Effect of Inhibitors of Neuronal and Extraneuronal Uptake on the Accumulation and Metabolism of 3H-l-Norepinephrine in Rabbit Aorta

1983 ◽  
Vol 20 (5) ◽  
pp. 234-244 ◽  
Author(s):  
Jerome A. Levin ◽  
Susan E. Wilson
Keyword(s):  
Rabbit Aorta ◽  
Extraneuronal Uptake ◽  
Effect Of Inhibitors

Ketamine: evidence of tissue specific inhibition of neuronal and extraneuronal catecholamine uptake processes

1985 ◽  
Vol 63 (4) ◽  
pp. 298-303 ◽  
Author(s):  
Paul M. Lundy ◽  
Sharunas Gverzdys ◽  
Robert Frew
Keyword(s):  
Mechanism Of Action ◽  
Rabbit Aorta ◽  
Adrenergic Innervation ◽  
Extraneuronal Uptake ◽  
Specific Inhibition ◽  
Chemical Sympathectomy ◽  
Rat Anococcygeus Muscle ◽  
Extraneuronal Catecholamine Uptake ◽  
6 Hydroxydopamine

Ketamine (1.1 × 10−5 to 3.7 × 10−4 M) potentiated catecholamine responses of rat anococcygeus muscle and rabbit aorta in vitro. In the anococcygeus, potentiation was abolished by cocaine (2.9 × 10−5 M) pretreatment or by chemical sympathectomy using 6-hydroxydopamine (6-OHDA), but was unaffected by pretreatment with the extraneuronal uptake inhibitor cortisol (8.3 × 10−5 M), or the catechol-O-methyltransferase inhibitor tropolone (2.4 × 10−4 M). The action of ketamine mimicked the potentiating effect of cocaine on tyramine responses. In contrast, the potentiation by ketamine in rabbit aorta was unaffected by cocaine or 6-OHDA but was abolished by cortisol or tropolone; and ketamine potentiated tyramine responses, whereas cocaine inhibited them. Thus the mechanism of action by which ketamine produces potentiation of catecholamines in these two tissues is completely different. These results suggest that ketamine has the unusual ability to block neuronal and extraneuronal uptake and that the predominating mechanism will depend on the type of tissue examined and the morphology of its adrenergic innervation.

Extraneuronal uptake and O-methylation of 3H-adrenaline in the rabbit aorta

1993 ◽  
Vol 347 (4) ◽  
pp. 363-370 ◽  
Author(s):  
F�tima Martel ◽  
Isabel Azevedo ◽  
Walter Osswald
Keyword(s):  
Rabbit Aorta ◽  
Extraneuronal Uptake

Ketamine potentiates catecholamine responses of vascular smooth muscle by inhibition of extraneuronal uptake

1981 ◽  
Vol 59 (6) ◽  
pp. 520-527 ◽  
Author(s):  
Paul M. Lundy ◽  
Robert Frew
Keyword(s):  
Smooth Muscles ◽  
Rabbit Aorta ◽  
Extraneuronal Uptake ◽  
Neuronal Uptake ◽  
Vascular Smooth Muscles ◽  
Comt Inhibitors ◽  
Relaxant Effect ◽  
17Β Estradiol ◽  
Uptake Studies ◽  
6 Hydroxydopamine

Effects of ketamine on responses to sympathomimetic amines were studied using isolated aortic and pulmonary artery strips from the rabbit. Ketamine (1.1 × 10−5 to 3.7 × 10−4 M) potentiated adrenaline-contracted strips. Potentiation was not impaired in tissues from animals pretreated with reserpine, with 6-hydroxydopamine, or in tissues pretreated with cocaine. Pretreatment of the strips with the catechol O-methyltransferase (COMT) inhibitors tropolone or pyrogallol or the inhibitor of extraneuronal uptake 17β-estradiol blocked the potentiation by ketamine; in addition, potentiation by the COMT and extraneuronal uptake inhibitors was abolished or greatly reduced by ketamine. In rabbit aorta, ketamine potentiated responses to the catecholamines (adrenaline > nordefrine > noradrenaline) but not to the noneatecholamines phenylephrine, methoxamine, and synephrine; instead a slight relaxant effect was observed. Ketamine potentiated, whereas cocaine inhibited, responses to tyramine. Experiments using the technique of oil immersion demonstrated that ketamine reduced the rate at which aortic strips inactivate adrenaline even when monoamine oxidase (MAO) and neuronal uptake processes were fully inhibited. Uptake studies showed that ketamine and 17β-estradiol reduced extraneuronal accumulation of [3H]adrenaline in aortic strips. We conclude that ketamine is an inhibitor of extraneuronal uptake in the vascular smooth muscles studied and the importance of this mechanism in producing its known cardiovascular effect is discussed.

The Cellular Origin in Atheromatous Lesion

1970 ◽  
Vol 28 ◽  
pp. 202-203
Author(s):  
T. M. Murad ◽  
H. A. I. Newman ◽  
K. F. Kern
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Rabbit Aorta ◽  
Mixed Population ◽  
The Other ◽  
Cellular Origin ◽  
Ultrastructural Studies ◽  
Atherosclerotic Lesions ◽  
Show Evidence ◽  
Early Lesion

The origin of lipid containing cells in atheromatous lesion has been disputed. Geer in his study on atheromatous lesions of rabbit aorta, suggested that the early lesion is composed mainly of lipid-laden macrophages and the later lesion has a mixed population of macrophages and smooth muscle cells. Parker on the other hand, was able to show evidence that the rabbit lesion is primarily composed of lipid-laden cells of smooth muscle origin. The above studies and many others were done on an intact lesion without any attempt of cellular isolation previous to their ultrastructural studies. Cell isolation procedures have been established for atherosclerotic lesions through collagenase and elastase digestion Therefore this procedure can be utilized to identify the cells involved in rabbit atheroma.

Neuronal and Extraneuronal Uptake of 3H-Norepinephrine in the Heart of the Active Bat: An Electron Microscopic Radioautographic Study

1973 ◽  
Vol 31 ◽  
pp. 522-523
Author(s):  
Martin Hagopian ◽  
Michael D. Gershon ◽  
Eladio A. Nunez
Keyword(s):  
Smooth Muscle ◽  
Monoamine Oxidase ◽  
Vascular Smooth Muscle ◽  
Cardiac Muscle ◽  
Maximum Rate ◽  
External Medium ◽  
Extraneuronal Uptake ◽  
Electron Microscopic ◽  
Cardiac Tissues ◽  
High Affinity

The ability of cardiac tissues to take up norepinephrine from an external medium is well known. Two mechanisms, called Uptake and Uptake respectively by Iversen have been differentiated. Uptake is a high affinity system associated with adrenergic neuronal elements. Uptake is a low affinity system, with a higher maximum rate than that of Uptake. Uptake has been associated with extraneuronal tissues such as cardiac muscle, fibroblasts or vascular smooth muscle. At low perfusion concentrations of norepinephrine most of the amine taken up by Uptake is metabolized. In order to study the localization of sites of norepinephrine storage following its uptake in the active bat heart, tritiated norepinephrine (2.5 mCi; 0.064 mg) was given intravenously to 2 bats. Monoamine oxidase had been inhibited with pheniprazine (10 mg/kg) one hour previously to decrease metabolism of norepinephrine.

Sign in / Sign up

Export Citation Format

Share Document