Impaired Response to Vasopressin of Adenylate Cyclase of the Thick Ascending Limb of Henle’s Loop in Brattleboro Rats with Diabetes insipidus

1978 ◽  
Vol 1 (1) ◽  
pp. 3-10 ◽  
Author(s):  
Martine Imbert-Teboul ◽  
Danielle Chabardès ◽  
Madeleine Montégut ◽  
André Clique ◽  
François Morel
Keyword(s):  
Adenylate Cyclase ◽  
Diabetes Insipidus ◽  
Thick Ascending Limb ◽  
Brattleboro Rats

Influence of chronic ADH treatment on adenylate cyclase and ATPase activity in distal nephron segments of diabetes insipidus Brattleboro rats

1985 ◽  
Vol 405 (3) ◽  
pp. 216-222 ◽  
Author(s):  
M. M. Trinh-Trang-Tan ◽  
L. Bankir ◽  
A. Doucet ◽  
G. El Mernissi ◽  
M. Imbert-Teboul ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Atpase Activity ◽  
Diabetes Insipidus ◽  
Brattleboro Rats ◽  
Distal Nephron ◽  
Nephron Segments

Antidiuretic antagonism and agonism of 1-deamino-pentamethylene-2-d-phenylalanine-4-isoleucine-arginine vasopressin in rats with diabetes insipidus

1987 ◽  
Vol 112 (3) ◽  
pp. 439-442 ◽  
Author(s):  
H. Vilhardt ◽  
S. Lundin
Keyword(s):  
Diabetes Insipidus ◽  
Arginine Vasopressin ◽  
Test Model ◽  
Brattleboro Rats ◽  
Experimental Conditions ◽  
Vasopressin Antagonist ◽  
Agonist Action

ABSTRACT Using implanted minipumps it was shown over a period of 7 days that the vasopressin antagonist, 1-deamino-pentamethylene-2-d-Phe-4-Ile-arginine vasopressin, caused increased diuresis in normal rats and reversed vasopressin- or oxytocin-induced antidiuresis in Brattleboro rats. When the antagonist was infused alone in Brattleboro rats it induced a marked antidiuretic response, indicating that the analogue also possessed agonistic properties. The agonist action could not be demonstrated in anaesthetized, hydrated normal rats. In these animals the analogue behaved as a pure antagonist. It is concluded that analogues which behave as antagonists in one test model may display agonistic properties under different experimental conditions. J. Endocr. (1987) 112, 439–442

Effects of dDAVP on rat juxtamedullary nephrons: stimulation of medullary K recycling

1985 ◽  
Vol 249 (2) ◽  
pp. F291-F298 ◽  
Author(s):  
J. M. Elalouf ◽  
N. Roinel ◽  
C. de Rouffignac
Keyword(s):  
Parathyroid Hormone ◽  
Adenylate Cyclase ◽  
Excretion Rate ◽  
Distal Tubule ◽  
Fractional Excretion ◽  
The Other ◽  
Thick Ascending Limb ◽  
Adenylate Cyclase System ◽  
Circulating Levels ◽  
Stimulation Of

The effects of 1-desamino-8-D-arginine vasopressin (dDAVP) on the handling of water and electrolytes by the juxtamedullary nephrons were studied on rats with reduced circulating levels of antidiuretic hormone (ADH), parathyroid hormone, calcitonin, and glucagon, all of which stimulate the adenylate cyclase system of the thick ascending limb and the distal tubule. In such hormone-deprived rats and in hormone-deprived + dDAVP rats, the concentration of Na, Cl, and total solutes was lower in the ascending than in the descending limbs, whereas the inulin concentration was similar at both sites. dDAVP did not alter the fraction of NaCl remaining in the thin limbs, but tended to reduce that of Mg and Ca. On the other hand, dDAVP significantly increased the fraction of filtered K remaining from 65.8 +/- 5.2 to 107.3 +/- 15.8%. A direct correlation was observed between the fraction of filtered K remaining at the tip of the juxtamedullary loops and the fractional excretion rate of K in urine. Since dDAVP enhances distal K net secretion, as previously shown in our laboratory, these results indicate that the medullary recycling of K from nephron terminal segments to Henle's loop of juxtamedullary nephrons is stimulated by this peptide.

Role of arginine vasopressin in medullary thick ascending limb on maximal urinary concentration

1986 ◽  
Vol 251 (2) ◽  
pp. F266-F270 ◽  
Author(s):  
J. K. Kim ◽  
S. N. Summer ◽  
A. E. Erickson ◽  
R. W. Schrier
Keyword(s):  
Adenylate Cyclase ◽  
Arginine Vasopressin ◽  
Urinary Concentration ◽  
Thick Ascending Limb ◽  
Sprague Dawley ◽  
Urinary Osmolality ◽  
Medullary Thick Ascending Limb ◽  
Sprague Dawley Rats ◽  
Collecting Tubules

Two groups of Sprague-Dawley rats, Harlan (H) and Charles River (CR), were discovered in that the medullary thick ascending limb (MAL) had a profoundly different adenylate cyclase response to arginine vasopressin (AVP). Using these two groups of rats, we studied the correlation between AVP action on the MAL and maximal urinary concentration. AVP (10(-6) M) significantly stimulated adenylate cyclase in MAL of H rats (7.4 +/- 0.9 to 43.8 +/- 4.6 fmol cAMP formed X 30 min-1 X mm-1, P less than 0.001) but not in CR rats (10.3 +/- 1.4 to 12.7 +/- 2.0 fmol cAMP formed X 30 min-1 X mm-1, NS). In contrast, AVP significantly stimulated adenylate cyclase of cortical, outer and inner medullary collecting tubules from both H and CR rats. Glucagon (10(-6) M) significantly stimulated adenylate cyclase of MAL from both H and CR rats. After 48 h of fluid deprivation, urinary osmolality was significantly higher (P less than 0.001) in the H (4,504 +/- 399 mosmol/kg H2O, n = 14) than CR (2,840 +/- 176 mosmol/kg H2O, n = rats. This observation was not attributable to differences in creatinine clearance (CR, 1.30 +/- 0.24; H, 1.24 +/- 0.03 ml/min, NS, n = 4) or plasma AVP (CR, 12.75 +/- 1.44; H, 12.38 +/- 1.17 pg/ml, NS, n = 6) levels. These results therefore suggest that the action of AVP on the MAL, in addition to the effect on collecting tubules, is involved in maximal urinary concentration in rats.

NaCl and Ca delivery at the bend of rat deep nephrons decreases during antidiuresis

1987 ◽  
Vol 252 (6) ◽  
pp. F1055-F1064 ◽  
Author(s):  
J. M. Elalouf ◽  
D. Chabane Sari ◽  
N. Roinel ◽  
C. de Rouffignac
Keyword(s):  
Parathyroid Hormone ◽  
Diabetes Insipidus ◽  
Arginine Vasopressin ◽  
Distal Tubule ◽  
Water Removal ◽  
Loop Of Henle ◽  
Brattleboro Rats ◽  
Previous Hypothesis

The effects of 1-desamino-8-D-arginine vasopressin (dDAVP) on superficial and juxtamedullary nephrons were investigated by micropuncture in diabetes insipidus (DI) Brattleboro rats chronically treated with the peptide. The rats, acutely deprived of endogenous calcitonin, parathyroid hormone (PTH), and glucagon [hormone-deprived (HD) rats], were examined either 4 days after cessation of dDAVP treatment (HDT, control diuretic rats) or when the treatment was continued until the micropuncture experiment, during which dDAVP was also given intravenously (HDT + dDAVP, experimental nondiuretic rats). In the presence of dDAVP, the reabsorption of Cl, Na, Mg, and Ca by the superficial loop of Henle was significantly increased, as previously observed in HD-untreated rats during acute infusion of dDAVP. The effects on the superficial distal tubule were also similar. The effects on K, however, were different both in the loop and in the distal tubule. At the bend of the juxtamedullary nephrons, the treatment alone (HDT rats) increased fractional delivery (FD%) of Na and Cl, whereas FD% of Mg, Ca, K, and P was unaltered. In HDT + dDAVP rats, FD% of H2O, Cl, Na, and Ca was significantly lower than in HDT rats, and FD% of K, Mg, and P did not differ significantly. In conclusion, in the presence of dDAVP, the FD% of H2O, Na, and Cl at the bend of the long-loop nephrons decreases, in accordance with our previous hypothesis that water removal along the rat descending limb increases outward NaCl diffusion along this segment.

Dynamics of nucleotides in distal nephron of mice with nephrogenic diabetes insipidus

1986 ◽  
Vol 250 (1) ◽  
pp. F151-F158 ◽  
Author(s):  
E. Kusano ◽  
A. N. Yusufi ◽  
N. Murayama ◽  
J. Braun-Werness ◽  
T. P. Dousa
Keyword(s):  
Diabetes Insipidus ◽  
Nephrogenic Diabetes Insipidus ◽  
Fold Increase ◽  
Thick Ascending Limb ◽  
Camp Phosphodiesterase ◽  
Camp Content ◽  
Medullary Thick Ascending Limb ◽  
Atp Levels ◽  
Collecting Tubules ◽  
Camp Levels

In mice with hereditary nephrogenic diabetes insipidus (NDI), the high activity of cAMP-phosphodiesterase (cAMP-PDIE) in medullary collecting tubules (MCT) prevents the increase in cAMP content in response to vasopressin [Arg8]vasopressin (AVP). Even when the cAMP response to AVP is partly corrected by cAMP-PDIE inhibitor 1-methyl-3-isobutylxanthine (MIX), under all tested conditions the cAMP levels in MCT of NDI mice remained much lower than in controls (B. A. Jackson, R. M. Edwards, H. Valtin, and T. P. Dousa, J. Clin. Invest. 66: 110-122, 1980). In the present study, we explored which factors may account for this defect. We determined contents of ATP, nicotinamide adenine dinucleotide (NAD), and the levels of cAMP in MCT and in medullary thick ascending limb of Henle's loop (MAL) microdissected from control and NDI mice. In the presence of 1 microM AVP and 0.05 mM MIX, the cAMP levels accumulated in MCT of NDI mice were four times lower compared with controls, but the levels of ATP and NAD were not different. ATP levels in MAL of NDI mice were slightly (delta -23%) lower than in MAL from controls, and in distal convoluted tubules (DCT) of NDI mice the ATP levels were also decreased (delta -49%). Although AVP alone had little effect on cAMP levels in mouse MAL in the presence of 0.1 mM forskolin, the AVP elicited a 20-fold increase of cAMP of both the control and NDI mice. Addition of 0.1 mM forskolin further increased the cAMP accumulation in MCT incubated with AVP.(ABSTRACT TRUNCATED AT 250 WORDS)

High, vasopressin-reversible, immunoreactive somatostatin in specific hypothalamic nuclei of rats with diabetes insipidus (Brattleboro rats)

1983 ◽  
Vol 277 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Juan M. Saavedra ◽  
Catherine Rougeot ◽  
Claude Chevillard ◽  
Fernand Dray
Keyword(s):  
Diabetes Insipidus ◽  
Brattleboro Rats ◽  
Hypothalamic Nuclei
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