Radioimmunoassays for Human Chorionic Gonadotrophin and Human Hypophysial LH Using the Double Antibody Method

1974 ◽  
Vol 5 (6) ◽  
pp. 367-375 ◽  
Author(s):  
H.E. Hildebrandt-Stark ◽  
M. Vekemans ◽  
C. Robyn
Keyword(s):  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Double Antibody ◽  
Antibody Method

THE PRECISION OF THE OVARIAN ASCORBIC ACID DEPLETION TEST FOR LUTEINIZING HORMONE

1963 ◽  
Vol 43 (1) ◽  
pp. 155-160
Author(s):  
Jørgen Falck Larsen ◽  
Christian Hamburger
Keyword(s):  
Ascorbic Acid ◽  
Luteinizing Hormone ◽  
Clinical Analysis ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin

ABSTRACT Various modifications of the Parlow test for luteinizing hormone (ovarian ascorbic acid depletion in rats) were tried. Human chorionic gonadotrophin was used instead of hypophyseal luteinizing hormone. The precision of the method was found to be so low, however, that the test could not be used for routine clinical analysis. The low precision found in this and other laboratories is thought to be due to the strains of rats used.

STUDIES ON THE AROMATISATION OF NEUTRAL STEROIDS IN PREGNANT WOMEN

1964 ◽  
Vol 45 (4) ◽  
pp. 535-559 ◽  
Author(s):  
E. Bolté ◽  
S. Mancuso ◽  
G. Eriksson ◽  
N. Wiqvist ◽  
E. Diczfalusy
Keyword(s):  
Pregnant Women ◽  
Comparative Studies ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Radioactive Material ◽  
Placental Barrier ◽  
Therapeutic Abortion ◽  
Limited Ability ◽  
Better Than

ABSTRACT In 15 cases of therapeutic abortion by laparotomy the placenta was disconnected from the foetus and perfused in situ with tracer amounts of radioactive dehydroepiandrosterone (DHA), dehydroepiandrosterone sulphate (DHAS), androst-4-ene-3,17-dione (A), testosterone (T) and 17β-oestradiol (OE2). Analysis of the placentas, perfusates and urine samples revealed an extensive aromatisation of DHA, A and T; more than 70% of the radioactive material recovered was phenolic, and at least 80 % of this phenolic material was identified as oestrone (OE1), 17β-oestradiol (OE2) and oestriol (OE3), the latter being detected only in the urine. Comparative studies indicated that A and T were aromatised somewhat better than DHA and that all three unconjugated steroids were aromatised to a much greater extent than DHAS. Radioactive OE1 and OE2 were isolated and identified in the placentas and perfusates, but no OE3, epimeric oestriols, or ring D ketols could be detected in these sources, not even when human chorionic gonadotrophin (HCG) was added to the blood prior to perfusion. Lack of placental 16-hydroxylation was also apparent when OE2 was perfused. Regardless of the precursor perfused, there was three times more OE2 than OE1 in the placenta and three times more OE1 than OE2 in the perfusate. This was also the case following perfusion with OE2. The results are interpreted as suggesting the existence in the pregnant human of a placental »barrier« limiting the passage of circulating androgen. The barrier consists of a) limited ability to transfer directly DHAS and b) an enzymic mechanism resulting in the rapid and extensive aromatisation of the important androgens DHA, A and T.

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