Fibrinolytic Activity of Blood: Photographic Determination of Clot Lysis Time

1959 ◽  
Vol 22 (1) ◽  
pp. 58-64 ◽  
Author(s):  
H. Lackner ◽  
C.C. Goosen
Keyword(s):  
Fibrinolytic Activity ◽  
Clot Lysis ◽  
Lysis Time ◽  
Clot Lysis Time

Influence of Moderate and Strenuous Daily Physical Activity on Fibrinolytic Activity of Blood: Possibility of Plasminogen Activator Stores Depletion

1979 ◽  
Vol 41 (04) ◽  
pp. 745-755 ◽  
Author(s):  
Dušan Keber ◽  
Mojca Stegnar ◽  
Irena Keber ◽  
Bojan Accetto
Keyword(s):  
Physical Activity ◽  
Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
Regular Physical Activity ◽  
Clot Lysis ◽  
Moderate Activity ◽  
Lysis Time ◽  
Strenuous Activity ◽  
Activity Measurements ◽  
Clot Lysis Time

SummaryFibrinolysis was studied in 10 alpinists during regular physical activity of different intensity. Blood was sampled at rest and after exposure to submaximal workload on the treadmill on three occasions: before and after 6 months physical conditioning (moderate physical activity), and after 6 weeks of an alpinistic expedition (strenuous physical activity). Measurements included submaximal working capacity, fibrinogen, euglobulin clot lysis time (ELT), whole plasma clot lysis time, and estimations derived from ELT - percent increase in fibrinolytic activity after exercise (RFS), and absolute increase in fibrinolytic activity after exercise (PAR).Regular moderate activity increased the resting level of ELT, but strenuous activity decreased is. After each treadmill testing, a marked increase in fibrinolytic activity was observed. RFS was unaltered at all three testings. PAR increased after moderate activity, but decreased after strenuous activity.The results indicate that regular physical activity can lead from enhanced to decreased resting activity of plasminogen activator in blood. It is presumed that increased release of activator during prolonged stress causes partial depletion of endothelial stores with the consequence of decreased activator activity in the blood.

Relationship between Plasma Fibrinolytic Activity and Serum Fibrinogen Degradation Products (FDP) Tested by Various Methods

1971 ◽  
Vol 26 (01) ◽  
pp. 083-087 ◽  
Author(s):  
B Lipiński ◽  
A Nowak ◽  
A Odrzywolska ◽  
J Dosiak
Keyword(s):  
Healthy Subjects ◽  
Fibrinolytic Activity ◽  
Degradation Products ◽  
Clot Lysis ◽  
Proteolytic Degradation ◽  
Fibrinogen Degradation Products ◽  
Lysis Time ◽  
Euglobulin Clot Lysis Time ◽  
Fibrinogen Content ◽  
Clot Lysis Time

SummaryIt was found in the present work that the level of serum fibrinogen degradation products (FDP) determined by the immunoassay method correlated well with the staphylococcal clumping titer in serum (correlation coefficient r = 0.68). The content of FDP in serum of 30 healthy subjects and patients with various diseases did not correlate, however, neither with blood fibrinolytic activity estimated by the euglobulin clot lysis time, nor with fibrinogen content and plasma anticlotting activity. It is concluded, that FDP appear in circulation as a result of local proteolytic degradation of intravascularly deposited fibrin without generalized activation of fibrinolysis.

FIBRINOLYTIC ACTION OF RESERPINE IN RATS

1964 ◽  
Vol 42 (1) ◽  
pp. 153-156 ◽  
Author(s):  
J. G. Ashwin ◽  
W. R. Coughlin
Keyword(s):  
Fibrinolytic Activity ◽  
Aminocaproic Acid ◽  
Route Of Administration ◽  
Clot Lysis ◽  
Lysis Time ◽  
Euglobulin Clot Lysis Time ◽  
Epsilon Aminocaproic Acid ◽  
Clot Lysis Time ◽  
Fibrinolytic Action

The plasma fibrinolytic activity in rats was measured by the euglobulin clot lysis time technique after injection of reserpine, serotonin, epsilon-aminocaproic acid, and UML-491. Fibrinolytic activity was increased by reserpine and serotonin, the extent depending on the dosage and route of administration. With serotonin, clot lysis time returned to normal after 6–12 hours. With reserpine, this occurred after 4 days. The antifibrinoiysin E-ACA was able to reduce fibrinolytic activity for more than 24 hours, whether given alone or with reserpine or serotonin. The antiserotonin UML-491 had a paradoxical fibrinolytic enhancing action that lasted for several hours alone, or given with serotonin it increased the fibrinolytic effect of serotonin.

Increased Fibrinolytic Activity during Use of Oral Contraceptives Is Counteracted by an Enhanced Factor XI-independent down Regulation of Fibrinolysis

2000 ◽  
Vol 84 (07) ◽  
pp. 9-14 ◽  
Author(s):  
Saskia Middeldorp ◽  
Winnie Tekelenburg ◽  
Abraham van den Ende ◽  
Guido Tans ◽  
Martin Prins ◽  
...  
Keyword(s):  
Plasminogen Activator ◽  
Oral Contraceptives ◽  
Fibrinolytic Activity ◽  
Tissue Type ◽  
Coagulation System ◽  
Clot Lysis ◽  
Factor Xi ◽  
Lysis Time ◽  
Pai 1 ◽  
Clot Lysis Time

SummaryThe effect of oral contraceptives (OC) on fibrinolytic parameters was investigated in a cycle-controlled cross-over study in which 28 non-OC using women were randomly prescribed either a representative of the so-called second (30 µg ethinylestradiol, 150 µg levonorgestrel) or third generation OC (30 µg ethinylestradiol, 150 µg desogestrel) and who switched OC after a two month wash out period. During the use of OC, the levels of tissue-type plasminogen activator (tPA) activity, plasminogen, plasmin-α2-antiplasmin complexes and D-dimer significantly increased (by 30 to 80%), while the levels of plasminogen activator inhibitor-1 (PAI-1) antigen, PAI-1 activity and tPA antigen significantly decreased (25 to 50%), suggesting an increase in endogenous fibrinolytic activity. These OC-induced changes were not different between the two contraceptive pills. TAFI (thrombin-activatable fibrinolysis inhibitor) levels increased on levonorgestrel, and even further increased on desogestrel. A clot lysis assay that probes both fibrinolytic activity and the efficacy of the coagulation system to generate thrombin necessary to down regulate fibrinolysis via TAFI showed no change of the clot lysis time during OC use. This finding suggests that the OC-induced increase in endogenous fibrinolytic activity is counteracted by an increased capacity of the coagulation system to down regulate fibrinolysis via TAFI. Indeed we observed that during OC use there was a significant increase of F1+2 generation during clot formation. When these assays were performed in the presence of an antibody against factor XI, we observed that the clot lysis time was significantly increased during OC use and that the increase in F1+2 generation during OC therapy was due to a factor XI-independent process, which was significantly higher on desogestrel than on levonorgestrel. These data indicate that the OC-induced inhibition of endogenous fibrinolysis takes place in a factor XI-independent way and is more pronounced on desogestrel than on levonorgestrel-containing OC.

The Effect of Plasma Fibrinogen Levels on Measures of Fibrinolytic Activity

1978 ◽  
Vol 39 (02) ◽  
pp. 450-454 ◽  
Author(s):  
R Chakrabarti ◽  
W R S North ◽  
T W Meade
Keyword(s):  
Multiple Regression ◽  
Fibrinolytic Activity ◽  
Blood Clot ◽  
Plasma Fibrinogen ◽  
Clot Lysis ◽  
Lysis Time ◽  
Fibrin Plate ◽  
Plate Area ◽  
Dilute Blood ◽  
Clot Lysis Time

SummaryIt has been claimed that lysis time methods may be inappropriate as measures of fibrinolytic activity on the grounds that they are largely determined by plasma fibrinogen levels. The dilute blood clot lysis time (DBCLT) has therefore been measured in 103 subjects; fibrinolytic activity (expressed as 100/DBCLT) has been compared with the fibrin plate area (FPA) lysed by the euglobulin fraction, the latter method being, of course, virtually independent of plasma fibrinogen. Plasma fibrinogen levels have also been measured; they ranged from 167 to 416 mg%. Results have been analysed by the technique of multiple regression. The incorporation of plasma fibrinogen levels in the regression equation relating 100/DBCLT to FPA does not result in a significant increase in the proportion of the variance in 100/DBCLT that can be explained. It is concluded that there is no evidence that DBCLT is influenced by plasma fibrinogen levels within the physiological range.

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