Induction of Syncytia by Simian Sarcoma Virus Type I (SSV-I/SSAV-I) in Several Human Transformed Cell Lines

Pathobiology ◽  
1980 ◽  
Vol 48 (6) ◽  
pp. 421-428
Author(s):  
M. Ocho ◽  
H. Ogura ◽  
T. Tanaka ◽  
T. Oda
Keyword(s):  
Cell Lines ◽  
Virus Type ◽  
Type I ◽  
Transformed Cell ◽  
Sarcoma Virus ◽  
Simian Sarcoma Virus ◽  
Transformed Cell Lines

scholarly journals Selective killing of human T cell lymphotropic virus type I-transformed cell lines by a damavaricin Fc derivative.

1989 ◽  
Vol 42 (5) ◽  
pp. 779-787 ◽  
Author(s):  
SHIN-ICHI ITO ◽  
NAOKI YAMAMOTO ◽  
KIKUO NOMOTO ◽  
KAZUYA SASAKI ◽  
KAZUKIYO ONODERA
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
Virus Type ◽  
Type I ◽  
Transformed Cell ◽  
Human T Cell ◽  
Transformed Cell Lines

Establishment of simian sarcoma virus, type 1 (SSV-1)-transformed non-producer marmoset cell lines

1977 ◽  
Vol 20 (1) ◽  
pp. 104-111 ◽  
Author(s):  
Carolyn M. Bergholz ◽  
Lauren G. Wolfe ◽  
Friedrich Deinhardt
Keyword(s):  
Cell Lines ◽  
Virus Type ◽  
Sarcoma Virus ◽  
Simian Sarcoma Virus

scholarly journals Inhibition of p53 Transactivation Function by the Human T-Cell Lymphotropic Virus Type 1 Tax Protein

1998 ◽  
Vol 72 (2) ◽  
pp. 1165-1170 ◽  
Author(s):  
Cynthia A. Pise-Masison ◽  
Kyeong-Sook Choi ◽  
Michael Radonovich ◽  
Jürgen Dittmer ◽  
Seong-Jin Kim ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
Virus Type ◽  
Primary Role ◽  
Wild Type ◽  
Transformed Cell ◽  
Human T Cell ◽  
Wild Type P53 ◽  
Transformed Cell Lines

ABSTRACT Human T-cell lymphotropic virus type 1 (HTLV-1) is the etiologic agent for adult T-cell leukemia. HTLV-1 transforms lymphocytes, and there is increasing evidence that the virus-encoded protein, Tax, plays a primary role in viral transformation. We have shown that wild-type p53 in HTLV-1-transformed cells is stabilized. This study was initiated to directly analyze whether the p53 in HTLV-1-transformed cell lines was transcriptionally active and to identify the viral gene product responsible for stabilization and inactivation. Transfection experiments using a p53-responsive reporter plasmid and γ-irradiation studies demonstrate that the wild-type p53 in HTLV-1-transformed cell lines is not fully active. Further, we demonstrate that the HTLV-1-transforming protein, Tax, stabilizes and inactivates p53 function. Cotransfection of Tax with p53 results in a greater than 10-fold reduction in p53 transcription activity. Using Gal4-p53 fusion proteins, we demonstrate that Tax inhibition of p53 transactivation function is independent of sequence-specific DNA binding. Moreover, Tax inhibits p53 function by interfering with the activity of the N-terminal activation domain (amino acids 1 to 52). We conclude that Tax is involved in the inactivation of p53 function and stabilization of p53 in HTLV-1-infected cells. The functional interference of p53 function by Tax may be important for transformation and leukemogenesis.

scholarly journals Integration and expression of several molecular forms of Rous sarcoma virus DNA used for transfection of mouse cells.

1984 ◽  
Vol 4 (7) ◽  
pp. 1260-1269 ◽  
Author(s):  
P A Luciw ◽  
H Oppermann ◽  
J M Bishop ◽  
H E Varmus
Keyword(s):  
Cell Lines ◽  
Rous Sarcoma Virus ◽  
Linear Molecule ◽  
Viral Dna ◽  
Transformed Cell ◽  
Rous Sarcoma ◽  
Src Gene ◽  
Sarcoma Virus ◽  
Mouse Cells ◽  
Transformed Cell Lines

To assess the factors required for integration and expression of retroviral DNA, we have examined viral DNA, RNA, and protein in NIH/3T3 mouse cells transformed by transfection with various forms of cloned Rous sarcoma virus (RSV) DNA. Linear RSV DNA molecules, derived from circular DNA containing two long terminal repeats (LTRs) and permuted by cleavage at the SacI restriction endonuclease site in the leader sequence, were integrated near the ends of the linear molecule, with the LTRs on the 3' side of the src gene. Integration of a subgenomic RSV DNA fragment containing the viral src gene without intact LTRs also occurred near the ends of the linear molecule. Head-to-tail tandem arrays of RSV DNA species were observed in some transformed cell lines that received fully digested DNA and in all cell lines that received DNA ligated to produce oligomers before transfection. Closed circular RSV DNA, with one or two LTRs, integrated without apparent specificity within several regions of the viral genome. After transfection with SacI-permuted RSV DNA still linked to arms of the lambda bacteriophage vector DNA, bacteriophage sequences were joined to host DNA. Transformed cell lines produced by transfection with the various forms of RSV DNA produced similar levels of viral src protein, although the efficiency of successful transformation varied by at least two orders of magnitude. Analyses of viral polyadenylated RNA, together with the patterns of viral DNA in transformed cells, indicated that viral DNA can be integrated and expressed without regard to LTR sequences, with adjacent host DNA presumably supplying signals required for the promotion and processing of functional src mRNA.

Integration and expression of several molecular forms of Rous sarcoma virus DNA used for transfection of mouse cells

1984 ◽  
Vol 4 (7) ◽  
pp. 1260-1269
Author(s):  
P A Luciw ◽  
H Oppermann ◽  
J M Bishop ◽  
H E Varmus
Keyword(s):  
Cell Lines ◽  
Rous Sarcoma Virus ◽  
Linear Molecule ◽  
Viral Dna ◽  
Transformed Cell ◽  
Rous Sarcoma ◽  
Src Gene ◽  
Sarcoma Virus ◽  
Mouse Cells ◽  
Transformed Cell Lines

To assess the factors required for integration and expression of retroviral DNA, we have examined viral DNA, RNA, and protein in NIH/3T3 mouse cells transformed by transfection with various forms of cloned Rous sarcoma virus (RSV) DNA. Linear RSV DNA molecules, derived from circular DNA containing two long terminal repeats (LTRs) and permuted by cleavage at the SacI restriction endonuclease site in the leader sequence, were integrated near the ends of the linear molecule, with the LTRs on the 3' side of the src gene. Integration of a subgenomic RSV DNA fragment containing the viral src gene without intact LTRs also occurred near the ends of the linear molecule. Head-to-tail tandem arrays of RSV DNA species were observed in some transformed cell lines that received fully digested DNA and in all cell lines that received DNA ligated to produce oligomers before transfection. Closed circular RSV DNA, with one or two LTRs, integrated without apparent specificity within several regions of the viral genome. After transfection with SacI-permuted RSV DNA still linked to arms of the lambda bacteriophage vector DNA, bacteriophage sequences were joined to host DNA. Transformed cell lines produced by transfection with the various forms of RSV DNA produced similar levels of viral src protein, although the efficiency of successful transformation varied by at least two orders of magnitude. Analyses of viral polyadenylated RNA, together with the patterns of viral DNA in transformed cells, indicated that viral DNA can be integrated and expressed without regard to LTR sequences, with adjacent host DNA presumably supplying signals required for the promotion and processing of functional src mRNA.

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