Intrinsic Factor and Gastric Juice Secretion Induced by Pentagastrin

Digestion ◽  
1967 ◽  
Vol 108 (5) ◽  
pp. 268-274 ◽  
Author(s):  
S. Tura ◽  
P.R. Dal Monte ◽  
F. Possati ◽  
E. Fiore
Keyword(s):  
Gastric Juice ◽  
Intrinsic Factor ◽  
Gastric Juice Secretion

scholarly journals Rapid Charcoal Assay for Intrinsic Factor (IF), Gastric Juice Unsaturated B12 Binding Capacity, Antibody to IF, and Serum Unsaturated B12 Binding Capacity

Blood ◽  
1965 ◽  
Vol 25 (6) ◽  
pp. 875-884 ◽  
Author(s):  
CHESTER GOTTLIEB ◽  
KAM-SENG LAU ◽  
LOUIS R. WASSERMAN ◽  
VICTOR HERBERT
Keyword(s):  
Small Molecules ◽  
Gastric Juice ◽  
Rapid Method ◽  
Molecular Sieve ◽  
Clinical Laboratory ◽  
Binding Capacity ◽  
Intrinsic Factor ◽  
Wide Range ◽  
Charcoal Particle ◽  
Ready Availability

Abstract The principle of the dialysis assays for intrinsic factor (IF) and antibody to IF was integrated with the demonstration that protein-coated charcoal adsorbs only free and not bound B12, in a single rapid method for 4 separate assays: (1) assay of IF, (2) assay of gastric juice unsaturated B12 binding capacity, (3) assay of serum antibodies to IF and (4) assay of serum unsaturated B12 binding capacity. The method is sensitive, accurate, reproducible, and can easily be completed within an hour. The simplicity and ready availability of reagents and equipment lends itself to ready adoption in any clinical laboratory using radioisotopes. The charcoal particle is considered as a solid microsponge, and its coat of albumin, other protein, carbohydrate, or other large molecule as a molecular sieve surrounding the sponge. The whole constitutes a system of "instant dialysis," with a wide range of applications in the separation of large from small molecules.

scholarly journals On the Nature of Castle’s Hemopoietic Factor

Blood ◽  
1951 ◽  
Vol 6 (12) ◽  
pp. 1234-1239 ◽  
Author(s):  
SHEILA T. CALLENDER ◽  
L. G. LAJTHA
Keyword(s):  
Vitamin B12 ◽  
Gastric Juice ◽  
Intrinsic Factor ◽  
Normal Serum ◽  
Relationship Of ◽  
The Relationship

Abstract 1. Normal gastric juice (intrinsic factor) and vitamin B12 together form a thermolabile hemopoietic factor which ripens megaloblasts in vitro, both gastric juice and B12 alone being inactive. 2. The hemopoietic factor in normal serum which ripens megaloblasts in vitro also appears to be thermolabile, heating to 56 C. for 2 hours destroying some of its activity. 3. The relationship of these factors is discussed and an extra-gastric as well as a gastric source of intrinsic factor is postulated.

Functional activity of intrinsic factor measured by using solubilized receptor protein.

1982 ◽  
Vol 28 (8) ◽  
pp. 1794-1796 ◽  
Author(s):  
G Marcoullis ◽  
S P Rothenberg
Keyword(s):  
Gastric Juice ◽  
No Value ◽  
Functional Activity ◽  
Sodium Sulfate ◽  
Intrinsic Factor ◽  
Receptor Site ◽  
Final Concentration ◽  
Receptor Protein ◽  
Species Specific ◽  
Protein Reagent

Abstract The traditional radioimmunoassay for gastric intrinsic factor, in which this protein is measured on the basis of immunoreactivity rather than function, is of no value for identifying intrinsic factor that binds cobalamin but does not bind to the ileal receptor site, or for detecting animal intrinsic factor, which does not cross react with human intrinsic factor. Accordingly, we have applied a radioassay for the intrinsic factor receptor protein to measure the functional activity of intrinsic factor in gastric juice. The receptor protein reagent was partly purified from guniea pig ilea and its interaction with intrinsic factor--CN[57Co]-cobalamin was determined by precipitation with sodium sulfate at a final concentration of 150 g/L. Results of this assay were comparable with results obtained for intrinsic factor by radioimmunoassay. The receptor protein did not bind immunoreactive intrinsic factor that was functionally abnormal. This functional radioassay for intrinsic factor is not species specific and will be of value when specific antiserum to intrinsic factor is not available and when cobalamin malabsorption is to be evaluated in patients who are secreting normal amounts of immunoreactive intrinsic factor.

Intrinsic factor secretion in the gastric juice in diabetes mellitus

1973 ◽  
Vol 18 (2) ◽  
pp. 85-91 ◽  
Author(s):  
T. Kanaghinis ◽  
N. Iatromanolakis ◽  
D. Ikkos ◽  
P. Gatsou ◽  
C. Gardikas
Keyword(s):  
Diabetes Mellitus ◽  
Gastric Juice ◽  
Intrinsic Factor ◽  
Factor Secretion

Study on Gastric Juice Secretion by Electroacupuncture at Zusanli Point on Rabbits under Action of Cimetidine

2011 ◽  
Vol 393-395 ◽  
pp. 972-975
Author(s):  
Yong Peng ◽  
Jian Li
Keyword(s):  
Gastric Juice ◽  
Acupuncture Group ◽  
H2 Receptor ◽  
Before And After ◽  
Gastric Juice Secretion

The effect and mechanism on gastric juice secretion by electroacupuncturing at Zusanli points on rabbits with modern apparatuses were studied. Zusanli points were stimulated with electroacupunctures to observe the flux of gastric juice in the study. Before and after Cimetidine being injected by the pathway of points, Zusanli points were respectively stimulated with electroacupunctures to observe the flux of gastric juice secretion. The results showed that the flux of gastric juice slowered (P0.05) when the stopping acupuncture group for 10 minutes compared with the during acupuncturing group, but after Cimetidine being injected, the flux of gastric juice secretion did not increase obviously (P>0.05) during acupuncturing for 10 minutes compared with the no acupuncturing group, and also did not increase (P>0.05) stopping acupuncture for 10 minutes compared with no acupuncture. So the study showed that the flux of gastric juice secretion could be increased by electroacupuncturing at Zusanli points, and the mechanism may be come true by the gastric H2 receptor pathway.

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