Direct Inhibitory Effect of Neurotensin on Isolated Gastric Smooth Muscle Cells of Guinea Pig via the Cyclic GMP System

Digestion ◽  
1993 ◽  
Vol 54 (3) ◽  
pp. 135-138
Author(s):  
Yoshiharu Chijiiwa ◽  
Osamu Kawakami ◽  
Tadashi Misawa ◽  
Teppei Kabemura ◽  
Hajime Nawata
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Cyclic Gmp ◽  
Muscle Cells ◽  
Inhibitory Effect ◽  
Gastric Smooth Muscle ◽  
Direct Inhibitory Effect

Direct Inhibitory Effect of Thyrotropin-Releasing Hormone on Isolated Cecal Circular Smooth Muscle Cells of Guinea Pig

Digestion ◽  
1996 ◽  
Vol 57 (2) ◽  
pp. 79-82 ◽  
Author(s):  
Naohiko Harada ◽  
Yoshiharu Chijiiwa ◽  
Hirotada Akiho ◽  
Hiroaki Okabe ◽  
Hajime Nawata
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Inhibitory Effect ◽  
Thyrotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Circular Smooth Muscle ◽  
Direct Inhibitory Effect

DIRECT INHIBITORY EFFECT OF ADRENOMEDULLIN AND GUANYLIN ON ISOLATED CAECAL CIRCULAR SMOOTH MUSCLE CELLS OF GUINEA PIG

Life Sciences ◽  
1997 ◽  
Vol 61 (15) ◽  
pp. 1479-1485 ◽  
Author(s):  
Toshiaki Ochiai ◽  
Yoshiharu Chijiiwa ◽  
Yasuaki Motomura ◽  
Yuji Iwakiri ◽  
Hajime Nawata
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Inhibitory Effect ◽  
Circular Smooth Muscle ◽  
Direct Inhibitory Effect

Smooth muscle cells from guinea pig stomach possess high-affinity galanin receptors that mediate relaxation

1994 ◽  
Vol 266 (5) ◽  
pp. G839-G845 ◽  
Author(s):  
Z. F. Gu ◽  
T. K. Pradhan ◽  
D. H. Coy ◽  
R. T. Jensen
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Maximal Effect ◽  
Dependent Manner ◽  
High Affinity ◽  
Gastric Smooth Muscle ◽  
Guinea Pig Stomach ◽  
Galanin Receptors

Galanin-like immunoactivity occurs in nerves and plexi in muscle layers throughout gastrointestinal tract including the stomach. Galanin can affect gastric emptying and contraction or relaxation of gastric muscle in different species. The aim of this study was to investigate the direct effect of galanin on dispersed gastric smooth muscle cells and to characterize any galanin receptors that mediated any effect. Dispersed gastric smooth muscle cells were prepared from guinea pig stomach by collagenase digestion. Porcine galanin (p-galanin; 1 microM) did not stimulate contraction when present alone; however, p-galanin (1 microM) inhibited carbachol-induced contraction with a half-maximal effect at 7 nM. p-Galanin (1 microM) increased cellular adenosine 3',5'-cyclic monophosphate (cAMP) content by 10 s and caused a maximal increase of 80% over basal. 125I-galanin (porcine) bound to dispersed cells in a time- and temperature-dependent manner. Binding was saturable, reversible, and specific. Binding of 125I-galanin was inhibited almost equally by porcine and rat galanin (Ki = 6-8 nM) but was not inhibited by the galanin-associated peptide [preprogalanin-(108-123)]. The fragment galanin-(1-16) was equally potent to rat galanin; however, the fragment galanin-(9-29) was 56-fold less potent (Ki = 370 nM). Computer analysis demonstrated there were two binding sites for p-galanin on gastric smooth muscle cells, a high-affinity site (Kd = 2.6 nM) with low capacity (Bmax = 175 fmol/mg protein) and a low-affinity site (Kd = 150 nM) with large capacity (Bmax = 3,611 fmol/mg protein).(ABSTRACT TRUNCATED AT 250 WORDS)

Receptors on smooth muscle cells: characterization by contraction and specific antagonists

1982 ◽  
Vol 242 (4) ◽  
pp. G400-G407 ◽  
Author(s):  
K. N. Bitar ◽  
G. M. Makhlouf
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Low Doses ◽  
Response Curves ◽  
High Affinity ◽  
Gastric Smooth Muscle ◽  
Dose Dependent ◽  
Cck Receptor Antagonists

Smooth muscle cells were isolated from the stomach of the guinea pig, and the kinetics, stoichiometry, and specificity of contraction in response to the C-terminal octapeptides of cholecystokinin (CCK-OP), gastrin-17, and acetylcholine were examined. All three agonists elicited dose-dependent peak contraction that did not depend on the presence of extra-cellular calcium. The potencies of CCK-OP and gastrin-17 were equal (D50, 10(-11) M) and 10 times greater than the potency of acetylcholine (D50, 10(-10) M). A combination of low doses of acetylcholine and CCK-OP was synergistic; however, its effect did not exceed the maximal responses to either agonists alone or to high extracellular concentrations of calcium. The specificity of the receptors was established by the use of atropine and the two CCK-receptor antagonists dibutyryl cGMP and proglumide. The span of the dose-response curves was wide, suggesting the existence of receptor heterogeneity. It is concluded that gastric smooth muscle cells of the guinea pig possess distinct, high-affinity receptors for CCK-gastrin and acetylcholine; the receptors mediate contraction that is not immediately dependent on the presence of extracellular calcium.

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