Architecture of the Myosalpinx of the Isthmus in the Guinea Pig by Means of Scanning Electron Microscopy

1991 ◽  
Vol 142 (2) ◽  
pp. 171-173 ◽  
Author(s):  
U. Muglia ◽  
E. Vizza ◽  
S. Correr ◽  
G. Germanà ◽  
P.M. Motta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Guinea Pig ◽  
Scanning Electron

Scanning and Transmission Electron Microscopic correlates of sulfur mustard (HD) toxicity

1989 ◽  
Vol 47 ◽  
pp. 1098-1099
Author(s):  
J.P. Petrali ◽  
S.B. Oglesby ◽  
K.R. Mills
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Guinea Pig ◽  
Human Skin ◽  
Sulfur Mustard ◽  
Pig Model ◽  
Transmission Electron ◽  
Hairless Guinea Pig ◽  
Guinea Pig Model ◽  
Scanning Electron

In spite of the many reports, since World War I no the pathology of mustard injury, no ultrastructural studies were performed until our laboratory published a report on the ultrastructure of the pathogenesis of blister formation following exposure to sulfur mustard of human–skin grafted to congenitally athymic nude mice. This study afforded us the opportunity for the first time to focus on several objectives: 1) to further delineate the histopathology which had been noted at light microscopy levels; 2) to identify possible early mustard-induced morpho- logical changes which may occur during the latent asynptcamtic phase; and 3) to prcmote our understanding of the temporal features of mustard pathology with the expectation that prophylactic and therapeutic strategies might be morphologically predictable. We now have the opportunity to describe HD-induced pathology in cells-in-culture and compare them with that of the skin lesion of a human skin grafted model and a hairless guinea pig model.We used standardized ultrastructural technology to study by transmission and scanning electron microscopy the subcellular effects of HD on human skin grafted to athymic mice, human lyrrphocytes and human keratinocytes in culture, and, the hairless guinea pig model. While doses of mustard differed according to the dictates of individualized protocols, all specimens were gathered at 24-48 hours following exposure and were aldehyde fixed, dehydrated in graded ethanols and either embedded in epoxy resin for transmission electron microscopy or critical-point dried and sputter coated with gold-palladium for scanning electron microscopy.

Scanning electron microscopy of the three different types of cementum in the molar teeth of the guinea pig

2006 ◽  
Vol 51 (6) ◽  
pp. 439-448 ◽  
Author(s):  
Keita Moriyama ◽  
Noriyuki Sahara ◽  
Toru Kageyama ◽  
Yasuko Misawa ◽  
Akihiro Hosoya ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Guinea Pig ◽  
Different Types ◽  
Molar Teeth ◽  
Scanning Electron

Sperm associations in rat epididymis

1990 ◽  
Vol 48 (3) ◽  
pp. 56-57
Author(s):  
Miguel W. Fomés ◽  
Mario H. Burgos
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Guinea Pig ◽  
Light Microscopy ◽  
Salt Solution ◽  
Transmission Electron ◽  
Rat Epididymis ◽  
Cover Slide ◽  
Scanning Electron

During epididymal transit a head to head association of spermatozoa has been described in guinea pig, Loris and marsupials. In guinea pig they are permanent and androgen dependent. Other type of sperm association described in nonseasonal. mammals, adopt the stiape of dense masses of degenerating spermatozoa.In the present study a new transitory head to head association in rat spemiatozoa is described which appears at the end of the epididymal transit and dense masses of associated spennatozoa in degeneration similar to those described in the literature. The sperm samples were obtained by puncture of the 3 main epididymal regions. The emerging fluid drops were immediately suspended in a balanced salt solution, placed in a slide-cover slide chanber at 36°C, and observed and recorded with a video equipment. Other drops of epididymal fluid were fixed in Mollenhauer (1976) and processed for light microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM).

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