Insulin Stimulates Production of Glycoconjugate Layers on the Cell Surface of Gastric Surface Mucous Cell Line GSM06

Digestion ◽  
1997 ◽  
Vol 58 (1) ◽  
pp. 28-33 ◽  
Author(s):  
Yoshiaki Tabuchi ◽  
Norifumi Sugiyama ◽  
Tadashi Horiuchi ◽  
Kazuhisa Furuhama ◽  
Mitsuru Furusawa
Keyword(s):  
Cell Surface ◽  
Cell Line ◽  
Mucous Cell ◽  
Surface Mucous Cell

scholarly journals Insulin stimulates production of glyco-conjugate layers on the cell surface of gastric surface mucous cell line GSM06.

1996 ◽  
Vol 71 ◽  
pp. 108
Author(s):  
Yoshiaki Tabuchi ◽  
Norifumi Sugiyama ◽  
Tadashi Horiuchi ◽  
Kazuhisa Furuhama
Keyword(s):  
Cell Surface ◽  
Cell Line ◽  
Mucous Cell ◽  
Surface Mucous Cell

Yeast KEX2-like protease furin disappears with the maturation of the gastric surface mucous cell line GSM06

1995 ◽  
Vol 108 (4) ◽  
pp. A734
Author(s):  
Y. Konda ◽  
T. Kayo ◽  
Y. Sawada ◽  
T. Horiuchi ◽  
N. Sugiyama ◽  
...  
Keyword(s):  
Cell Line ◽  
Mucous Cell ◽  
Surface Mucous Cell

scholarly journals Biosynthesis and assembly of platelet GPIIb-IIIa in human megakaryocytes: evidence that assembly between pro-GPIIb and GPIIIa is a prerequisite for expression of the complex on the cell surface

Blood ◽  
1989 ◽  
Vol 74 (5) ◽  
pp. 1603-1611 ◽  
Author(s):  
A Duperray ◽  
A Troesch ◽  
R Berthier ◽  
E Chagnon ◽  
P Frachet ◽  
...  
Keyword(s):  
Cell Surface ◽  
Cell Line ◽  
Platelet Membrane ◽  
Membrane Glycoproteins ◽  
Liquid Cultures ◽  
Calcium Dependent ◽  
Large Pool ◽  
Adhesive Proteins ◽  
Kinetics Of ◽  
Megakaryocytic Cell

Abstract The platelet membrane glycoproteins GPIIb and GPIIIa form a calcium- dependent heterodimer that functions as a receptor for adhesive proteins on stimulated platelets. In this study, we have investigated the kinetics of the assembly reaction that result in GPIIb-IIIa dimerization. Pulse-chase experiments analysis performed on human megakaryocytes obtained from liquid cultures of chronic myelogenous leukemic patients with antibodies specific for GPIIIa or GPIIb demonstrated the existence of a pro-GPIIb-GPIIIa complex and of a large pool (60%) of unassociated GPIIIa; nearly all the GPIIb and the pro- GPIIb molecules were found associated with GPIIIa. This free GPIIIa was not exposed on the cell surface. Pulse-chase experiments on a subclone of the human megakaryocytic cell line LAMA-84 revealed that the cells from this subclone produced only the pro-GPIIb, which was neither processed into mature GPIIb nor expressed on the cell surface. The expression of GPIIIa in PMA treated cells resulted in the production of the mature GPIIb form and the expression of the GPIIb-IIIa complex on the cell surface. These results indicate that assembly between the early forms of pro-GPIIb and GPIIIa is an obligatory step for the maturation of the heterodimer and its expression on the cell surface.

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