Red Cell NADH Diaphorase Variants in Japanese

1985 ◽  
Vol 35 (4) ◽  
pp. 212-217 ◽  
Author(s):  
K. Shimizu ◽  
H. Keino ◽  
A. Mizutani ◽  
T. Itoh ◽  
I. Nishigaki
Keyword(s):  
Red Cell ◽  
Nadh Diaphorase

scholarly journals A Simple Spot Screening Test for Fast Detection of Red Cell NADH-Diaphorase Deficiency

Blood ◽  
1970 ◽  
Vol 36 (3) ◽  
pp. 330-333 ◽  
Author(s):  
JEAN-CLAUDE KAPLAN ◽  
ANNE-MARIE NICOLAS ◽  
ALENA HANZLICKOVA-LEROUX ◽  
ERNEST BEUTLER
Keyword(s):  
Filter Paper ◽  
Screening Test ◽  
Red Cell ◽  
Fast Detection ◽  
Nadh Diaphorase

Abstract A rapid spot screening test for NADH-diaphorase deficiency is described. Nitrited blood is added to a reaction mixture freshly prepared from stable stock reagents. Spots are made at intervals on filter paper and examined for defluorescence as NADH is oxidized. All of six patients with NADH diaphorase deficiency studied have been correctly identified using this technique, but it is not suitable for the detection of heterozygotes.

Red cell NADH-diaphorase polymorphism in Macaca mulatta

1977 ◽  
Vol 6 (3) ◽  
pp. 227-230 ◽  
Author(s):  
W LOS ◽  
P MEERAKHAN
Keyword(s):  
Macaca Mulatta ◽  
Red Cell ◽  
Nadh Diaphorase

Study of Human Red Cell NADH Diaphorase (Dia1) in the Italian Population

1985 ◽  
Vol 35 (1) ◽  
pp. 7-10 ◽  
Author(s):  
M. Beretta ◽  
A.S. Santachiara-Benerecetti
Keyword(s):  
Italian Population ◽  
Red Cell ◽  
Nadh Diaphorase

Genetically determined electrophoretic variants of human red cell NADH diaphorase

1970 ◽  
Vol 34 (1) ◽  
pp. 1-10 ◽  
Author(s):  
D. A. HOPKINSON ◽  
G. CORNEY ◽  
P. J. L. COOK ◽  
E. B. ROBSON ◽  
HARRY HARRIS
Keyword(s):  
Red Cell ◽  
Electrophoretic Variants ◽  
Nadh Diaphorase ◽  
Genetically Determined

scholarly journals Congenital Methemoglobinemia: The Result of Age-dependent Decay of Methemoglobin Reductase

Blood ◽  
1972 ◽  
Vol 39 (3) ◽  
pp. 407-414 ◽  
Author(s):  
Stephen A. Feig ◽  
David G. Nathan ◽  
Park S. Gerald ◽  
Harold S. Zarkowski
Keyword(s):  
Specific Activity ◽  
Reductase Activity ◽  
Red Cells ◽  
Enzyme Electrophoresis ◽  
Red Cell ◽  
Molecular Abnormalities ◽  
Diaphorase Activity ◽  
Nadh Diaphorase ◽  
Age Dependent ◽  
Substrate Affinities

Abstract Methemoglobin reductase activity (MHR), measured by the Hegesh assay, decreases as normal red cells age. This probably accounts for the slighlty increased concentration of methemoglobin in older red cells. Exaggeration of normal MHR age lability was demonstrated in several individuals with various molecular abnormalities of NADH-diaphorase and was associated with more striking accumulation of methemoglobin in the older red cells of those patients. Diaphorase activity, measured by the Scott assay, does not exhibit age lability in normal red cells or the cells of affected patients. While diaphorase uses either NADH or NADPH as a cofactor, methemoglobin reductase requires NADH. Methemoglobin reductase is found in hemolysates but not in membrane preparations, while approximately 1% of total red cell NADH-diaphorase activity is membrane-associated. The specific activity of NADH-diaphorase is approximately 60 times greater than that of methemoglobin reductase. Whether the two activities represent distinct enzyme proteins or differing substrate affinities of the same enzyme awaits adaptation of the Hegesh assay to enzyme electrophoresis.

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