Viral Strain Dependent Differences in Experimental Argentine Hemorrhagic Fever (Junin Virus) Infection of Guinea Pigs

Intervirology ◽  
1988 ◽  
Vol 29 (3) ◽  
pp. 133-143 ◽  
Author(s):  
Richard H. Kenyon ◽  
D.E. Green ◽  
J.I. Maiztegui ◽  
C.J. Peters
Keyword(s):  
Virus Infection ◽  
Guinea Pigs ◽  
Hemorrhagic Fever ◽  
Viral Strain ◽  
Junin Virus ◽  
Argentine Hemorrhagic Fever ◽  
Junín Virus ◽  
Strain Dependent

scholarly journals Favipiravir (T-705) Inhibits Junín Virus Infection and Reduces Mortality in a Guinea Pig Model of Argentine Hemorrhagic Fever

2013 ◽  
Vol 7 (12) ◽  
pp. e2614 ◽  
Author(s):  
Brian B. Gowen ◽  
Terry L. Juelich ◽  
Eric J. Sefing ◽  
Trevor Brasel ◽  
Jennifer K. Smith ◽  
...  
Keyword(s):  
Virus Infection ◽  
Guinea Pig ◽  
Hemorrhagic Fever ◽  
Pig Model ◽  
Junin Virus ◽  
Argentine Hemorrhagic Fever ◽  
Guinea Pig Model ◽  
Junín Virus

Junin virus activity in two rural populations of the Argentine hemorrhagic fever (AHF) endemic area

1983 ◽  
Vol 12 (4) ◽  
pp. 273-280 ◽  
Author(s):  
Mercedes C. Weissenbacher ◽  
Marta S. Sabattini ◽  
María M. Avila ◽  
Patricia M. Sangiorgio ◽  
María R. F. De Sensi ◽  
...  
Keyword(s):  
Endemic Area ◽  
Hemorrhagic Fever ◽  
Rural Populations ◽  
Junin Virus ◽  
Argentine Hemorrhagic Fever ◽  
Virus Activity ◽  
Junín Virus

Effect of Ribavirin on Junin Virus Infection in Guinea Pigs

2012 ◽  
Vol 59 (4) ◽  
pp. 278-285 ◽  
Author(s):  
M. Salazar ◽  
N. E. Yun ◽  
A. L. Poussard ◽  
J. N. Smith ◽  
J. K. Smith ◽  
...  
Keyword(s):  
Virus Infection ◽  
Guinea Pigs ◽  
Junin Virus ◽  
Junín Virus

scholarly journals Novel Arenavirus Entry Inhibitors Discovered by Using a Minigenome Rescue System for High-Throughput Drug Screening

2015 ◽  
Vol 89 (16) ◽  
pp. 8428-8443 ◽  
Author(s):  
Jessica Y. Rathbun ◽  
Magali E. Droniou ◽  
Robert Damoiseaux ◽  
Kevin G. Haworth ◽  
Jill E. Henley ◽  
...  
Keyword(s):  
High Throughput ◽  
Causative Agent ◽  
High Throughput Screening ◽  
Hemorrhagic Fever ◽  
Human Pathogens ◽  
Junin Virus ◽  
Hemorrhagic Fevers ◽  
Argentine Hemorrhagic Fever ◽  
Rescue System ◽  
Junín Virus

ABSTRACTCertain members of theArenaviridaefamily are category A agents capable of causing severe hemorrhagic fevers in humans. Specific antiviral treatments do not exist, and the only commonly used drug, ribavirin, has limited efficacy and can cause severe side effects. The discovery and development of new antivirals are inhibited by the biohazardous nature of the viruses, making them a relatively poorly understood group of human pathogens. We therefore adapted a reverse-genetics minigenome (MG) rescue system based on Junin virus, the causative agent of Argentine hemorrhagic fever, for high-throughput screening (HTS). The MG rescue system recapitulates all stages of the virus life cycle and enables screening of small-molecule libraries under biosafety containment level 2 (BSL2) conditions. The HTS resulted in the identification of four candidate compounds with potent activity against a broad panel of arenaviruses, three of which were completely novel. The target for all 4 compounds was the stage of viral entry, which positions the compounds as potentially important leads for future development.IMPORTANCEThe arenavirus family includes several members that are highly pathogenic, causing acute viral hemorrhagic fevers with high mortality rates. No specific effective treatments exist, and although a vaccine is available for Junin virus, the causative agent of Argentine hemorrhagic fever, it is licensed for use only in areas where Argentine hemorrhagic fever is endemic. For these reasons, it is important to identify specific compounds that could be developed as antivirals against these deadly viruses.

scholarly journals Junín Virus Promotes Autophagy To Facilitate the Virus Life Cycle

2019 ◽  
Vol 93 (15) ◽  
Author(s):  
Julieta S. Roldán ◽  
Nélida A. Candurra ◽  
María I. Colombo ◽  
Laura R. Delgui
Keyword(s):  
Hemorrhagic Fever ◽  
Replication Capacity ◽  
Epidemic Disease ◽  
Junin Virus ◽  
Hemorrhagic Fevers ◽  
Argentine Hemorrhagic Fever ◽  
The Family ◽  
Infected Cells ◽  
Junín Virus

ABSTRACTJunín virus (JUNV), a member of the familyArenaviridae, is the etiological agent of Argentine hemorrhagic fever (AHF), a potentially deadly endemic-epidemic disease affecting the population of the most fertile farming land of Argentina. Autophagy is a degradative process with a crucial antiviral role; however, several viruses subvert the pathway to their benefit. We determined the role of autophagy in JUNV-infected cells by analyzing LC3, a cytoplasmic protein (LC3-I) that becomes vesicle membrane associated (LC3-II) upon induction of autophagy. Cells overexpressing enhanced green fluorescent protein (EGFP)-LC3 and infected with JUNV showed an increased number of LC3 punctate structures, similar to those obtained after starvation or bafilomycin A1 treatment, which leads to autophagosome induction or accumulation, respectively. We also monitored the conversion of LC3-I to LC3-II, observing LC3-II levels in JUNV-infected cells similar to those observed in starved cells. Additionally, we kinetically studied the number of LC3 dots after JUNV infection and found that the virus activated the pathway as early as 2 h postinfection (p.i.), whereas the UV-inactivated virus did not induce the pathway. Cells subjected to starvation or pretreated with rapamycin, a pharmacological autophagy inductor, enhanced virus yield. Also, we assayed the replication capacity of JUNV in Atg5 knockout or Beclin 1 knockdown cells (both critical components of the autophagic pathway) and found a significant decrease in JUNV replication. Taken together, our results constitute the first study indicating that JUNV infection induces an autophagic response, which is functionally required by the virus for efficient propagation.IMPORTANCEMammalian arenaviruses are zoonotic viruses that cause asymptomatic and persistent infections in their rodent hosts but may produce severe and lethal hemorrhagic fevers in humans. Currently, there are neither effective therapeutic options nor effective vaccines for viral hemorrhagic fevers caused by human-pathogenic arenaviruses, except the vaccine Candid no. 1 against Argentine hemorrhagic fever (AHF), licensed for human use in areas of endemicity in Argentina. Since arenaviruses remain a severe threat to global public health, more in-depth knowledge of their replication mechanisms would improve our ability to fight these viruses. Autophagy is a lysosomal degradative pathway involved in maintaining cellular homeostasis, representing powerful anti-infective machinery. We show, for the first time for a member of the familyArenaviridae, a proviral role of autophagy in JUNV infection, providing new knowledge in the field of host-virus interaction. Therefore, modulation of virus-induced autophagy could be used as a strategy to block arenavirus infections.

ChemInform Abstract: Imidazo[2,1-b]thiazole Carbohydrate Derivatives: Synthesis and Antiviral Activity Against Junin Virus, Agent of Argentine Hemorrhagic Fever.

ChemInform ◽  
2011 ◽  
Vol 42 (22) ◽  
pp. no-no
Author(s):  
Jose Sebastian Barradas ◽  
Maria Ines Errea ◽  
Norma B. D'Accorso ◽  
Claudia Soledad Sepulveda ◽  
Elsa Beatriz Damonte
Keyword(s):  
Antiviral Activity ◽  
Hemorrhagic Fever ◽  
Junin Virus ◽  
Argentine Hemorrhagic Fever ◽  
Junín Virus

Prevalence of Infection with Junin Virus in Rodent Populations in the Epidemic Area of Argentine Hemorrhagic Fever

1994 ◽  
Vol 51 (5) ◽  
pp. 554-562 ◽  
Author(s):  
James N. Mills ◽  
C. J. Peters ◽  
James E. Childs ◽  
Kelly T. McKee ◽  
Thomas G. Ksiazek ◽  
...  
Keyword(s):  
Hemorrhagic Fever ◽  
Junin Virus ◽  
Argentine Hemorrhagic Fever ◽  
Epidemic Area ◽  
Rodent Populations ◽  
Junín Virus
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