Mechanisms of Antisecretory Action of Intragastric FPL-52694: A Mast Cell Stabilizer in Anesthetized Rats

Digestion ◽  
1986 ◽  
Vol 34 (4) ◽  
pp. 259-267 ◽  
Author(s):  
Koji Takeuchi ◽  
Shigeru Ueki ◽  
Susumu Okabe
Keyword(s):  
Mast Cell ◽  
Mast Cell Stabilizer ◽  
Anesthetized Rats

Mucosal mast cells are involved in CCK disruption of MMC in the rat intestine

1998 ◽  
Vol 275 (1) ◽  
pp. G63-G67 ◽  
Author(s):  
Carme Juanola ◽  
Magda Giralt ◽  
Marcel Jiménez ◽  
Marisabel Mourelle ◽  
Patri Vergara
Keyword(s):  
Mast Cell ◽  
Small Intestine ◽  
Mast Cells ◽  
Control Group ◽  
Rat Intestine ◽  
Pancreatic Acini ◽  
Mast Cell Stabilizer ◽  
Mucosal Mast Cells ◽  
Stimulation Of ◽  
Cck Release

Our aim was to determine if mucosal mast cells could be activated by endogenous CCK and, as a consequence, mediate CCK actions in the small intestine. Rats were prepared for electromyography to record electrical activity in the small intestine. In another group of animals, the duodenum was perfused to measure rat mast cell protease II (RMCP II) as indicative of mast cell degranulation. Endogenous CCK release was induced by administration of soybean trypsin inhibitor (SBTI) in conscious rats or by intraduodenal perfusion of ovalbumin hydrolysate (OVH) in anesthetized rats. CCK concentration was measured by bioassay on pancreatic acini. SBTI in control rats disrupted migrating motor complexes (MMC) for >40 min. In rats treated with the mast cell stabilizer ketotifen, SBTI did not induce any change in the MMC pattern. RMCP II concentration in the duodenal perfusate significantly increased after OVH. Perfusate from ketotifen-treated animals did not show any significant increase in RMCP II values during OVH perfusion, although CCK plasma concentration was not different from the control group. Furthermore, infusion of the CCK-B receptor antagonist L-365,260 significantly blocked the increase of RMCP II concentration after OVH. Our results indicate that mucosal mast cells are degranulated by endogenous CCK release through stimulation of CCK-B receptors. Therefore mucosal mast cells participate in CCK intestinal actions.

scholarly journals The Mast Cell Stabilizer Ketotifen Fumarate Lessens Contracture Severity and Myofibroblast Hyperplasia

2010 ◽  
Vol 92 (6) ◽  
pp. 1468-1477 ◽  
Author(s):  
Michael J Monument ◽  
David A Hart ◽  
A Dean Befus ◽  
Paul T Salo ◽  
Mei Zhang ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cell Stabilizer ◽  
Ketotifen Fumarate

scholarly journals Topical Application of a Mast Cell Stabilizer Improves Impaired Diabetic Wound Healing

2020 ◽  
Vol 140 (4) ◽  
pp. 901-911.e11 ◽  
Author(s):  
Ana Tellechea ◽  
Sha Bai ◽  
Seema Dangwal ◽  
Georgios Theocharidis ◽  
Masa Nagai ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mast Cell ◽  
Topical Application ◽  
Diabetic Wound ◽  
Mast Cell Stabilizer ◽  
Diabetic Wound Healing

scholarly journals Design of Potential Pharmaceutical-Based Metal Complexes Derived from Cromolyn a Mast Cell Stabilizer

ACS Omega ◽  
2020 ◽  
Vol 5 (46) ◽  
pp. 29714-29721
Author(s):  
Israel Rodríguez ◽  
Jeaninna Flores Bello ◽  
Joyce Marie Serrano Valcárcel ◽  
Vilmalí López-Mejías
Keyword(s):  
Mast Cell ◽  
Metal Complexes ◽  
Mast Cell Stabilizer

scholarly journals Mast cells degranulation affects angiogenesis in the rat uterine cervix during pregnancy

Reproduction ◽  
2004 ◽  
Vol 127 (3) ◽  
pp. 379-387 ◽  
Author(s):  
J Varayoud ◽  
J G Ramos ◽  
V L Bosquiazzo ◽  
M Muñoz-de-Toro ◽  
E H Luque
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Blood Vessels ◽  
Uterine Cervix ◽  
Vascular System ◽  
Mast Cell Degranulation ◽  
Endothelial Cell Proliferation ◽  
Disodium Cromoglycate ◽  
Mast Cell Stabilizer ◽  
Vascular Area

During pregnancy, it is essential that sufficient nutrients are supplied by the vascular system to support the dramatic modifications of the rat uterine cervix. Angiogenesis refers to the growth of new blood vessels from pre-existing microcirculation and mast cells have been associated with this process. This study examined the modifications of the vascular compartment and the distribution of mast cells on cervical tissue during pregnancy. Using disodium cromoglycate as a mast cell stabilizer, we determined the effects of the mast cell degranulation on cervical angiogenesis. Mast cell distribution and their degranulation status were evaluated by immunohistochemistry. Endothelial cell proliferation was measured by bromodeoxyuridine incorporation. Vascular areas (absolute and relative) and maturation indices were assessed by quantitative immunohistochemistry of von Willebrand factor and α-smooth muscle actin respectively. Mast cells were predominantly observed during the first half of pregnancy in the perivascular zones. The values of bromodeoxyuridine incorporation, absolute vascular area and vascular maturation index exhibited a significant increase throughout pregnancy. All animals that received mast cell stabilizer showed more than 40% of non-degranulated mast cells. Treated rats exhibited a decrease in endothelial proliferation and in relative vascular area; in addition, a large proportion of mature blood vessels was observed, suggesting a diminished level of new vessel formation. The effects of the mast cell stabilizer were sustained beyond the end of treatment. This is the first report that brings evidence that mast cell degranulation could be a necessary process to contribute to the normal angiogenesis of the rat cervix during pregnancy. Further investigations are needed to elucidate the possible implications of abnormal vascular development of the uterine cervix on the physiological process of ripening and parturition.

scholarly journals TLR7 promotes smoke-induced lung damage through the activity of mast cell tryptase

2020 ◽  
Author(s):  
Tatt Jhong Haw ◽  
Malcolm Starkey ◽  
Stelios Pavlidis ◽  
Sheena Tam ◽  
Prema M. Nair ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cell Activity ◽  
Lung Damage ◽  
Chronic Obstructive ◽  
Toll Like Receptor ◽  
Mast Cell Tryptase ◽  
Obstructive Pulmonary Disease ◽  
Mast Cell Stabilizer ◽  
Tlr7 Agonist ◽  
Deficient Mice

Abstract Toll-like receptor (TLR)7 is known for eliciting immunity against single-stranded RNA viruses. TLR7 was increased in both human and cigarette smoke (CS)-induced experimental chronic obstructive pulmonary disease (COPD). Severity of CS-induced emphysema and COPD was reduced in TLR7-deficient mice whilst inhalation of imiquimod (TLR7-agonist) induced emphysema in naïve mice. Imiquimod-induced emphysema was reduced in mice treated with mast cell stabilizer cromolyn or deficient in mast cell protease-6. Therapeutic treatment with anti-TLR7 monoclonal antibody suppressed CS-induced emphysema, experimental COPD and accumulation of pulmonary mast cells. We demonstrate an unexpected role for TLR7 in mediating emphysema and COPD through mast cell activity.

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