Detection of Human Lymphocyte Subpopulations by Using Monoclonal Antibodies and Protein A-Coated Erythrocytes

1985 ◽  
Vol 73 (3) ◽  
pp. 140-144 ◽  
Author(s):  
Stela Roman ◽  
I. Moraru ◽  
V. Ghetie
Keyword(s):  
Monoclonal Antibodies ◽  
Human Lymphocyte ◽  
Protein A ◽  
Lymphocyte Subpopulations

Activation of human lymphocyte subpopulations by protein a from staphylococcus aureus bacteria

1979 ◽  
Vol 39 (4) ◽  
pp. 245-255 ◽  
Author(s):  
W. Vogt ◽  
A. Willberg ◽  
G. Bochert ◽  
G. Sieber ◽  
H. R�hl
Keyword(s):  
Staphylococcus Aureus ◽  
Human Lymphocyte ◽  
Protein A ◽  
Lymphocyte Subpopulations

scholarly journals CYTOCHEMISTRY OF HUMAN LYMPHOCYTE SUBPOPULATIONS DELINEATED BY MONOCLONAL ANTIBODIES

1983 ◽  
Vol 53 (2) ◽  
pp. 352-353
Author(s):  
R. J. Armitage ◽  
D. C. Linch ◽  
C. P. Worman ◽  
J. C. Cawley
Keyword(s):  
Monoclonal Antibodies ◽  
Human Lymphocyte ◽  
Lymphocyte Subpopulations

Stimulation of Human Lymphocyte Subpopulations by Protein A from Staphylococcus aureus

1980 ◽  
Vol 61 (4) ◽  
pp. 394-406 ◽  
Author(s):  
Morito Sumiya ◽  
Shogo Kano ◽  
Kazuo Oshimi ◽  
Nobuyuki Gonda ◽  
Fumimaro Takaku
Keyword(s):  
Staphylococcus Aureus ◽  
Human Lymphocyte ◽  
Protein A ◽  
Lymphocyte Subpopulations ◽  
Stimulation Of

Evaluation of the Efficiency of Protein A Affinity Chromatography to Purify a Monoclonal Antibody for Cancer Treatment and its Purity Analysis

2020 ◽  
Vol 7 (2) ◽  
pp. 121-133
Author(s):  
Ayesha Akhtar ◽  
Shivakumar Arumugam ◽  
Shoaib Alam
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Affinity Chromatography ◽  
Protein A ◽  
Exchange Chromatography ◽  
Size Exclusion ◽  
High Yield ◽  
Staphylococcal Protein A ◽  
Purification Step ◽  
Purity Analysis

Background:: Protein A affinity chromatography is often employed as the most crucial purification step for monoclonal antibodies to achieve high yield with purity and throughput requirements. Introduction:: Protein A, also known as Staphylococcal protein A (SPA) is found in the cell wall of the bacteria staphylococcus aureus. It is one of the first discovered immunoglobulin binding molecules and has been extensively studied since the past few decades. The efficiency of Protein A affinity chromatography to purify a recombinant monoclonal antibody in a cell culture sample has been evaluated, which removes 99.0% of feed stream impurities. Materials and Method:: We have systematically evaluated the purification performance by using a battery of analytical methods SDS-PAGE (non-reduced and reduced sample), Cation Exchange Chromatography (CEX), Size-exclusion chromatography (SEC), and Reversed phased-Reduced Chromatography for a CHO-derived monoclonal antibody. Results and Discussion:: The analytical test was conducted to determine the impurity parameter, Host Cell Contaminating Proteins (HCP). It was evaluated to be 0.015ng/ml after the purification step; while initially, it was found to be 24.431ng/ml. Conclusion:: The tests showed a distinct decrease in the level of different impurities after the chromatography step. It can be concluded that Protein A chromatography is an efficient step in the purification of monoclonal antibodies.

scholarly journals Publisher Correction: Washing with alkaline solutions in protein A purification improves physicochemical properties of monoclonal antibodies

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yuichi Imura ◽  
Toshiaki Tagawa ◽  
Yuya Miyamoto ◽  
Satoshi Nonoyama ◽  
Hiroshi Sumichika ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Physicochemical Properties ◽  
Protein A ◽  
Alkaline Solutions

Application of Protein A-Rosette Assay for Screening of Monoclonal Antibodies to Human Complement Regulatory Proteins

1990 ◽  
Vol 7 (2) ◽  
pp. 78-89 ◽  
Author(s):  
Tsukasa Seya ◽  
Tomoko Hara ◽  
Akiko Uenaka ◽  
Eiichi Nakayama ◽  
Hitoshi Akedo
Keyword(s):  
Monoclonal Antibodies ◽  
Protein A ◽  
Regulatory Proteins ◽  
Human Complement ◽  
Complement Regulatory Proteins

Alkaline phosphatase in human lymphocyte subpopulations

1979 ◽  
Vol 35 (2) ◽  
pp. 269-270 ◽  
Author(s):  
R. Foa ◽  
M. T. C. Kramers ◽  
D. Catovsky
Keyword(s):  
Alkaline Phosphatase ◽  
Human Lymphocyte ◽  
Lymphocyte Subpopulations
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