Fine Structure of the Epidermal Basement Membrane of the Lip: Applications of Dithiothreitol Separation and Ultrathin Frozen Sectioning

1995 ◽  
Vol 153 (2) ◽  
pp. 106-110 ◽  
Author(s):  
T. Osawa ◽  
Y. Nozaka
Keyword(s):  
Fine Structure ◽  
Basement Membrane ◽  
Epidermal Basement Membrane

scholarly journals THE FINE STRUCTURE OF THE BASEMENT MEMBRANE IN EPIDERMAL TUMORS

1962 ◽  
Vol 15 (2) ◽  
pp. 335-342 ◽  
Author(s):  
J. V. Frei
Keyword(s):  
Fine Structure ◽  
Basement Membrane ◽  
Potassium Permanganate ◽  
Topical Application ◽  
Osmium Tetroxide ◽  
Malignant Tumors ◽  
Female Mice ◽  
Lead Hydroxide ◽  
Repeated Applications ◽  
Epidermal Basement Membrane

Epidermal tumors were induced in Swiss female mice by a topical application of 9,10-dimethyl-1,2-benzanthracene solution followed by repeated applications of croton oil solutions. Fourteen benign and malignant tumors were sampled 25 weeks after the treatment had begun, fixed in osmium tetroxide or potassium permanganate, and embedded in Epon. Sections stained with lead hydroxide were examined. In three tumors defects of the epidermal basement membrane were seen. These defects accompanied local invasion of the tumors. The possible mechanisms of the development of this unusual anatomical situation are discussed.

scholarly journals Epiligrin, a component of epithelial basement membranes, is an adhesive ligand for alpha 3 beta 1 positive T lymphocytes.

1993 ◽  
Vol 121 (5) ◽  
pp. 1141-1152 ◽  
Author(s):  
E A Wayner ◽  
S G Gil ◽  
G F Murphy ◽  
M S Wilke ◽  
W G Carter
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Lymphocytes ◽  
Basement Membrane ◽  
B Cell ◽  
Cell Lines ◽  
B Cell Lymphoma ◽  
Lymphokine Activated Killer Cells ◽  
Cutaneous Inflammation ◽  
Epidermal Basement Membrane

The cutaneous T cell lymphomas (CTCL), typified by mycosis fungoides, and several chronic T cell mediated dermatoses are characterized by the migration of T lymphocytes into the epidermis (epidermotropism). Alternatively, other types of cutaneous inflammation (malignant cutaneous B cell lymphoma, CBCL, or lymphocytoma cutis, non-malignant T or B cell type) do not show evidence of epidermotropism. This suggests that certain T lymphocyte subpopulations are able to interact with and penetrate the epidermal basement membrane. We show here that T lymphocytes derived from patients with CTCL (HUT 78 or HUT 102 cells), adhere to the detergent-insoluble extracellular matrix prepared from cultured basal keratinocytes (HFK ECM). HUT cell adhesion to HFK ECM was inhibitable with monoclonal antibodies (mAbs) directed to the alpha 3 (P1B5) or beta 1 (P4C10) integrin receptors, and could be up-regulated by an activating anti-beta 1 mAb (P4G11). An inhibitory mAb, P3H9-2, raised against keratinocytes identified epiligrin as the ligand for alpha 3 beta 1 positive T cells in HFK ECM. Interestingly, two lymphocyte populations could be clearly distinguished relative to expression of alpha 3 beta 1 by flow cytometry analysis. Lymphokine activated killer cells, alloreactive cytotoxic T cells and T cells derived from patients with CTCL expressed high levels of alpha 3 beta 1 (alpha 3 beta 1high). Non-adherent peripheral blood mononuclear cells, acute T or B lymphocytic leukemias, or non-cutaneous T or B lymphocyte cell lines expressed low levels of alpha 3 beta 1 (alpha 3 beta 1low). Resting PBL or alpha 3 beta 1low T or B cell lines did not adhere to HFK ECM or purified epiligrin. However, adhesion to epiligrin could be up-regulated by mAbs which activate the beta 1 subunit indicating that alpha 3 beta 1 activity is a function of expression and affinity. In skin derived from patients with graft-vs.-host (GVH) disease, experimentally induced delayed hypersensitivity reactions, and CTCL, the infiltrating T cells could be stained with mAbs to alpha 3 or beta 1 and were localized in close proximity to the epiligrin-containing basement membrane. Infiltrating lymphocytes in malignant cutaneous B disease (CBCL) did not express alpha 3 beta 1 by immunohistochemical techniques and did not associate with the epidermal basement membrane. The present findings clearly define a function for alpha 3 beta 1 in T cells and strongly suggest that alpha 3 beta 1 interaction with epiligrin may be involved in the pathogenesis of cutaneous inflammation.

scholarly journals Interplay between Cell-Surface Receptors and Extracellular Matrix in Skin

Biomolecules ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1170
Author(s):  
Svenja Kleiser ◽  
Alexander Nyström
Keyword(s):  
Extracellular Matrix ◽  
Basement Membrane ◽  
Cell Surface ◽  
Skin Diseases ◽  
Biochemical Properties ◽  
Dermal Fibroblasts ◽  
Cell Surface Receptors ◽  
Surface Receptors ◽  
Dermal Cell ◽  
Epidermal Basement Membrane

Skin consists of the epidermis and dermis, which are connected by a specialized basement membrane—the epidermal basement membrane. Both the epidermal basement membrane and the underlying interstitial extracellular matrix (ECM) created by dermal fibroblasts contain distinct network-forming macromolecules. These matrices play various roles in order to maintain skin homeostasis and integrity. Within this complex interplay of cells and matrices, cell surface receptors play essential roles not only for inside-out and outside-in signaling, but also for establishing mechanical and biochemical properties of skin. Already minor modulations of this multifactorial cross-talk can lead to severe and systemic diseases. In this review, major epidermal and dermal cell surface receptors will be addressed with respect to their interactions with matrix components as well as their roles in fibrotic, inflammatory or tumorigenic skin diseases.

scholarly journals 631 Laminin-511 is a key component of epidermal basement membrane to maintain epidermal homeostasis

2018 ◽  
Vol 138 (5) ◽  
pp. S107
Author(s):  
S. Iriyama ◽  
S. Nishikawa ◽  
E. Takai ◽  
H. Yamanishi ◽  
N. Kunizawa ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Epidermal Homeostasis ◽  
Epidermal Basement Membrane

scholarly journals The Fine Structure of Some Blood Vessels of the Earthworm, Eisenia foetida

1960 ◽  
Vol 7 (4) ◽  
pp. 717-724 ◽  
Author(s):  
Kiyoshi Hama
Keyword(s):  
Fine Structure ◽  
Basement Membrane ◽  
Body Wall ◽  
Circular Muscle ◽  
Muscle Layer ◽  
Eisenia Foetida ◽  
Longitudinal Muscle Layer ◽  
Cell Processes ◽  
Body Wall Musculature ◽  
Blood Pigment

The fine structure of the main dorsal and ventral circulatory trunks and of the subneural vessels and capillaries of the ventral nerve cord of the earthworm, Eisenia foetida, has been studied with the electron microscope. All of these vessels are lined internally by a continuous extracellular basement membrane varying in thickness (0.03 to 1 µ) with the vessel involved. The dorsal, ventral, and subneural vessels display inside this membrane scattered flattened macrophagic or leucocytic cells called amebocytes. These lie against the inner lining of the basement membrane, covering only a small fraction of its surface. They have long, attenuated branching cell processes. All of these vessels are lined with a continuous layer of unfenestrated endothelial cells displaying myofilaments and hence qualifying for the designation of "myoendothelial cells." The degree of muscular specialization varies over a spectrum, however, ranging from a delicate endowment of thin myofilaments in the capillary myoendothelial cells to highly specialized myoendothelial cells in the main pulsating dorsal blood trunk, which serves as the worm's "heart" or propulsive "aorta." The myoendothelial cells most specialized for contraction display well organized sarcoplasmic reticulum and myofibrils with thick and thin myofilaments resembling those of the earthworm body wall musculature. In the ventral circulatory trunk, circular and longitudinal myofilaments are found in each myoendothelial cell. In the dorsal trunk, the lining myoendothelial cells contain longitudinal myofilaments. Outside these cells are circular muscle cells. The lateral parts of the dorsal vessels have an additional outer longitudinal muscle layer. The blood plasma inside all of the vessels shows scattered particles representing the circulating earthworm blood pigment, erythrocruorin.

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