Ultrastructural Observation of Calcification Preceding New Bone Formation Induced by Demineralized Bone Matrix Gelatin

1992 ◽  
Vol 143 (4) ◽  
pp. 261-267 ◽  
Author(s):  
K. Yamashita ◽  
T. Takagi
Keyword(s):  
Bone Formation ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Ultrastructural Observation ◽  
New Bone Formation ◽  
Demineralized Bone

Tissue Engineering of Bone by Osteoinductive Biomaterials

MRS Bulletin ◽  
1996 ◽  
Vol 21 (11) ◽  
pp. 36-39 ◽  
Author(s):  
Ugo Ripamonti ◽  
Nicolaas Duneas
Keyword(s):  
Tissue Engineering ◽  
Bone Formation ◽  
Type I Collagen ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Mesenchymal Cells ◽  
Tissue Response ◽  
Type I ◽  
New Bone Formation ◽  
Demineralized Bone

Recent advances in materials science and biotechnology have given birth to the new and exciting field of tissue engineering, in which the two normally disparate fields are merging into a profitable matrimony. In particular the use of biomaterials capable of initiating new bone formation via a process called osteoinduction is leading to quantum leaps for the tissue engineering of bone.The classic work of Marshall R. Urist and A. Hari Reddi opened the field of osteoinductive biomaterials. Urist discovered that, upon implantation of devitalized, demineralized bone matrix in the muscle of experimental animals, new bone formation occurs within two weeks, a phenomenon he described as bone formation by induction. The tissue response elicited by implantation of demineralized bone matrix in muscle or under the skin includes activation and migration of undifferentiated mesenchymal cells by chemotaxis, anchoragedependent cell attachment to the matrix, mitosis and proliferation of mesenchymal cells, differentiation of cartilage, mineralization of the cartilage, vascular invasion of the cartilage, differentiation of osteoblasts and deposition of bone matrix, and finally mineralization of bone and differentiation of marrow in the newly developed ossicle.The osteoinductive ability of the extracellular matrix of bone is abolished by the dissociative extraction of the demineralized matrix, but is recovered when the extracted component, itself inactive, is reconstituted with the inactive residue—mainly insoluble collagenous bone matrix. This important experiment showed that the osteoinductive signal resides in the solubilized component but needs to be reconstituted with an appropriate carrier to restore the osteoinductive activity. In this case, the carrier is the insoluble collagenous bone matrix—mainly crosslinked type I collagen.

Effect of Bupivacaine on Muscle Tissues and New Bone Formation Induced by Demineralized Bone Matrix Gelatin

1991 ◽  
Vol 141 (1) ◽  
pp. 1-7 ◽  
Author(s):  
K. Yamashita ◽  
Y. Horisaka ◽  
Y. Okamoto ◽  
Y. Yoshimura ◽  
N. Matsumoto ◽  
...  
Keyword(s):  
Bone Formation ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
New Bone Formation ◽  
Muscle Tissues ◽  
Demineralized Bone

scholarly journals Demineralized Bone Matrix Coating Si-Ca-P Ceramic Does Not Improve the Osseointegration of the Scaffold

Materials ◽  
2018 ◽  
Vol 11 (9) ◽  
pp. 1580 ◽  
Author(s):  
Andrés Parrilla-Almansa ◽  
Nuria García-Carrillo ◽  
Patricia Ros-Tárraga ◽  
Carlos Martínez ◽  
Francisco Martínez-Martínez ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Tissue ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Micro Ct ◽  
New Bone Formation ◽  
Implantation Time ◽  
Post Surgery ◽  
Tissue Responses ◽  
Demineralized Bone

The aim of this study was to manufacture and evaluate the effect of a biphasic calcium silicophosphate (CSP) scaffold ceramic, coated with a natural demineralized bone matrix (DBM), to evaluate the efficiency of this novel ceramic material in bone regeneration. The DBM-coated CSP ceramic was made by coating a CSP scaffold with gel DBM, produced by the partial sintering of different-sized porous granules. These scaffolds were used to reconstruct defects in rabbit tibiae, where CSP scaffolds acted as the control material. Micro-CT and histological analyses were performed to evaluate new bone formation at 1, 3, and 5 months post-surgery. The present research results showed a correlation among the data obtained by micro-CT and the histomorphological results, the gradual disintegration of the biomaterial, and the presence of free scaffold fragments dispersed inside the medullary cavity occupied by hematopoietic bone marrow over the 5-month study period. No difference was found between the DBM-coated and uncoated implants. The new bone tissue inside the implants increased with implantation time. Slightly less new bone formation was observed in the DBM-coated samples, but it was not statistically significant. Both the DBM-coated and the CSP scaffolds gave excellent bone tissue responses and good osteoconductivity.

Serum albumin coating of demineralized bone matrix results in stronger new bone formation

2015 ◽  
Vol 104 (1) ◽  
pp. 126-132 ◽  
Author(s):  
Dénes B. Horváthy ◽  
Gabriella Vácz ◽  
Tamás Szabó ◽  
Imola C. Szigyártó ◽  
Ildikó Toró ◽  
...  
Keyword(s):  
Bone Formation ◽  
Serum Albumin ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
New Bone Formation ◽  
Demineralized Bone

scholarly journals Natural variation in the extent of phosphorylation of bone phosphoproteins as a function of in vivo new bone formation induced by demineralized bone matrix in soft tissue and bony environments

2002 ◽  
Vol 364 (2) ◽  
pp. 465-474 ◽  
Author(s):  
Erdjan SALIH ◽  
Jinxi WANG ◽  
James MAH ◽  
Rudolf FLUCKIGER
Keyword(s):  
Soft Tissue ◽  
Bone Formation ◽  
Natural Variation ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Anatomical Location ◽  
New Bone Formation ◽  
First Time ◽  
Demineralized Bone

Implants of allogenic demineralized bone matrix were placed in distinct in vivo environments, i.e. calvarial (bony) and subcutaneous (soft tissue) sites. Detailed analyses of the biochemical components were performed. Quantitative levels of osteopontin (OPN), bone sialoprotein (BSP) and calcium phosphate (Ca-P) deposition within each implant environment varied as a function of new bone formation, and were substantially different in samples from calvarial and subcutaneous sites. Quantification of the extent of phosphorylation of affinity-purified OPN and BSP from such implants indicated that: (i) the number of mols of phosphoserine (P-Ser)/mol of affinity-purified OPN or BSP varied as a function of implant time and bone formation within both implant sites, and (ii) the ‘effective P-Ser concentration’ provided by the total OPN and BSP within each implant site varied and increased as a function of time, being approx. 5-fold higher for BSP in calvarial compared with subcutaneous implants. Peak levels of mols of P-Ser/mol of BSP coincided with maximum rates of Ca-P deposition in calvarial implants. Levels of OPN phosphorylation from both calvarial and subcutaneous implants also indicated fluctuations as a function of bone formation. Hence the present study, for the first time, provides direct evidence of natural variation in the extent of phosphorylation of both OPN and BSP as a function of time of mineralized tissue formation. Further evaluation of the data provides the first evidence of a direct and linear relationship between the rate of Ca-P deposition and the ratio of P-Ser-BSP/P-Ser-OPN for calvarial implants. Data for subcutaneous implants failed to provide such correlation. Overall, the present work demonstrates that the natural biological progression of the process of biomineralization follows strict criteria consistent with the anatomical location. Biomineralization fails to proceed in the same way in a soft tissue environment.

Platelet-Rich Plasma Inhibits Demineralized Bone Matrix-Induced Bone Formation in Nude Mice

2007 ◽  
Vol 89 (1) ◽  
pp. 139-147 ◽  
Author(s):  
Don M. Ranly ◽  
Barbara D. Boyan ◽  
Zvi Schwartz ◽  
Christoph H. Lohmann ◽  
Domenico Andreacchio
Keyword(s):  
Bone Formation ◽  
Nude Mice ◽  
Demineralized Bone Matrix ◽  
Platelet Rich Plasma ◽  
Bone Matrix ◽  
Demineralized Bone
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