Guanidine Inhibits Tobacco Mosaic Virus RNA Synthesis at Two Stages

Intervirology ◽  
1975 ◽  
Vol 6 (2) ◽  
pp. 83-89 ◽  
Author(s):  
William O. Dawson
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Rna Synthesis ◽  
Virus Rna ◽  
Two Stages

Deletion analysis of the 5' untranslated leader sequence of tobacco mosaic virus RNA.

1991 ◽  
Vol 65 (3) ◽  
pp. 1619-1622 ◽  
Author(s):  
N Takamatsu ◽  
Y Watanabe ◽  
T Iwasaki ◽  
T Shiba ◽  
T Meshi ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Leader Sequence ◽  
Deletion Analysis ◽  
Virus Rna

scholarly journals Role of the 3′ tRNA-Like Structure in Tobacco Mosaic Virus Minus-Strand RNA Synthesis by the Viral RNA-Dependent RNA Polymerase In Vitro

2000 ◽  
Vol 74 (24) ◽  
pp. 11671-11680 ◽  
Author(s):  
T. A. M. Osman ◽  
C. L. Hemenway ◽  
K. W. Buck
Keyword(s):  
Rna Polymerase ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Untranslated Region ◽  
Rna Synthesis ◽  
Central Core ◽  
Minus Strand ◽  
Stem Loop ◽  
Polymerase Binding

ABSTRACT A template-dependent RNA polymerase has been used to determine the sequence elements in the 3′ untranslated region of tobacco mosaic virus RNA that are required for promotion of minus-strand RNA synthesis and binding to the RNA polymerase in vitro. Regions which were important for minus-strand synthesis were domain D1, which is equivalent to a tRNA acceptor arm; domain D2, which is similar to a tRNA anticodon arm; an upstream domain, D3; and a central core, C, which connects domains D1, D2, and D3 and determines their relative orientations. Mutational analysis of the 3′-terminal 4 nucleotides of domain D1 indicated the importance of the 3′-terminal CA sequence for minus-strand synthesis, with the sequence CCCA or GGCA giving the highest transcriptional efficiency. Several double-helical regions, but not their sequences, which are essential for forming pseudoknot and/or stem-loop structures in domains D1, D2, and D3 and the central core, C, were shown to be required for high template efficiency. Also important were a bulge sequence in the D2 stem-loop and, to a lesser extent, a loop sequence in a hairpin structure in domain D1. The sequence of the 3′ untranslated region upstream of domain D3 was not required for minus-strand synthesis. Template-RNA polymerase binding competition experiments showed that the highest-affinity RNA polymerase binding element region lay within a region comprising domain D2 and the central core, C, but domains D1 and D3 also bound to the RNA polymerase with lower affinity.

Nucleotide sequence and translation of satellite tobacco mosaic virus RNA

Virology ◽  
1989 ◽  
Vol 170 (1) ◽  
pp. 139-146 ◽  
Author(s):  
T.E. Mirkov ◽  
D.M. Mathews ◽  
D.H. Du Plessis ◽  
J.A. Dodds
Keyword(s):  
Nucleotide Sequence ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Rna ◽  
Satellite Tobacco Mosaic Virus

Methods for cDNA cloning and sequencing tobacco mosaic virus RNA

Gene ◽  
1984 ◽  
Vol 29 (3) ◽  
pp. 331-342 ◽  
Author(s):  
Philip Goelet ◽  
Jonathan Karn
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Cdna Cloning ◽  
Cloning And Sequencing ◽  
Virus Rna

Molecular cloning of the complementary DNA copies of the common and cowpea strains of tobacco mosaic virus RNA

Virology ◽  
1982 ◽  
Vol 118 (1) ◽  
pp. 64-75 ◽  
Author(s):  
Tetsuo Meshi ◽  
Nobuhiko Takamatsu ◽  
Takeshi Ohno ◽  
Yoshimi Okada
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Molecular Cloning ◽  
Complementary Dna ◽  
Virus Rna ◽  
The Common
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