Isolation of Field Rabies Virus Strains in CER and Murine Neuroblastoma Cell Cultures

Intervirology ◽  
1978 ◽  
Vol 9 (6) ◽  
pp. 359-361 ◽  
Author(s):  
Abigail L. Smith ◽  
Gregory H. Tignor ◽  
Richard W. Emmons ◽  
James D. Woodie
Keyword(s):  
Rabies Virus ◽  
Cell Cultures ◽  
Neuroblastoma Cell ◽  
Murine Neuroblastoma ◽  
Virus Strains

The apparent infection of NA-C1300 cell cultures by nucleocapsid material of the Canadian Arctic strain of rabies virus

1989 ◽  
Vol 35 (8) ◽  
pp. 811-813
Author(s):  
W. A. Webster ◽  
K. M. Charlton
Keyword(s):  
Rabies Virus ◽  
Cell Cultures ◽  
Virus Strain ◽  
Canadian Arctic ◽  
The Other ◽  
Baby Hamster Kidney ◽  
Bhk Cells ◽  
Murine Neuroblastoma ◽  
Other Hand

Murine neuroblastoma (NA-C1300) and baby hamster kidney (BHK-21/C13) cell cultures were infected with the Canadian Arctic strain of rabies virus. Subcultures were passed following incubation for 3 to 4 days at 35 °C. The supernatant fluids from the BHK cultures demonstrated increasing infectivity in both NA and BHK cells concomitantly with an increase in the number of parent cells staining with an anti-glycoprotein stain. On the other hand, the supernatant fluids from the NA cultures initially showed higher infectivity in NA cells than in BHK cells. This feature was related to a low production of glycoprotein-staining cells in the parent NA cultures. The reduction of infectivity in NA cells of some NA supernatant fluids (and brain suspensions) by anti-nucleoprotein antibodies suggests that nucleocapsid material is, in some manner, capable of infecting NA cells. Infectivity of this virus strain in experimental mice is also related to the production of glycoprotein and may not be correlated with the degree of infection in NA cell cultures.Key words: rabies, nucleocapsid, infection, cells.

scholarly journals Chronic Rabies Virus Infection of Cell Cultures

1972 ◽  
Vol 6 (6) ◽  
pp. 988-995 ◽  
Author(s):  
T. J. Wiktor ◽  
H F. Clark
Keyword(s):  
Virus Infection ◽  
Rabies Virus ◽  
Cell Cultures ◽  
Rabies Virus Infection

scholarly journals Impedance Study of Dopamine Effects after Application on 2D and 3D Neuroblastoma Cell Cultures Developed on a 3D-Printed Well

Chemosensors ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 6 ◽  
Author(s):  
Georgia Paivana ◽  
Theofylaktos Apostolou ◽  
Sophie Mavrikou ◽  
Dimitris Barmpakos ◽  
Grigoris Kaltsas ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Immobilized Cells ◽  
Neuroblastoma Cell ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Impedance Analysis ◽  
Neural Cells ◽  
Opposite Behavior ◽  
2D And 3D

In this work, the assessment of the interactions of a bioactive substance applied to immobilized cells in either a two-dimensional (2D) or three-dimensional (3D) arrangement mimicking in vivo tissue conditions is presented. In particular, dopamine (DA) was selected as a stimulant for the implementation of an impedance analysis with a specific type of neural cells (murine neuroblastoma). The aim of this study was the extraction of calibration curves at various frequencies with different known dopamine concentrations for the description of the behavior of dopamine applied to 2D and 3D cell cultures. The results present the evaluation of the mean impedance value for each immobilization technique in each frequency. The differential responses showed the importance of the impedance when frequency is applied in both 2D and 3D immobilization cases. More specifically, in 2D immobilization matrix impedance shows higher values in comparison with the 3D cell culture. Additionally, in the 3D case, the impedance decreases with increasing concentration, while in the 2D case, an opposite behavior was observed.

scholarly journals Ecology and evolution of rabies virus in Europe

1999 ◽  
Vol 80 (10) ◽  
pp. 2545-2557 ◽  
Author(s):  
Hervé Bourhy ◽  
Bachir Kissi ◽  
Laurent Audry ◽  
Marcin Smreczak ◽  
Malgorzata Sadkowska-Todys ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Sequence Data ◽  
Geographical Area ◽  
Red Foxes ◽  
Phylogenetic Groups ◽  
European Origin ◽  
Vistula River ◽  
Physical Barriers ◽  
Raccoon Dogs ◽  
Virus Strains

The evolution of rabies viruses of predominantly European origin was studied by comparing nucleotide sequences of the nucleoprotein and glycoprotein genes, and by typing isolates using RFLP. Phylogenetic analysis of the gene sequence data revealed a number of distinct groups, each associated with a particular geographical area. Such a pattern suggests that rabies virus has spread westwards and southwards across Europe during this century, but that physical barriers such as the Vistula river in Poland have enabled localized evolution. During this dispersal process, two species jumps took place – one into red foxes and another into raccoon dogs, although it is unclear whether virus strains are preferentially adapted to particular animal species or whether ecological forces explain the occurrence of the phylogenetic groups.

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