The Relationship between a 27-nm Virus-Like Particle and Hepatitis A as Demonstrated by Immune Electron Microscopy

Intervirology ◽  
1974 ◽  
Vol 4 (2) ◽  
pp. 110-118 ◽  
Author(s):  
Stephen A. Locarnini ◽  
Allan A. Ferris ◽  
Anthony C. Stott ◽  
Ian D Gust
Keyword(s):  
Electron Microscopy ◽  
Hepatitis A ◽  
Immune Electron Microscopy ◽  
Virus Like Particle ◽  
The Relationship

Immune Electron Microscopy (IEM) of a Canine Adeno-Associated Virus

1974 ◽  
Vol 32 ◽  
pp. 256-257
Author(s):  
Gunter F. Thomas ◽  
M. David Hoggan
Keyword(s):  
Electron Microscopy ◽  
Cell Cultures ◽  
Complement Fixation ◽  
Hepatitis Virus ◽  
Wide Distribution ◽  
Immune Electron Microscopy ◽  
Adeno Associated Virus ◽  
Virus Like Particle ◽  
Dog Kidney ◽  
Green Monkeys

In 1968, Sugimura and Yanagawa described a small 25 nm virus like particle in association with the Matsuda strain of infectious canine hepatitis virus (ICHV). Domoto and Yanagawa showed that this particle was dependent on ICHV for its replication in primary dog kidney cell cultures (PDK) and was resistant to heating at 70°C for 10 min, and concluded that it was a canine adeno-associated virus (CAAV). Later studies by Onuma and Yanagawa compared CAAV with the known human serotypes (AAV 1, 2, 3) and AAV-4, known to be associated with African Green Monkeys. Using the complement fixation (CF) test, they found that CAAV was serologically related to AAV-3 and had wide distribution in the dog population of Japan.

Application of solid-phase immune electron microscopy to study physical and stability characteristics of enterically transmitted non-a, non-b hepatitis virus

1988 ◽  
Vol 46 ◽  
pp. 80-81
Author(s):  
Charles D. Humphrey ◽  
E. H. Cook ◽  
Karen A. McCaustland ◽  
Daniel W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Etiologic Agent ◽  
World Health ◽  
Health Concern ◽  
Morphological Identification ◽  
Immune Electron Microscopy

Enterically transmitted non-A, non-B hepatitis (ET-NANBH) is a type of hepatitis which is increasingly becoming a significant world health concern. As with hepatitis A virus (HAV), spread is by the fecal-oral mode of transmission. Until recently, the etiologic agent had not been isolated and identified. We have succeeded in the isolation and preliminary characterization of this virus and demonstrating that this agent can cause hepatic disease and seroconversion in experimental primates. Our characterization of this virus was facilitated by immune (IEM) and solid phase immune electron microscopic (SPIEM) methodologies.Many immune electron microscopy methodologies have been used for morphological identification and characterization of viruses. We have previously reported a highly effective solid phase immune electron microscopy procedure which facilitated identification of hepatitis A virus (HAV) in crude cell culture extracts. More recently we have reported utilization of the method for identification of an etiologic agent responsible for (ET-NANBH).

Identification of hepatitis a virus in cell cultures by solid phase immune electron microscopy

1986 ◽  
Vol 44 ◽  
pp. 238-239
Author(s):  
C.D. Humphrey ◽  
T.L. Cromeans ◽  
E.H. Cook ◽  
D.W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Liquid Phase ◽  
Cell Cultures ◽  
Rapid Detection ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Immune Electron Microscopy ◽  
Detection And Identification

There is a variety of methods available for the rapid detection and identification of viruses by electron microscopy as described in several reviews. The predominant techniques are classified as direct electron microscopy (DEM), immune electron microscopy (IEM), liquid phase immune electron microscopy (LPIEM) and solid phase immune electron microscopy (SPIEM). Each technique has inherent strengths and weaknesses. However, in recent years, the most progress for identifying viruses has been realized by the utilization of SPIEM.

scholarly journals Quantitation of antibody to hepatitis A antigen by immune electron microscopy.

1976 ◽  
Vol 13 (4) ◽  
pp. 1209-1213 ◽  
Author(s):  
J L Dienstag ◽  
D W Alling ◽  
R H Purcell
Keyword(s):  
Electron Microscopy ◽  
Hepatitis A ◽  
Immune Electron Microscopy

scholarly journals Enzyme-Linked Immunosorbent Assay for Detection of Hepatitis A Antigen in Stool and Antibody to Hepatitis A Antigen in Sera: Comparison with Solid-Phase Radioimmunoassay, Immune Electron Microscopy, and Immune Adherence Hemagglutination Assay

1978 ◽  
Vol 7 (2) ◽  
pp. 184-193
Author(s):  
Lars R. Mathiesen ◽  
Stephen M. Feinstone ◽  
Doris C. Wong ◽  
Peter Skinhoej ◽  
Robert H. Purcell
Keyword(s):  
Electron Microscopy ◽  
Hepatitis A ◽  
Solid Phase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immune Electron Microscopy ◽  
Specific Detection ◽  
Immune Adherence ◽  
Ag Particles ◽  
Solid Phase Radioimmunoassay ◽  
Human Stool

Previously described techniques for detection of hepatitis A antigen (HA Ag) and antibody (anti-HA) have required purified HA Ag and expensive equipment. Herein is described an enzyme-linked immunosorbent assay (ELISA) for specific detection of HA Ag in human stool filtrates and of anti-HA in sera by using selected HA Ag-containing human stool filtrates as the antigen source. Because human stools often react nonspecifically in serological tests for HA Ag, blocking with preexposure and hyperimmune anti-HA sera from a chimpanzee inoculated with hepatitis A virus was used to confirm specific detection of HA Ag. The sensitivity of ELISA was found to be comparable to that of solid-phase radioimmunoassay (SPRIA) and immune electron microscopy (IEM). Of 37 acute-phase stools collected from nine patients, 16 were positive for HA Ag by ELISA. In 13 of these, HA Ag particles were found by IEM, and an additional 3 stools negative by ELISA contained HA Ag particles by IEM. Eight control stools were negative by both ELISA and IEM. Anti-HA was measured in sera by demonstrating its ability to block binding of the enzyme conjugate to HA Ag in a stool without detectable nonspecificity. This test (blocking ELISA) was as sensitive and specific as blocking SPIRA, IEM, and immune adherence hemagglutination and, like SPRIA and IEM, detected early-developing antibody. The ELISA is simple to perform and requires only a minimum of equipment. It is useful for screening stools for HA Ag and for monitoring HA Ag during purification, as well as for detecting early and late anti-HA in sera.

Hepatitis A: Detection by Immune Electron Microscopy of a Viruslike Antigen Associated with Acute Illness

Science ◽  
1973 ◽  
Vol 182 (4116) ◽  
pp. 1026-1028 ◽  
Author(s):  
S. M. Feinstone ◽  
A. Z. Kapikian ◽  
R. H. Purcell
Keyword(s):  
Electron Microscopy ◽  
Hepatitis A ◽  
Acute Illness ◽  
Immune Electron Microscopy

IMMUNE ELECTRON MICROSCOPY AND HEPATITIS A

The Lancet ◽  
1975 ◽  
Vol 305 (7898) ◽  
pp. 102 ◽  
Author(s):  
JulesL Dienstag ◽  
StephenM Feinstone ◽  
AlbertZ Kapikian ◽  
RobertH Purcell
Keyword(s):  
Electron Microscopy ◽  
Hepatitis A ◽  
Immune Electron Microscopy

Comparison of Sensitivity of Radioimmunoassay and Immune Electron Microscopy for Detecting Hepatitis A Antigen in Fecal Extracts

1977 ◽  
Vol 155 (2) ◽  
pp. 193-198 ◽  
Author(s):  
W. T. Hall ◽  
D. W. Bradley ◽  
D. L. Madden ◽  
D. H. Zimmerman ◽  
D. E. L. Brandt
Keyword(s):  
Electron Microscopy ◽  
Hepatitis A ◽  
Immune Electron Microscopy
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