Prolactin Concentrations in Follicular Fluid following Ovarian Hyperstimulation for in vitro Fertilization

1985 ◽  
Vol 22 (3) ◽  
pp. 189-195 ◽  
Author(s):  
H.G. Bohnet ◽  
V. Baukloh
Keyword(s):  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Ovarian Hyperstimulation ◽  
Vitro Fertilization

scholarly journals Serum and follicular fluid levels of sirtuin 1, sirtuin 6, and resveratrol in women undergoing in vitro fertilization: an observational, clinical study

2018 ◽  
Vol 47 (2) ◽  
pp. 772-782 ◽  
Author(s):  
József Bódis ◽  
Endre Sulyok ◽  
Tamás Kőszegi ◽  
Krisztina Gödöny ◽  
Viktória Prémusz ◽  
...  
Keyword(s):  
Clinical Study ◽  
In Vitro Fertilization ◽  
Pregnant Women ◽  
Follicular Fluid ◽  
Clinical Pregnancy ◽  
Sirtuin 1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ovarian Hyperstimulation ◽  
Vitro Fertilization

Objective This observational, clinical study was designed to assess the role of sirtuin 1 (SIRT1), sirtuin 6 (SIRT6), and resveratrol in in vitro fertilization (IVF). Methods Paired serum and follicular fluid (FF) samples were obtained from 30 consecutive patients (age: 36.43 ± 4.17 years, body mass index: 22.90 ± 2.05 kg/m2, duration of infertility: 5.10 ± 2.80 years) who received IVF treatment. SIRT1, SIRT6, and resveratrol levels were measured by enzyme-linked immunosorbent assay. Results Ovarian hyperstimulation resulted in significantly higher serum SIRT1 levels in pregnant women (8 patients) compared with non-pregnant women (22 patients). SIRT6 levels remained unchanged after ovarian hyperstimulation, but were significantly lower in pregnant women compared with non-pregnant women before and after hyperstimulation. Both SIRTs were detected in FF, but they appeared to be independent of their serum levels. After correction for confounders, FF SIRT6 levels were positively related to mature oocytes (F = 6.609), whereas serum SIRT1 and SIRT6 levels were related to clinical pregnancy (F = 10.008, F = 5.268, respectively). Conclusions Our study shows that SIRT1 and SIRT6, but not resveratrol, are involved in human reproduction and they may have a role in oocyte maturation and clinical pregnancy in IVF.

scholarly journals Effect of endometriosis on the protein expression pattern of follicular fluid from patients submitted to controlled ovarian hyperstimulation for in vitro fertilization

2010 ◽  
Vol 25 (7) ◽  
pp. 1755-1766 ◽  
Author(s):  
Edson Guimaraes Lo Turco ◽  
Gustavo Henrique Martins Ferreira Souza ◽  
Jerusa Simone Garcia ◽  
Christina Ramires Ferreira ◽  
Marcos Nogueira Eberlin ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Protein Expression ◽  
Expression Pattern ◽  
Follicular Fluid ◽  
Controlled Ovarian Hyperstimulation ◽  
Ovarian Hyperstimulation ◽  
Protein Expression Pattern ◽  
Vitro Fertilization

Proteomic Analysis of Follicular Fluid from Patients with Endometriosis Submitted to Controlled Ovarian Hyperstimulation for In Vitro Fertilization.

2009 ◽  
Vol 81 (Suppl_1) ◽  
pp. 185-185
Author(s):  
Edson Guimaraes Lo Turco ◽  
Paula H. C. Lopes ◽  
Maisa M. S. Sumikawa ◽  
Thiesa Butterby Soler ◽  
Ricardo Pimenta Bertolla ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Proteomic Analysis ◽  
Controlled Ovarian Hyperstimulation ◽  
Ovarian Hyperstimulation ◽  
Vitro Fertilization

Immunoreactive inhibin in human follicular fluid in an ovarian hyperstimulation programme for in vitro fertilization: correlations and different forms

1993 ◽  
Vol 128 (5) ◽  
pp. 433-438 ◽  
Author(s):  
Bärbel Reichardt ◽  
Gerhard Mehltretter ◽  
Karin Lechner ◽  
Hans K Rjosk ◽  
Otto A Müller ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Cell Function ◽  
Human Menopausal Gonadotropin ◽  
Gel Chromatography ◽  
Ovarian Hyperstimulation ◽  
Human Follicular Fluid ◽  
Vitro Fertilization ◽  
Follicular Maturation

The inhibin concentration in 131 samples of human follicular fluid obtained from 31 women undergoing ovarian hyperstimulation for in vitro fertilization was measured using specific double antibody radioimmunoassay. We used the synthetic 1-32-α-inhibin as standard and radioiodinated 1-32-Tyr-α-inhibin as tracer. Antibodies were raised in rabbits by immunization with the synthetic peptide. Estradiol and progesterone concentrations were measured using commercial radioimmunoassays. Results: The inhibin concentration correlated with the estradiol (r = 0.57, N = 88.p<0.0001) and progesterone (r=0.82, N=88. p<0.0001) concentrations in human follicular fluid. The dosage of human menopausal gonadotropin given to individual patients correlated with the average inhibin concentration measured in their follicles (r = 0.72, N = 23, p<0.0001). Similarly, the size of follicles correlated with their inhibin content (r = 0.75, N = 131, p<0.0001). Nineteen samples of human follicular fluid originating from follicles of different size and volume were examined using gel-chromatography. In each human follicular fluid the main form of inhibin (32 kDa) was recovered. In small follicles (3 ml) we found 12.8±9.1% (mean±sd) of the whole immunoreactive inhibin eluating in the area of V0 (≤80 kDa). In the larger follicles (4-7 ml), however, only 4.4±4.2% of this large inhibin form could be found. Conclusions: Our data confirm that human menopausal gonadotropin stimulates ovarian inhibin production. In addition to the estradiol and progesterone concentrations, the inhibin concentration may be an index of granulosa cell function and follicular maturation. The occurrence of large molecular weight forms of inhibin in small follicles remains unclear. They may represent large precursor molecules which are proteolytically cleaved in more mature follicles.

Sign in / Sign up

Export Citation Format

Share Document