977 ◽  
Vol 146 (2) ◽  
pp. 611-616 ◽  
Author(s):  
P Ralph ◽  
H E Broxmeyer ◽  
I Nakoinz
Keyword(s):  
Tumor Cell ◽  
Cell Line ◽  
Lipid A ◽  
Tumor Cell Line ◽  
Protein Preparation ◽  
Purified Protein Derivative ◽  
Colony Stimulating Activity ◽  
Latex Beads ◽  
Macrophage Colony

Monocyte tumor cell line PU5-1.8 does not normally produce colony-stimulating activity (CSA) required by granulocyte and macrophage progenitors to proliferate and mature in agar. However, CSA is induced in the culture line by as little as 10 ng/ml endotoxic lipopolysaccharide (LPS), with maximum CSA production and release to the medium between 2 and 3 days of incubation. Derived lipid A, but not alkali-treated LPS, is also active. Induction requires RNA and protein synthesis, but is not blocked by mitomycin C or Colcemid. Other inducers of CSA include Mycobacterium Bacillus Calmette-Guérin, tuberculin protein preparation purified protein derivative, zymosan, and phorbol myristate. All inducing agents are specific inhibitors of the monocyte tumor cell proliferation in vitro. Latex beads, another macrophage-activating agent, are rapidly phagocytosed by PU5-1.8 cells, but neither inhibit growth nor induce CSA.

scholarly journals Identification of lactoferrin as the granulocyte-derived inhibitor of colony-stimulating activity production.

1978 ◽  
Vol 148 (4) ◽  
pp. 1052-1067 ◽  
Author(s):  
H E Broxmeyer ◽  
A Smithyman ◽  
R R Eger ◽  
P A Meyers ◽  
M de Sousa
Keyword(s):  
Binding Protein ◽  
Iron Binding ◽  
Blood Monocytes ◽  
Iron Saturation ◽  
Colony Stimulating Activity ◽  
Monocytes And Macrophages ◽  
Iron Binding Protein ◽  
Macrophage Colony

Lactoferrin (LF), the iron-binding protein present in the specific granules of mature granulocytes has been identified as colony inhibitory factor (CIF) which suppresses granulocyte--macrophage colony stimulating activity (CSA) production by monocytes and macrophages in vitro and rebound granulopoiesis in vivo. Separation of LF and CIF by isoelectric focusing confirmed that the regions of inhibitory activity corresponded in both to a pH of congruent to 6.5. In addition, the purified immunoglobulin fraction of rabbit anti-human LF antiserum, but not rabbit anti-transferrin (TF), inactivated the capacity of LF and CIF to inhibit CSA production, an effect blocked by prior incubation of anti-LF with neutralizing concentrations of LF. Suppression of CSA production correlated with the iron-saturation of LF; APO-LF (depleted of iron) was only active concentrations greater than 10(-7) M, native LF (8% iron saturated) was active at 10(-15) M, and fully iron-saturated LF inhibited at 10(-17) M. Suppression of CSA production occurred within a 1/2-h preincubation period with human blood monocytes but was reversed by bacterial lipopolysaccharide (LPS). This reversal was dependent on the relative concentrations of LF to LPS. Serum TF, a biochemically similar iron-binding protein which is antigenically distinct from LF, was only minimally active at concentrations greater than 10(-6) M. LF did not inhibit exogenously stimulated human granylocyte and macrophage colony-forming cells or erythropoietin-dependent human or murine erythroid colony- or erythroid burst-forming cells. Microgram quantities of LF acted in vivo to inhibit rebound granulopoiesis and CSA production in CD1 and C57Bl/6 mice pretreated with cyclophosphamide. These results strongly implicate LF as a physiological regulator of granulopoiesis.

scholarly journals Relationship of colony-stimulating activity to apparent kill of human colony-forming cells by irradiation and hydroxyurea

Blood ◽  
1976 ◽  
Vol 47 (3) ◽  
pp. 403-411
Author(s):  
HE Broxmeyer ◽  
PR Galbraith ◽  
FL Baker
Keyword(s):  
Cell Cycle ◽  
Bone Marrow Cells ◽  
Agar Medium ◽  
Low Doses ◽  
Proliferative Capacity ◽  
Colony Stimulating Activity ◽  
Relationship Of ◽  
Synthetic Phase ◽  
Effects Of Irradiation On

Suspensions of human bone marrow cells were subjected to 137Cs irradiation in vitro and then cultured in semisolid agar medium. Cultures of irradiated cells were stimulated with colony-stimulating activity (CSA) of different potencies, and it was found that the amount of stimulation applied to cultures influenced the apparent kill of colony-forming cells (CFC). It was also found that the effects of irradiation on colony formation were not confined to CFC kill since medium conditioned by cells during irradiation exhibited stimulatory and inhibitory properties after treatment by 600 and 1000 rads, respectively. Studies in which irradiated cells were pretreated with hydroxyurea indicated that CFC in the DNA synthetic phase of the cell cycle were particularly sensitive to low doses of irradiation. The proliferative capacity of CFC surviving 1000 rads was undiminished as judged by their ability to form large colonies. Estimates of CFC kill by hydroxyurea were also affected by the level of CSA.

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