Isolation and Characterization of a Microvascular Fraction from Rabbit Kidney Cortex

1984 ◽  
Vol 7 (3) ◽  
pp. 146-155 ◽  
Author(s):  
J.J. Helwig ◽  
C. Judes ◽  
C. Bollack ◽  
P. Mandel
Keyword(s):  
Rabbit Kidney ◽  
Kidney Cortex ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of rabbit kidney brush borders

1972 ◽  
Vol 128 (5) ◽  
pp. 1319-1328 ◽  
Author(s):  
S. J. Quirk ◽  
G. B. Robinson
Keyword(s):  
Plasma Membranes ◽  
Sucrose Density Gradient ◽  
Molar Ratio ◽  
Rabbit Kidney ◽  
Kidney Cortex ◽  
Marker Enzymes ◽  
Isolation And Characterization ◽  
Adenosine Triphosphatases ◽  
Brush Borders

1. Brush borders were isolated from rabbit kidney-cortex homogenates by rate-zonal centrifugation through a sucrose density gradient in a B-XIV zonal rotor, followed by differential centrifugation. 2. The method of preparation gave brush borders of high purity with a reasonable yield. The morphological appearance supported the evidence from enzymic and chemical investigations, that the brush borders were only slightly contaminated with endoplasmic reticulum, mitochondria, lysosomes and nuclei. 3. The molar ratio of cholesterol to phospholipid lay within the range found in other plasma membranes, but the carbohydrate content was double that found in liver plasma membranes. 4. Alkaline phosphatase, maltase, trehalase and aminopeptidase were major enzymic constituents of the brush borders, and had an approximately equal yield and enrichment, but none of these enzymes fulfilled the criteria for marker enzymes. 5. Mg2+-dependent and Na+,K+-dependent adenosine triphosphatases, although found in brush borders, had low yields and low enrichments.

Binding of peanut lectin to specific epithelial cell types in kidney

1982 ◽  
Vol 242 (1) ◽  
pp. C117-C120 ◽  
Author(s):  
M. LeHir ◽  
B. Kaissling ◽  
B. M. Koeppen ◽  
J. B. Wade
Keyword(s):  
Cell Types ◽  
Rabbit Kidney ◽  
Specific Cell ◽  
Luminal Membrane ◽  
Isolation And Characterization ◽  
Collecting Ducts ◽  
Epithelial Membranes ◽  
Outer Stripe ◽  
Specific Affinity

The binding of peanut agglutinin (PNA) to epithelial membranes of the rabbit kidney was evaluated at the light- and electron-microscope level using PNA conjugated to horseradish peroxidase. In the renal cortex and outer stripe of the medulla PNA appears to bind exclusively to the luminal membrane of intercalated cells in connecting tubules and collecting ducts. PNA also binds to the thin descending limb of the loop of Henle in the inner stripe and inner zone of the medulla. This very specific affinity of PNA should be useful in the isolation and characterization of specific cell types in cytologically heterogeneous epithelia.

Purification and Characterization of Two Forms of Fatty Acid ω-Hydroxylase Cytochrome P-450 from Rabbit Kidney Cortex Microsomes1

1990 ◽  
Vol 108 (4) ◽  
pp. 544-548 ◽  
Author(s):  
Rikio Yoshimura ◽  
Emi Kusunose ◽  
Noboru Yokotani ◽  
Satoru Yamamoto ◽  
Ichiro Kubota ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Rabbit Kidney ◽  
Kidney Cortex ◽  
Purification And Characterization ◽  
Cytochrome P 450

Isolation and characterization of dipeptidyl aminopeptidase IV from human kidney cortex

1989 ◽  
Vol 179 (1) ◽  
pp. 61-71 ◽  
Author(s):  
Gunter B. Wolf ◽  
Jürgen E. Scherberich ◽  
Peter Fischer ◽  
Wilhelm Schoeppe
Keyword(s):  
Human Kidney ◽  
Kidney Cortex ◽  
Isolation And Characterization ◽  
Dipeptidyl Aminopeptidase
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