Effect of Iloprost (ZK 36 374), a Novel Prostacyclin Analogue, on ADP-induced Platelet Aggregation

1985 ◽  
Vol 73 (4) ◽  
pp. 224-227 ◽  
Author(s):  
Hans Wadenvik ◽  
Jack Kutti
Keyword(s):  
Platelet Aggregation ◽  
Prostacyclin Analogue

The Novel Effect of a New Prostacyclin Analogue ZK36374 on the Aggregation of Human Platelets in Whole Blood

1983 ◽  
Vol 50 (03) ◽  
pp. 718-721 ◽  
Author(s):  
A R Saniabadi ◽  
G D O Lowe ◽  
J J F Belch ◽  
C D Forbes ◽  
C R M Prentice ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Whole Blood ◽  
Blood Platelet ◽  
Human Platelets ◽  
The Novel ◽  
Pharmacological Agents ◽  
Prostacyclin Analogue ◽  
Present Technique ◽  
Physiological Approach ◽  
Platelet Aggregates

SummaryPlatelet aggregation was studied at 37° C in citrated whole human blood, using the Ultra Flo 100 Whole Blood Platelet Counter. Aggregation was measured as a fall in the number of single platelets following addition of an aggregating agent. At peak aggregation, the fall in the number of platelets induced by ADP (10 μM), collagen (1 μg/ml) or thrombin (0.2 U/ml) was about 90%. When blood was incubated with the prostacyclin- analogue ZK36374, the aggregation responses to ADP, collagen and thrombin were reduced with IC50’S = 0.5, 1.5 and 3 nM respectively and the corresponding IC100’S were: 1, 3 and 12 nM. When ZK36374 was added at peak aggregation, the number of single platelets increased significantly due to disaggregation of preformed platelet aggregates. It is concluded that the present technique represents a rapid, sensitive and more physiological approach for investigating the effects of pharmacological agents on platelet aggregation.

Effects of Prostacyclin and of the Stable Prostacyclin Analogue ZK 36374 on Forearm Blood Flow and Blood Platelet Behaviour in Man

1985 ◽  
Vol 53 (01) ◽  
pp. 090-094 ◽  
Author(s):  
A J Cowley ◽  
S Heptinstall ◽  
J R Hampton
Keyword(s):  
Blood Flow ◽  
Platelet Aggregation ◽  
Inverse Relationship ◽  
Forearm Blood Flow ◽  
Potent Inhibitor ◽  
Blood Platelet ◽  
Haemodynamic Effects ◽  
Low Doses ◽  
Induce Platelet Aggregation ◽  
Prostacyclin Analogue

SummaryPGI2 and ZK 36374 were each infused into volunteers and the effects on forearm blood flow and on platelet behaviour were determined. Infusions of PGI2 or ZK 36374 did not alter resting forearm blood flow but both agents reduced the extent of the vasoconstriction that occurred in response to cold. ZK 36374 appeared to be a much more potent inhibitor of platelet behaviour than PGI2 when blood was taken while the infusions were in progress, but the effects of both agents were no longer evident one hour after the infusions were terminated. There was an inverse relationship between the extent of cold-induced vasoconstriction and the concentration of sodium arachidonate that was needed to induce platelet aggregation for different individuals. Infusions of PGI2 affected both parameters equally but ZK 36374 had a greater effect on platelet behaviour than on blood flow. It is possible that very low doses of ZK 36374 would result in inhibition of platelet behaviour without producing adverse haemodynamic effects.

Fine Structure of Platelet Interaction with a New Microcrystalline Collagen Preparation

1974 ◽  
Vol 32 ◽  
pp. 58-59
Author(s):  
W. H. Zucker ◽  
R. G. Mason
Keyword(s):  
Fine Structure ◽  
Platelet Aggregation ◽  
Hydrochloric Acid ◽  
Whole Blood ◽  
Platelet Adhesion ◽  
Hemostatic Agent ◽  
Native Collagen ◽  
Fibrillar Morphology ◽  
Clinical Interest ◽  
New Form

Platelet adhesion initiates platelet aggregation and is an important component of the hemostatic process. Since the development of a new form of collagen as a topical hemostatic agent is of both basic and clinical interest, an ultrastructural and hematologic study of the interaction of platelets with the microcrystalline collagen preparation was undertaken.In this study, whole blood anticoagulated with EDTA was used in order to inhibit aggregation and permit study of platelet adhesion to collagen as an isolated event. The microcrystalline collagen was prepared from bovine dermal corium; milling was with sharp blades. The preparation consists of partial hydrochloric acid amine collagen salts and retains much of the fibrillar morphology of native collagen.

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