Effect of Peritoneal Dialysis Effluent on Superoxide Anion Production by Polymorphonuclear Neutrophils

Nephron ◽  
1993 ◽  
Vol 64 (3) ◽  
pp. 382-387 ◽  
Author(s):  
I. Daniels ◽  
M. Lindsay ◽  
C. Porter ◽  
A.P. Haynes ◽  
J. Fletcher ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Superoxide Anion ◽  
Polymorphonuclear Neutrophils ◽  
Superoxide Anion Production ◽  
Anion Production ◽  
Peritoneal Dialysis Effluent

scholarly journals Opsonization of Actinobacillus actinomycetemcomitans by Immunoglobulin G Antibody Reactive with Phosphorylcholine

2002 ◽  
Vol 70 (11) ◽  
pp. 6485-6488 ◽  
Author(s):  
Donald Purkall ◽  
John G. Tew ◽  
Harvey A. Schenkein
Keyword(s):  
Streptococcus Pneumoniae ◽  
Immunoglobulin G ◽  
Oxidative Burst ◽  
Superoxide Anion ◽  
Polymorphonuclear Neutrophils ◽  
Actinobacillus Actinomycetemcomitans ◽  
Superoxide Anion Production ◽  
Protective Antibody ◽  
Anion Production ◽  
Human Sera

ABSTRACT We used two strains of Actinobacillus actinomycetemcomitans, one bearing phosphorylcholine (PC) (strain D045D-40) and one devoid of PC antigens (strain DB03A-42), as well as a nonencapsulated strain of Streptococcus pneumoniae (strain 39937), to examine the opsonic properties of physiological concentrations (⩽30 μg/ml) of purified human anti-PC immunoglobulin G (IgG). Anti-PC bound to both A. actinomycetemcomitans DO45D-40 and S. pneumoniae 39937 and induced superoxide anion production by polymorphonuclear neutrophils; induction of the oxidative burst was inhibited by antibodies to either CD16 or CD32. Thus, anti-PC IgG at concentrations present in most human sera promotes the opsonization of PC-expressing strains of A. actinomycetemcomitans in the absence of complement, implying that anti-PC may be a protective antibody against such strains of bacteria.

Plasma-Mediated Neutrophil Activation during Acute Myocardial Infarction: Role of Platelet-Activating Factor

1995 ◽  
Vol 89 (2) ◽  
pp. 171-176 ◽  
Author(s):  
Tomasz Siminiak ◽  
Robin M. Egdell ◽  
Daniel J. O'Gorman ◽  
Julian F. Dye ◽  
Desmond J. Sheridan
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Superoxide Anion ◽  
Platelet Activating Factor ◽  
Neutrophil Activation ◽  
Polymorphonuclear Neutrophils ◽  
Polymorphonuclear Neutrophil ◽  
Plasma Samples ◽  
Superoxide Anion Production ◽  
Anion Production

1. Polymorphonuclear neutrophils are involved in the development of myocardial injury during ischaemia through the release of free oxygen radicals and by adhesion of activated polymorphonuclear neutrophils to endothelium, resulting in plugging of coronary capillaries. Polymorphonuclear neutrophil activation may be a result of contact with ligands expressed by endothelial cells and/or a response to soluble stimuli released from ischaemic tissue to the plasma. 2. To investigate this we studied plasma-mediated polymorphonuclear neutrophil activation in vitro using plasma samples collected from 14 patients with acute myocardial infarction at time of admission and 6 h and 1, 2, 5 and 7 days later. Plasma samples were incubated with washed polymorphonuclear neutrophils isolated from healthy donors. Expression of adhesion molecules CD18/CD11b integrin and L-selectin (Leu-8) were measured by flow cytometry and superoxide anion production in polymorphonuclear neutrophils was measured by chemiluminescence. 3. Plasma samples obtained 6 h and 1 day after admission were capable of inducing CD18/CD11b antigen expression, superoxide anion production and L-selectin shedding in the washed polymorphonuclear neutrophils, and this effect was significant when compared with plasma taken at 5 and 7 days after admission. 4. The plasma-mediated polymorphonuclear neutrophil stimulation was prevented when the PMN were pretreated with platelet-activating factor receptor antagonists BN52021 or BN50739. The platelet-activating factor concentrations detected in the plasma samples were not higher than those detected in plasma from healthy subjects. 5. These findings suggest that during acute myocardial infarction peripheral plasma contains soluble stimuli capable of inducing polymorphonuclear neutrophil integrin expression, L-selectin shedding and oxygen free radical production and that platelet-activating factor appears to act as an autocrine polymorphonuclear neutrophil stimulus.

scholarly journals Collagen activates superoxide anion production by human polymorphonuclear neutrophils

1987 ◽  
Vol 246 (3) ◽  
pp. 599-603 ◽  
Author(s):  
J C Monboisse ◽  
G Bellon ◽  
J Dufer ◽  
A Randoux ◽  
J P Borel
Keyword(s):  
Superoxide Anion ◽  
Polymorphonuclear Neutrophils ◽  
Superoxide Anions ◽  
Collagen Concentration ◽  
Superoxide Anion Production ◽  
Chemiluminescence Analysis ◽  
Soluble Collagen ◽  
Acid Soluble Collagen ◽  
Anion Production ◽  
Calf Skin

Human polymorphonuclear neutrophils (PMNs), purified on Ficoll-Hypaque cushions, were incubated for 5 min with calf skin acid-soluble collagen and the released superoxide anions (O2-) measured spectrophotometrically by reduction of ferricytochrome c or by chemiluminescence analysis. This collagen stimulated the release of O2- unless it had been treated with pepsin. The stimulatory activity remained in denatured collagen, was contained only in the alpha 1(I) chain and was present in the alpha 1(I)-CB 6 (CNBr-cleaved) peptide, which is C-terminal. The activity was linearly dependent on the collagen concentration up to about 200 micrograms/ml. In addition, this collagen induced a release of beta-glucuronidase and N-acetyl-beta-glucosaminidase from PMNs.

scholarly journals Biochemical properties of polymorphonuclear neutrophils from venous blood and peritoneal exudates of rabbits

1980 ◽  
Vol 29 (2) ◽  
pp. 395-400
Author(s):  
K Takamori ◽  
T Yamashita
Keyword(s):  
Superoxide Anion ◽  
Venous Blood ◽  
Osmotic Fragility ◽  
Biochemical Properties ◽  
Polymorphonuclear Neutrophils ◽  
Superoxide Generation ◽  
Superoxide Anion Production ◽  
Anion Production ◽  
Blood Neutrophils ◽  
Bactericidal Activities

The biochemical properties of polymorphonuclear neutrophils from blood and peritoneal exudates of rabbits were compared. All enzymes measured showed almost identical activities in both types of cells, except for alkaline phosphodiesterase, the activity of which was seven times higher in peritoneal neutrophils. During phagocytosis, blood and peritoneal beta-glucuronidases were released in very similar fashions. Lysozyme, one of the enzymes concerned with killing of bacteria, as well as beta-glucuronidase, showed the same releasing pattern in both types of cells, but peroxidase was hardly released. Although superoxide anion generation in peritoneal neutrophils was two times higher than superoxide generation in blood neutrophils, phagocytic and bactericidal activities were almost the same in blood and peritoneal neutrophils. Blood neutrophils were more resistant to hypotonic lysis than were peritoneal neutrophils. These results show that there are no distinct differences in enzymatic and functional properties between blood and peritoneal polymorphonuclear neutrophils, except for alkaline phosphodiesterase activity, superoxide anion production, and osmotic fragility.

Lactate and P o 2 Modulate Superoxide Anion Production in Bovine Cardiac Myocytes

Circulation ◽  
1997 ◽  
Vol 96 (2) ◽  
pp. 614-620 ◽  
Author(s):  
Kamal M. Mohazzab-H. ◽  
Pawel M. Kaminski ◽  
Michael S. Wolin
Keyword(s):  
Cardiac Myocytes ◽  
Superoxide Anion ◽  
Superoxide Anion Production ◽  
Anion Production
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