Evaluation of the Fluorescent Antibody Technique for the Detection of Rocky Mountain Spotted Fever Rickettsiae in Various Tissues

Pathobiology ◽  
1961 ◽  
Vol 24 (1) ◽  
pp. 27-39 ◽  
Author(s):  
W. Burgdorfer
Keyword(s):  
Spotted Fever ◽  
Fluorescent Antibody ◽  
Rocky Mountain ◽  
Fluorescent Antibody Technique ◽  
Rocky Mountain Spotted Fever ◽  
Antibody Technique

Antibody response to Rocky Mountain spotted fever

1976 ◽  
Vol 3 (5) ◽  
pp. 513-518
Author(s):  
R H Kenyon ◽  
P G Canonico ◽  
L S Sammons ◽  
L R Bagley ◽  
C E Pedersen
Keyword(s):  
Antibody Response ◽  
Complement Fixation ◽  
Spotted Fever ◽  
Fluorescent Antibody ◽  
Rocky Mountain ◽  
Fluorescent Antibody Technique ◽  
Rocky Mountain Spotted Fever ◽  
Antibody Technique ◽  
Sensitive Method

Various techniques were compared to determine the most sensitive method for detection of rocky Mountain spotted fever antibody. A radiometabolic technique for detection of Rocky Mountain spotted fever antibody is also described. In infected monkeys, the fluorescent antibody technique yielded the earliest evidence of seroconversion; with some monkeys the microagglutination procedure was equally effective. The fluorescent antibody and microagglutination measurements showed higher titers than those for complement fixation, Weil-Felix, or the radiometabolic techniques.

Hemocytic rickettsia-like organisms inIxodes scapularis: transovarial and transstadial transmission

1995 ◽  
Vol 73 (7) ◽  
pp. 1380-1383
Author(s):  
Louis A. Magnarelli ◽  
Thomas N. Mather ◽  
Min-Tsung Yeh
Keyword(s):  
Rhode Island ◽  
Ixodes Scapularis ◽  
Spotted Fever ◽  
Fluorescent Antibody ◽  
Rocky Mountain ◽  
Rocky Mountain Spotted Fever ◽  
Antibody Staining ◽  
Host Seeking ◽  
Staining Methods ◽  
Fluorescent Antibody Staining

Unengorged, host-seeking female Ixodes scapularis were collected from the northeastern United States (Rhode Island and Massachusetts) in April 1992. Hemolymph preparations from F1males and females were tested to determine if hemocytic rickettsia-like organisms, having shared antigens with ehrlichiae, are passed transovarially and transstadially. Fluorescein-labeled dog anti-Ehrlichia canis antiserum was used to detect infected hemocytes by means of direct fluorescent antibody staining methods. In duplicate tests of F1ticks, hemocytes from 25 males and 25 females were tested by indirect fluorescent antibody staining methods with antisera from two persons who had Rocky Mountain spotted fever. These hemolymph preparations were nonreactive, but those from cohorts of ticks reared from five infected females and tested with anti-E. canis antiserum contained rickettsia-like organisms in the cytoplasm of hemocytes. Thirty-four (54.8%) of 62 males and 71 (63.4%) of 112 females were positive. Prevalence of positive males among the five cohorts ranged from 18.8 to 80%, while results for females varied between 21.2 and 91.7%. The unidentified rickettsia-like hemocytic organisms, which have shared antigens with Ehrlichia species, are transmitted transovarially and transstadially in I. scapularis.

Central Nervous System Dysfunction Associated With Rocky Mountain Spotted Fever Infection in Five Dogs

2005 ◽  
Vol 41 (4) ◽  
pp. 259-266 ◽  
Author(s):  
Jessica S. Mikszewski ◽  
Charles H. Vite
Keyword(s):  
Spotted Fever ◽  
Fluorescent Antibody ◽  
Clinical Signs ◽  
Rocky Mountain ◽  
Rocky Mountain Spotted Fever ◽  
Rickettsia Rickettsii ◽  
Neurological Signs ◽  
Central Nervous System Dysfunction ◽  
Enzyme Screening ◽  
Time Of Presentation

Five dogs from the northeastern United States were presented with clinical signs of neurological disease associated with Rocky Mountain spotted fever (RMSF) infection. Four of the five dogs had vestibular system dysfunction. Other neurological signs included paresis, tremors, and changes in mentation. All of the dogs had an elevated indirect fluorescent antibody titer or a positive semiquantitative enzyme screening immunoassay titer for Rickettsia rickettsii at the time of presentation. Although a higher mortality rate has been reported for dogs with neurological symptoms and RMSF infection, all of the dogs in this study improved with appropriate medical therapy and supportive care.

Spotted fever group rickettsiae in immature and adult ticks (Acari: Ixodidae) from a focus of Rocky Mountain spotted fever in Connecticut

1985 ◽  
Vol 31 (12) ◽  
pp. 1131-1135 ◽  
Author(s):  
Louis A. Magnarelli ◽  
John F. Anderson ◽  
Willy Burgdorfer ◽  
Robert N. Philip ◽  
W. Adrian Chappell
Keyword(s):  
Spotted Fever ◽  
Fluorescent Antibody ◽  
Rocky Mountain ◽  
Dermacentor Variabilis ◽  
Etiologic Agent ◽  
Ixodid Ticks ◽  
Spotted Fever Group ◽  
Rocky Mountain Spotted Fever ◽  
Serum Samples ◽  
Rickettsia Rickettsii

Immature and adult ixodid ticks were collected during 1983 and 1984 in Newtown, Connecticut, an area endemic for Rocky Mountain spotted fever (RMSF), to determine prevalence of infection by spotted fever group (SFG) rickettsiae. Direct fluorescent-antibody (FA) staining revealed SFG organisms in 6 (1.8%) of 332 Dermacentor variabilis larvae, 5 (7.8%) of 64 D. variabilis nymphs, and in 2 (40%) of 5 Ixodes cookei nymphs removed from small- and medium-sized mammals. Hemolymph tests detected rickettsia-like organisms in 15 (8.8%) of 170 D. variabilis adults; 8 specimens retested by direct FA were negative. In contrast, hemocytes from 5 (8.6%) of 58 Ixodes texanus females contained organisms that stained positively in both hemolymph and direct FA tests. An indirect microimmunofluorescence test identified specific antibodies to Rickettsia rickettsii, the etiologic agent of RMSF, in serum samples from a chipmunk, raccoons, and white-footed mice. Results indicate that immature or adult ticks of at least three species may be involved in the maintenance and transmission of SFG rickettsiae at Newtown.

Mitbringsel aus den Ferien

Praxis ◽  
2005 ◽  
Vol 94 (47) ◽  
pp. 1869-1870
Author(s):  
Balestra ◽  
Nüesch
Keyword(s):  
Rocky Mountains ◽  
Spotted Fever ◽  
Rocky Mountain ◽  
Rickettsia Conorii ◽  
Rocky Mountain Spotted Fever ◽  
Klinische Diagnose ◽  
Rickettsia Rickettsii

Eine 37-jährige Patientin stellt sich nach der Rückkehr von einer Rundreise durch Nordamerika mit einem Status febrilis seit zehn Tagen und einem makulösem extremitätenbetontem Exanthem seit einem Tag vor. Bei suggestiver Klinik und Besuch der Rocky Mountains wird ein Rocky Mountain spotted fever diagnostiziert. Die Serologie für Rickettsia conorii, die mit Rickettsia rickettsii kreuzreagiert, war positiv und bestätigte die klinische Diagnose. Allerdings konnte der beweisende vierfache Titeranstieg, möglicherweise wegen spät abgenommener ersten Serologie, nicht nachgewiesen werden. Nach zweiwöchiger antibiotischer Therapie mit Doxycycline waren Status febrilis und Exanthem regredient.

Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

2000 ◽  
Vol 41 (4-5) ◽  
pp. 301-308 ◽  
Author(s):  
N. Noda ◽  
H. Ikuta ◽  
Y. Ebie ◽  
A. Hirata ◽  
S. Tsuneda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Antibody Method ◽  
In Situ Detection ◽  
Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

Mosquito and Tick-borne Illnesses in the United States. Guidelines for the Recognition and Empiric Treatment of Zoonotic Diseases in the Wilderness.

2019 ◽  
Vol 19 (3) ◽  
pp. 238-257
Author(s):  
Suresh Antony
Keyword(s):  
United States ◽  
Lyme Disease ◽  
Spotted Fever ◽  
Disease Process ◽  
Rocky Mountain ◽  
The United States ◽  
Health Care Facilities ◽  
Diagnostic Tools ◽  
Rocky Mountain Spotted Fever ◽  
Number Of Patients

Background:In the United States, tick-borne illnesses account for a significant number of patients that have been seen and treated by health care facilities. This in turn, has resulted in a significant morbidity and mortality and economic costs to the country.Methods:The distribution of these illnesses is geographically variable and is related to the climate as well. Many of these illnesses can be diagnosed and treated successfully, if recognized and started on appropriate antimicrobial therapy early in the disease process. Patient with illnesses such as Lyme disease, Wet Nile illness can result in chronic debilitating diseases if not recognized early and treated.Conclusion:This paper covers illnesses such as Lyme disease, West Nile illness, Rocky Mountain Spotted fever, Ehrlichia, Tularemia, typhus, mosquito borne illnesses such as enteroviruses, arboviruses as well as arthropod and rodent borne virus infections as well. It covers the epidemiology, clinical features and diagnostic tools needed to make the diagnosis and treat these patients as well.

Rubella Antibodies in Human Serum: Detection by the Indirect Fluorescent-Antibody Technique

Science ◽  
1964 ◽  
Vol 145 (3635) ◽  
pp. 943-945 ◽  
Author(s):  
G. C. Brown ◽  
H. F. Maassab ◽  
J. A. Veronelli ◽  
T. J. Francis
Keyword(s):  
Human Serum ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Serum Detection
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