scholarly journals Determination of Radical Species in the Kidney of Rats with Chronic Renal Failure by the Spin Trapping Method

Nephron ◽  
1995 ◽  
Vol 70 (3) ◽  
pp. 382-384 ◽  
Author(s):  
Takako Yokozawa ◽  
Naoki Fujitsuka ◽  
Hikokichi Oura ◽  
Akitane Mori ◽  
Hiroshi Kashiwagi
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Spin Trapping ◽  
Radical Species ◽  
Trapping Method

scholarly journals Measurement of strontium in serum, urine, bone, and soft tissues by Zeeman atomic absorption spectrometry

1997 ◽  
Vol 43 (1) ◽  
pp. 121-128 ◽  
Author(s):  
Patrick C D’Haese ◽  
Glen F Van Landeghem ◽  
Ludwig V Lamberts ◽  
Vera A Bekaert ◽  
Iris Schrooten ◽  
...  
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Soft Tissues ◽  
Absorption Spectrometry ◽  
Serum Samples ◽  
Tissue Samples ◽  
Triton X

Abstract To study the possible accumulation of Sr in chronic renal failure patients, methods were developed for the determination of the element in serum, urine, bone, and soft tissues by using Zeeman atomic absorption spectrometry. Serum samples were diluted 1:4 with a Triton X-100–HNO3 mixture, whereas urine samples were diluted 1:20 with HNO3. Bone samples were digested with concentrated HNO3 in stoppered polytetrafluoroethylene (Teflon®) tubes, whereas soft tissues were dissolved in a tetramethylammonium hydroxide solution in water. For serum and urine we used matrix-matched calibration curves, whereas bone and tissue samples were measured against aqueous calibrators. Atomization was performed from the wall of pyrolytically coated graphite tubes for all of the matrices under study. Both inter- and intraassay CVs were <6% (n = 12, n = 10, respectively), and the recovery of added analyte was close to 100% for all of the biological matrices under study. Detection limits were 1.2 μg/L (serum), 0.3 μg/L (urine), 0.4 μg/g (bone), and 2.2 ng/g (soft tissues), whereas the sensitivity determined by the slope of the calibration curve, i.e., the amount of Sr producing a 0.0044 integrated absorbance change in signal, was 2.4 pg, 2.4 pg, 3.9 pg, and 2.6 pg for these matrices respectively. We conclude that the present methods are precise and accurate and easily applicable for both routine use and research investigations. They will allow us to study the metabolism of the element in chronic renal failure patients and shed some light on the association that was recently noted between increased bone Sr concentrations and the development of osteomalacia in these individuals.

Allantoin as a marker of oxidative stress in human erythrocytes

2008 ◽  
Vol 46 (9) ◽  
Author(s):  
Roman Kand'ár ◽  
Pavla Žáková
Keyword(s):  
Oxidative Stress ◽  
Renal Failure ◽  
Uric Acid ◽  
Chronic Renal Failure ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Control Group ◽  
Uric Acid Concentration ◽  
Reversed Phase Hplc

Abstract: Uric acid is the final product of purine metabolism in humans. It was determined that this compound has important antioxidative properties and it may be oxidized to allantoin by various reactive oxygen species. Therefore, the measurement of allantoin may be useful for the determination of oxidative stress in humans.: We measured allantoin and uric acid in human plasma and erythrocytes obtained from patients with chronic renal failure before hemodialysis (n=30) and blood donors (n=30). We used a method based on selective isolation of allantoin from deproteinized plasma and erythrocyte lysate samples on AG 1-X8 resin and its derivatization to glyoxylate-2, 4-dinitrophenylhydrazone. Separation of glyoxylate-2, 4-dinitrophenylhydrazone from interfering substances was achieved on reversed phase HPLC with gradient elution and UV/VIS detection at 360 nm. Uric acid was determined by reversed phase HPLC with UV/VIS detection at 292 nm.: We found significant differences in allantoin and uric acid concentration between the patients with chronic renal failure and the control group both in plasma (20.5±6.5 μmol/L and 323.9±62.9 μmol/L vs. 2.1±1.1 μmol/L and 270.1±62.3 μmol/L, p<0.05) and erythrocytes [82.8±39.1 nmol/g hemoglobin (Hb) and 110.7±28.8 nmol/g Hb vs. 20.1±6.1 nmol/g Hb and 82.1±23.7 nmol/g Hb, p<0.05].: Significant higher levels of allantoin in both plasma and erythrocytes of patients with chronic renal failure indicate that allantoin may be used as a good marker of oxidative stress.Clin Chem Lab Med 2008;46:1270–4.

Ion chromatographic determination of plasma oxalate in healthy subjects, in patients with chronic renal failure and in cases of hyperoxaluric syndromes

1990 ◽  
Vol 511 ◽  
pp. 223-231 ◽  
Author(s):  
Michele Petrarulo ◽  
Ornella Bianco ◽  
Martino Marangella ◽  
Sergio Pellegrino ◽  
Franco Linari ◽  
...  
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Healthy Subjects ◽  
Chromatographic Determination

Quantitative Determination of Immunoglobulins in Chronic Renal Failure

1975 ◽  
Vol 53 (6) ◽  
pp. 336-340 ◽  
Author(s):  
N. Kaliakmanis ◽  
K. Melissinos ◽  
I. Economidou ◽  
K. Vlassopoulos
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Quantitative Determination

scholarly journals Identification and determination of the prevalence of Toxoplasma gondii in patients with chronic renal failure by ELISA and PCR

2016 ◽  
Vol 6 (5) ◽  
pp. 347-349 ◽  
Author(s):  
Babak Rezavand ◽  
Abbas Mahmoodzadeh Poornaki ◽  
Khojasteh Rahim Mokhtari ◽  
Alireza Mohammad ◽  
Ammar Andalibian ◽  
...  
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Toxoplasma Gondii
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