scholarly journals Mechanism of Cd-Induced Inhibition of Na-Glucose Cotransporter in Rabbit Proximal Tubule Cells: Roles of Luminal pH and Membrane-Bound Carbonic Anhydrase

2008 ◽  
Vol 110 (2) ◽  
pp. p11-p20 ◽  
Author(s):  
Shuichi Tsuruoka ◽  
Erik R. Swenson ◽  
Akio Fujimura ◽  
Masashi Imai
Keyword(s):  
Carbonic Anhydrase ◽  
Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Membrane Bound ◽  
Tubule Cells ◽  
Luminal Ph

Aminopeptidase N reduces basolateral Na+-K+-ATPase in proximal tubule cells

2007 ◽  
Vol 293 (4) ◽  
pp. F1047-F1053 ◽  
Author(s):  
Kumar Kotlo ◽  
Sagar Shukla ◽  
Urmila Tawar ◽  
Randal A. Skidgel ◽  
Robert S. Danziger
Keyword(s):  
Atpase Activity ◽  
Proximal Tubule ◽  
Aminopeptidase N ◽  
Angiotensin Iv ◽  
Proximal Tubule Cells ◽  
Renal Adaptation ◽  
Membrane Bound ◽  
Tubule Cells ◽  
Trait Locus ◽  
Ang Iv

Aminopeptidase N/CD13 (Anpep) is a membrane-bound protein that catalyzes the formation of natriuretic hexapeptide angiotensin IV (ANG IV) from ANG III. We previously reported that Anpep is more highly expressed in the kidneys of Dahl salt-resistant (SR/Jr) than salt-sensitive (SS/Jr) rats, Anpep maps to a quantitative trait locus for hypertension, and that the Dahl SR/Jr rat contains a functional polymorphism of the gene. This suggests that renal Anpep may be linked to salt sensitivity; however, its effect on renal Na handling has not been determined. Here, we examined regulation of basolateral Na+-K+-ATPase, a preeminent basolateral Na+ transporter in proximal tubule cells, by Anpep in LLC-PK1 cells. Treatment of the cells with Anpep siRNA increased total cellular Na+-K+-ATPase activity and basolateral Na+-K+-ATPase abundance by approximately twofold. Conversely, Anpep overexpression reduced Na+-K+-ATPase activity and basolateral abundance by ∼50%. Similar effects were observed after treatment with ANG IV (10 nM, ×30 min and 12 h). ANG IV receptor (AGTRIV) knockdown via specific siRNA relieved the decreases in basolateral Na+-K+-ATPase levels and activity induced by Anpep overexpression. In sum, these results demonstrate that Anpep reduces basolateral Na+-K+-ATPase levels via ANG IV/AGTRIV signaling. This novel pathway may be important in renal adaptation to high salt.

scholarly journals Molecular mechanism of kNBC1-carbonic anhydrase II interaction in proximal tubule cells

2004 ◽  
Vol 559 (1) ◽  
pp. 55-65 ◽  
Author(s):  
Alexander Pushkin ◽  
Natalia Abuladze ◽  
Eitan Gross ◽  
Debra Newman ◽  
Sergei Tatishchev ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Proximal Tubule ◽  
Molecular Mechanism ◽  
Carbonic Anhydrase Ii ◽  
Proximal Tubule Cells ◽  
Tubule Cells

scholarly journals Immortalized rat proximal tubule cells produce membrane bound and soluble megalin

1998 ◽  
Vol 53 (2) ◽  
pp. 358-366 ◽  
Author(s):  
Flavia F. Jung ◽  
David R. Bachinsky ◽  
Shiow-Shih Tang ◽  
Gang Zheng ◽  
Daniel Diamant ◽  
...  
Keyword(s):  
Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Membrane Bound ◽  
Tubule Cells ◽  
Rat Proximal Tubule

scholarly journals Histochemical detection of carbonic anhydrase with diemthylaminonaphthalene-5-sulfonamide.

1979 ◽  
Vol 27 (7) ◽  
pp. 1103-1107 ◽  
Author(s):  
C Pochhammer ◽  
P Dietsch ◽  
P R Siegmund
Keyword(s):  
Carbonic Anhydrase ◽  
Proximal Tubule ◽  
Specific Method ◽  
Proximal Tubule Cells ◽  
Histochemical Detection ◽  
Tubule Cells ◽  
The Difference

A new specific method for the detection of carbonic anhydrase, EC 4.2.1.1, in tissues is described. The reaction of carbonic anhydrase with dimethylaminonaphthalene-5-sulfonamide (DNSA) forms a highly fluorescent complex. The specificity of the method is proved by the quenching of this fluorescence with ethoxzolamide (6-ethoxybenzothiazole-5-sulfonamide). The difference in the wavelength makes it possible to absorb the fluorescence of the unbound dimethylaminonaphthalene-5-sulfonamide by filters. Kidney, proventriculus, and bone from chicken have been examined. Carbonic anhydrase has been detected in the cytoplasm of the columnar lining cells, proximal tubule cells, and osteoclasts.

Nephrotoxicity Testing In Vitro: The Current Situation

1997 ◽  
Vol 25 (5) ◽  
pp. 497-503
Author(s):  
Jean-Paul Morin ◽  
Marc E. De Broe ◽  
Walter Pfaller ◽  
Gabriele Schmuck
Keyword(s):  
Proximal Tubule ◽  
Culture Media ◽  
Task Force ◽  
Primary Cultures ◽  
Phenotypic Expression ◽  
Transepithelial Transport ◽  
Specific Cell ◽  
Proximal Tubule Cells ◽  
Tubule Cells

An ECVAM task force on nephrotoxicity has been established to advise, in particular, on the follow-up to recommendations made in the ECVAM workshop report on nephrotoxicity testing in vitro. Since this workshop was held, in 1994, there have been several improvements in the techniques used. For example, the duration of renal slice viability, and the maintenance of functional activities in slices, have been improved by using dynamic incubation systems with higher oxygen tensions and more-appropriate cell culture media. Highly differentiated primary cultures of pig, human and rabbit proximal tubule cells have been established by using specific cell isolation procedures and/or selective culture media. To date, the most comparable phenotypic expression and transepithelial transport capacities to proximal tubules in vivo have been obtained with primary cultures of rabbit proximal tubule cells which are grown on bicompartmental supports; in this system, transepithelial substrate gradients are generated and the transepithelial transport of both organic anions and cations is highly active. This in vitro system has been selected by ECVAM for further evaluation and prevalidation. Industrial needs in the area of nephrotoxicity testing have been identified, and recommendations are made at the end of this report concerning possible future initiatives.

Effects of TCDD and estradiol-17β on the proliferation and Na+/glucose cotransporter in renal proximal tubule cells

2005 ◽  
Vol 19 (1) ◽  
pp. 21-30 ◽  
Author(s):  
Ho Jae Han ◽  
Min Jin Lim ◽  
Yun Jung Lee ◽  
Eun Jung Kim ◽  
Young Jin Jeon ◽  
...  
Keyword(s):  
Proximal Tubule ◽  
Renal Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Renal Proximal Tubule Cells ◽  
Estradiol 17Β
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