scholarly journals SOME OBSERVATIONS ON THE INCIDENCE OF DRUMSTICKS IN POLYMORPHONUCLEAR NEUTROPHIL LEUCOCYTES OF FEMALES

1956 ◽  
Vol 6 (2) ◽  
pp. 263-263
Author(s):  
U. Mittwoch
Keyword(s):  
Polymorphonuclear Neutrophil

Interaction of Neutrophils with Endothelial Cells, Fibroblasts and Their Extracellular Matrices: Microscopic and Computerised Analysis

1988 ◽  
Vol 16 (1) ◽  
pp. 48-53
Author(s):  
Marina Ziche ◽  
Lucia Morbidelli ◽  
Annalisa Rubino ◽  
Piero Dolara ◽  
Stefano Bianchi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Image Analysis ◽  
Vascular Endothelial Cells ◽  
Extracellular Matrices ◽  
Polymorphonuclear Neutrophil ◽  
Initial Event ◽  
Computerised Image Analysis ◽  
Capillary Endothelial Cells ◽  
Vitro Model

Polymorphonuclear neutrophil (PMN) interaction with vascular endothelial cells is the initial event in the migration of neutrophils through blood vessel walls before reaching inflammation sites in tissues. The interaction between fibroblasts and endothelial cells and their extracellular matrices might be modulated by the activation of neutrophils that occurs at inflammatory reaction sites. We have used an in vitro model to study PMN function, measuring the adhesion of human PMNs to capillary endothelial cells and fibroblasts grown in culture and to their extracellular matrices. The interaction was measured in basal conditions and in the presence of the chemotactic effector, formyl-methionyl-leucyl-phenylalanine (FMLP at the concentration of 10 7M). Adhesion was expressed by the number of adherent PMNs/mm2 on a histological specimen. Moreover, we have adapted a program for image analysis to quantify neutrophil adhesion. Three times more PMNs adhered to matrices than to monolayers, and adherence could be increased by the presence of 10-7M FMLP, except in the case of fibroblast monolayers. We found a good correlation between microscopic observation and computerised image analysis measuring PMN adhesiveness to extracellular matrices.

Acquired abnormalities of polymorphonuclear neutrophil function

Blood Reviews ◽  
1990 ◽  
Vol 4 (2) ◽  
pp. 103-110 ◽  
Author(s):  
J. Fletcher ◽  
A.P. Haynes ◽  
S.M. Crouch
Keyword(s):  
Neutrophil Function ◽  
Polymorphonuclear Neutrophil ◽  
Acquired Abnormalities

scholarly journals Characterization of the Priming Effect by Pituitary Canine Growth Hormone on Canine Polymorphonuclear Neutrophil Granulocyte Function

2000 ◽  
Vol 7 (2) ◽  
pp. 226-232 ◽  
Author(s):  
Thomas K. Petersen ◽  
C. Wayne Smith ◽  
Asger L. Jensen
Keyword(s):  
Growth Hormone ◽  
Priming Effect ◽  
Shape Change ◽  
Transendothelial Migration ◽  
Lower Surface ◽  
Polymorphonuclear Neutrophil ◽  
Neutrophil Granulocytes ◽  
Neutrophil Granulocyte ◽  
Microtiter Plates ◽  
Granulocyte Function

ABSTRACT In this report, we demonstrate that canine growth hormone (cGH) is capable of priming canine polymorphonuclear neutrophil granulocytes (PMN) in a manner resembling that of human PMN. The cGH influences important functions that are involved in the process of recruitment of PMN, i.e., shape change, chemotaxis, CD11b/CD18 expression, adhesion, and subsequent transendothelial migration. Also, intracellular O2 − production was evaluated. We investigated the priming effect by incubating PMN with purified pituitary cGH at various concentrations (10 to 800 μg/liter). The capacity for shape change was significantly (P < 0.05) enhanced, whereas the chemotactic response under agarose was significantly (P < 0.05) reduced. The chemotactic migration in Boyden chambers (10-μm-thick polycarbonate filter; lower surface count technique) was significantly (P < 0.05) enhanced, presumably due to cGH-induced hyperadhesiveness to the lower surface of the filters. The adhesion in albumin-coated microtiter plates and adherence to canine pulmonary fibroblasts were significantly (P < 0.05) increased, and the increased adhesion resulted in a significant (P < 0.01) increase in transendothelial migration using canine jugular vein endothelial cells. The increase in adhesion was associated with a significant increase in CD11b/CD18 expression. Furthermore, intracellular O2 − production was significantly enhanced in response to both phorbol myristate acetate (P < 0.01) and opsonized zymosan (P < 0.05). In the absence of a PMN-stimulating agent, cGH did not influence the effector functions investigated except for an increased expression of CD11b/CD18.

EFFECT OF SKELETAL MUSCLE CONTUSION ON POLYMORPHONUCLEAR NEUTROPHIL (PMN) ROLLING

1996 ◽  
Vol 89 (Supplement) ◽  
pp. S90
Author(s):  
Al Marr ◽  
Thomas Smith ◽  
Walton Curl ◽  
Eileen Rosencrance
Keyword(s):  
Skeletal Muscle ◽  
Polymorphonuclear Neutrophil ◽  
Skeletal Muscle Contusion

scholarly journals PR-39, a potent neutrophil inhibitor, attenuates myocardial ischemia-reperfusion injury in mice

2000 ◽  
Vol 279 (6) ◽  
pp. H2824-H2828 ◽  
Author(s):  
Michaela R. Hoffmeyer ◽  
Rosario Scalia ◽  
Chris R. Ross ◽  
Steven P. Jones ◽  
David J. Lefer
Keyword(s):  
Myocardial Ischemia ◽  
Reperfusion Injury ◽  
Murine Model ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Polymorphonuclear Neutrophil ◽  
Leukocyte Rolling ◽  
Rat Mesentery ◽  
Myocardial Ischemia Reperfusion Injury ◽  
Myocardial Ischemia Reperfusion

We investigated the effects of PR-39, a recently discovered neutrophil inhibitor, in a murine model of myocardial ischemia-reperfusion injury. Mice were given an intravenous injection of vehicle ( n = 12) or PR-39 ( n = 9) and subjected to 30 min of coronary artery occlusion followed by 24 h of reperfusion. In addition, the effects of PR-39 on leukocyte rolling and adhesion were studied utilizing intravital microscopy of the rat mesentery. The area-at-risk per left ventricle was similar in vehicle- and PR-39-treated mice. However, myocardial infarct per risk area was significantly ( P < 0.01) reduced in PR-39 treated hearts (21.0 ± 3.8%) compared with vehicle (47.1 ± 4.8%). Histological analysis of ischemic reperfused myocardium demonstrated a significant ( P < 0.01) reduction in polymorphonuclear neutrophil (PMN) accumulation in PR-39-treated hearts ( n = 6, 34.3 ± 1.7 PMN/mm2) compared with vehicle-treated myocardium ( n = 6, 59.7 ± 3.1 PMN/mm2). In addition, PR-39 significantly ( P < 0.05) attenuated leukocyte rolling and adherence in rat inflamed mesentery. These results indicate that PR-39 inhibits leukocyte recruitment into inflamed tissue and attenuated myocardial reperfusion injury in a murine model of myocardial ischemia-reperfusion.

scholarly journals An ultra-sensitive, ultra-fast whole blood monocyte CD169 assay for COVID-19 screening

2020 ◽  
Author(s):  
Moïse Michel ◽  
Fabrice Malergue ◽  
Inès Ait Belkacem ◽  
Pénélope Bourgoin ◽  
Pierre-Emmanuel Morange ◽  
...  
Keyword(s):  
Whole Blood ◽  
Bacterial Infections ◽  
Viral Infections ◽  
Early Stage ◽  
Polymorphonuclear Neutrophil ◽  
Blood Monocyte ◽  
Rt Pcr ◽  
Neutrophil Cd64 ◽  
Asymptomatic Patients ◽  
Hla Dr

AbstractCoVID-19 is an unprecedented epidemic, globally challenging health systems, societies, and economy. Its diagnosis relies on molecular methods, with drawbacks revealed by current use as mass screening. Monocyte CD169 upregulation has been reported as a marker of viral infections, we evaluated a flow cytometry three-color rapid assay of whole blood monocyte CD169 for CoVID-19 screening.Outpatients (n=177) with confirmed CoVID-19 infection, comprising 80 early-stage (≤14 days after symptom onset), 71 late-stage (≥15 days), and 26 asymptomatic patients received whole blood CD169 testing in parallel with SARS-CoV-2 RT-PCR. Upregulation of monocyte CD169 without polymorphonuclear neutrophil CD64 changes was the primary endpoint. Sensitivity was 98% and 100% in early-stage and asymptomatic patients respectively, specificity was 50% and 84%. Rapid whole blood monocyte CD169 evaluation was highly sensitive when compared with RT-PCR, especially in early-stage, asymptomatic patients whose RT-PCR tests were not yet positive.Diagnostic accuracy, easy finger prick sampling and minimal time-to-result (15-30 minutes) rank whole blood monocyte CD169 upregulation as a potential screening and diagnostic support for CoVID-19. Secondary endpoints were neutrophil CD64 upregulation as a marker of bacterial infections and monocyte HLA-DR downregulation as a surrogate of immune fitness, both assisting with adequate and rapid management of non-CoVID cases.

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