Different Acute in vivo Effects of Bacterial Derived and Pituitary Growth Hormone Preparations on Plasma Levels of Glucagon, Insulin and Free Fatty Acids in Rabbits

1985 ◽  
Vol 21 (1) ◽  
pp. 10-18 ◽  
Author(s):  
Jørgen Knudtzon ◽  
Paul D. Edminson ◽  
Karl Ludvig Reichelt
Keyword(s):  
Fatty Acids ◽  
Growth Hormone ◽  
Free Fatty Acids ◽  
Plasma Levels ◽  
Pituitary Growth Hormone ◽  
In Vivo Effects

Acute in-vivo effects of adrenocorticotrophin on plasma levels of glucagon, insulin, glucose and free fatty acids in rabbits: involvement of the alpha-adrenergic nervous system

1984 ◽  
Vol 100 (3) ◽  
pp. 345-352 ◽  
Author(s):  
J. Knudtzon
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Plasma Levels ◽  
Intermediate Lobe ◽  
Blocking Drug ◽  
Alpha Adrenergic Receptors ◽  
Adrenergic Nervous System ◽  
In Vivo Effects ◽  
Adrenergic Blocking

ABSTRACT Intravenous injection of 8·5 nmol (1–24)ACTH increased the plasma levels of glucagon, insulin, glucose and free fatty acids in rabbits. The (1–24)ACTH-induced hyperglucagonaemia and hyperinsulinaemia started 3 and 20 min after the injection respectively. Similar increases in the plasma levels of glucagon, insulin and free fatty acids were found with 5·3 nmol (1–39)ACTH, whereas (1–4)ACTH, (4–10)ACTH, (1–10)ACTH, (11–24)ACTH, (7–38)ACTH and (18–39)ACTH (corticotrophin-like intermediate lobe peptide) injected at doses of approximately 8 nmol were inactive. Infusions with the alpha-adrenergic blocking drug, phentolamine, reduced the (1–24) ACTH-induced hyperglucagonaemia and hypergly-caemia, and augmented the (1–24)ACTH-induced hyperinsulinaemia, which now became significant after 5 min. Infusions with the beta-adrenergic blocking drug, propranolol, did not diminish the (1–24)ACTH-induced effects, but killed the rabbits after 2–4 h. It is concluded that the acute in-vivo effects of ACTH in rabbits are modulated by the involvement of alpha-adrenergic receptors, which increased the plasma levels of glucagon and glucose, and delayed and diminished the ACTH-induced increases in the plasma levels of insulin. The (1–24)ACTH-induced increases in the plasma levels of free fatty acids were not influenced by the adrenergic blocking drugs. J. Endocr. (1984) 100, 345–352

In Vivo Effects of Insulin and Free Fatty Acids on Matrix Metalloproteinases in Rat Aorta

Diabetes ◽  
2007 ◽  
Vol 57 (2) ◽  
pp. 476-483 ◽  
Author(s):  
G. Boden ◽  
W. Song ◽  
L. Pashko ◽  
K. Kresge
Keyword(s):  
Fatty Acids ◽  
Matrix Metalloproteinases ◽  
Free Fatty Acids ◽  
Rat Aorta ◽  
In Vivo Effects

Evidence for a Direct Pituitary Inhibition by Free Fatty Acids of in vivo Growth Hormone Responses to Growth Hormone-Releasing Hormone in the Rat

1991 ◽  
Vol 53 (2) ◽  
pp. 185-189 ◽  
Author(s):  
Clara V. Alvarez ◽  
Federico Mallo ◽  
Bartolome Burguera ◽  
Luanda Cacicedo ◽  
Carlos Dieguez ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Growth Hormone ◽  
Free Fatty Acids ◽  
Growth Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Hormone Responses ◽  
In Vivo Growth

In vitro and in vivo effects of increased concentrations of free fatty acids on free thyroxin measurements as determined by five assays

1990 ◽  
Vol 36 (4) ◽  
pp. 611-613 ◽  
Author(s):  
R Sapin ◽  
J L Schlienger ◽  
F Grunenberger ◽  
F Gasser ◽  
J Chambron
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
The Other ◽  
In Vitro Experiments ◽  
Fat Emulsion ◽  
Before And After ◽  
High Concentrations ◽  
In Vivo Effects

Abstract To compare in vitro and in vivo effects of increased concentrations of free fatty acids (FFA) on free thyroxin (FT4) values, we measured FT4 in three pooled sera supplemented with oleate and in serum from 18 euthyroid patients before and after an infusion of fat emulsion (Intralipid). We used five FT4 RIA kits: two two-step methods [Gammacoat, Baxter (GC); Ria-gnost, Behring (RG)], two analog RIAs [Amerlex-M, Amersham (AM); Coat-Ria, BioMérieux (CR)], and one kit with labeled antibodies [Amerlex-MAB*, Amersham (AA)]. In vitro, at the maximum oleate addition of 5 mmol/L, FT4 increased when measured by the GC and RG kits, decreased by the AM kit, and showed no significant change by the CR and AA kits. In vivo, post-Intralipid, FFA concentrations rose significantly and the FT4 changes agreed with the results of the in vitro experiments, except for the RG kit, for which FT4 increased in only nine patients. We conclude that in vitro oleate addition is useful to predict the in vivo effect of increased FFA on FT4 values; moreover, in serum from euthyroid subjects with high concentrations of FFA, FT4 analyzed with the CR or AA kits should better agree with normal results for thyrotropin than FT4 values measured with the other kits.

Utilization of free fatty acids by diaphragm in vitro

1962 ◽  
Vol 203 (2) ◽  
pp. 311-315 ◽  
Author(s):  
P. T. Bodel ◽  
D. Rubinstein ◽  
E. E. McGarry ◽  
J. C. Beck
Keyword(s):  
Fatty Acids ◽  
Growth Hormone ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acids ◽  
Gastrocnemius Muscle ◽  
Rat Diaphragm ◽  
Diaphragm In Vitro

Human and rat diaphragm and rat gastrocnemius muscle oxidize and esterify palmitate-1-C14. Incorporation of C14 was found to be in proportion to the palmitate present in the medium over a range of concentrations from 0.8 to 2.9 µEq/ml. Fasting increased C14O2 production but had no effect on esterification. Iodoacetate or an atmosphere of nitrogen inhibited esterification of palmitate but increased the amount of free fatty acid in the tissue. Insulin increased esterification, and this was enhanced by the addition of glucose. Glucose and insulin exerted a sparing action on the oxidation of glycerides by a tissue previously charged with palmitate-1-C14. Growth hormone administered in vivo over a period of 1 week or in vitro during incubation had no effect on esterification or metabolism of palmitate-1-C14.

scholarly journals Effect of changes in plasma levels of free fatty acids on plasma glucagon, insulin, and growth hormone in man

Metabolism ◽  
1977 ◽  
Vol 26 (9) ◽  
pp. 1011-1023 ◽  
Author(s):  
Brendan H. Hicks ◽  
Charles I. Taylor ◽  
Satish K. Vij ◽  
Sumer Pek ◽  
Ralph F. Knopf ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Growth Hormone ◽  
Free Fatty Acids ◽  
Plasma Levels ◽  
Plasma Glucagon
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