Differentiation of Biologically Distinct Cucumber Mosaic Virus Isolates by PAGE of Double-Stranded RNA

Intervirology ◽  
1992 ◽  
Vol 34 (1) ◽  
pp. 23-29 ◽  
Author(s):  
R.D. Pares ◽  
M.R. Gillings ◽  
L.V. Gunn
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Double Stranded Rna ◽  
Virus Isolates

scholarly journals Comparison of cucumber mosaic virus isolates by double-stranded RNA analysis.

1988 ◽  
Vol 54 (4) ◽  
pp. 536-539 ◽  
Author(s):  
Wei Qin WANG ◽  
Tomohide NATSUAKI ◽  
Seiichi OKUDA ◽  
Michiaki TERANAKA
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Double Stranded Rna ◽  
Rna Analysis ◽  
Virus Isolates

scholarly journals Topical Application of Double-Stranded RNA Targeting 2b and CP Genes of Cucumber mosaic virus Protects Plants against Local and Systemic Viral Infection

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 963
Author(s):  
Maria C. Holeva ◽  
Athanasios Sklavounos ◽  
Rajendran Rajeswaran ◽  
Mikhail M. Pooggin ◽  
Andreas E. Voloudakis
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Topical Application ◽  
Plant Virus ◽  
Plant Protection ◽  
Post Inoculation ◽  
Tobacco Plants ◽  
Double Stranded Rna

Cucumber mosaic virus (CMV) is a destructive plant virus with worldwide distribution and the broadest host range of any known plant virus, as well as a model plant virus for understanding plant–virus interactions. Since the discovery of RNA interference (RNAi) as a major antiviral defense, RNAi-based technologies have been developed for plant protection against viral diseases. In plants and animals, a key trigger of RNAi is double-stranded RNA (dsRNA) processed by Dicer and Dicer-like (DCL) family proteins in small interfering RNAs (siRNAs). In the present study, dsRNAs for coat protein (CP) and 2b genes of CMV were produced in vitro and in vivo and applied onto tobacco plants representing a systemic solanaceous host as well as on a local host plant Chenopodium quinoa. Both dsRNA treatments protected plants from local and systemic infection with CMV, but not against infection with unrelated viruses, confirming sequence specificity of antiviral RNAi. Antiviral RNAi was effective when dsRNAs were applied simultaneously with or four days prior to CMV inoculation, but not four days post inoculation. In vivo-produced dsRNAs were more effective than the in vitro-produced; in treatments with in vivo dsRNAs, dsRNA-CP was more effective than dsRNA-2b, while the effects were opposite with in vitro dsRNAs. Illumina sequencing of small RNAs from in vivo dsRNA-CP treated and non-treated tobacco plants revealed that interference with CMV infection in systemic leaves coincides with strongly reduced accumulation of virus-derived 21- and 22-nucleotide (nt) siRNAs, likely generated by tobacco DCL4 and DCL2, respectively. While the 21-nt class of viral siRNAs was predominant in non-treated plants, 21-nt and 22-nt classes accumulated at almost equal (but low) levels in dsRNA treated plants, suggesting that dsRNA treatment may boost DCL2 activity. Taken together, our findings confirm the efficacy of topical application of dsRNA for plant protection against viruses and shed more light on the mechanism of antiviral RNAi.

scholarly journals Genetic Structure and Molecular Variability of Cucumber mosaic virus Isolates in the United States

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e96582 ◽  
Author(s):  
Shahideh Nouri ◽  
Rafael Arevalo ◽  
Bryce W. Falk ◽  
Russell L. Groves
Keyword(s):  
United States ◽  
Genetic Structure ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
The United States ◽  
Molecular Variability ◽  
Virus Isolates

Comparative transmission of two cucumber mosaic virus isolates by two color morphs of Acyrthosiphon pisum (Harris)

2012 ◽  
Vol 56 (02) ◽  
pp. 139-143 ◽  
Author(s):  
A. Tahmasebi ◽  
A. Dizadji ◽  
F. Farhoudi ◽  
H. Allahyari ◽  
M. Koohi-Habibi
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Acyrthosiphon Pisum ◽  
Color Morphs ◽  
Virus Isolates

scholarly journals Emergence and Characterization of Satellite RNAs Associated with Spanish Cucumber Mosaic Virus Isolates

1984 ◽  
Vol 65 (3) ◽  
pp. 539-547 ◽  
Author(s):  
I. Garcia-Luque ◽  
J. M. Kaper ◽  
J. R. Diaz-Ruiz ◽  
M. Rubio-Huertos
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Satellite Rnas ◽  
Virus Isolates

Differentiation of Cucumber mosaic virus isolates by hybridization to oligonucleotides in a microarray format

2005 ◽  
Vol 123 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Zhang Deyong ◽  
Peter Willingmann ◽  
Cornelia Heinze ◽  
Günter Adam ◽  
Monica Pfunder ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Isolates ◽  
Microarray Format
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