Consistently Nonoverlapping Distribution of Epidermal Growth Factor Receptors in Adult Human Skin Detected by Various Monoclonal Antibodies

1990 ◽  
Vol 137 (3) ◽  
pp. 202-207 ◽  
Author(s):  
Yuko Misumi ◽  
Toshio Akiyoshi
Keyword(s):  
Monoclonal Antibodies ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Human Skin ◽  
Growth Factor Receptors ◽  
Epidermal Growth Factor Receptors ◽  
Adult Human ◽  
Epidermal Growth ◽  
Adult Human Skin

Immunolocalization of Epidermal Growth Factor Receptors in Normal Developing Human Skin

1990 ◽  
Vol 94 (6) ◽  
pp. 742-748 ◽  
Author(s):  
Lillian B. Nanney ◽  
Christa M. Stoscheck ◽  
Robert A. Underwood ◽  
Karen A. Holbrook ◽  
Lloyd E. King
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Human Skin ◽  
Growth Factor Receptors ◽  
Epidermal Growth Factor Receptors ◽  
Epidermal Growth

Monoclonal antibodies to epidermal growth factor receptors in studies of receptor structure and function

1990 ◽  
Vol 3 (3) ◽  
pp. 279-293 ◽  
Author(s):  
Tomoyuki Kawamoto ◽  
Gordon H. Sato ◽  
Kojiro Takahashi ◽  
Mieko Nishi ◽  
Shigehiko Taniguchi ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Growth Factor Receptors ◽  
Structure And Function ◽  
Epidermal Growth Factor Receptors ◽  
Receptor Structure ◽  
Epidermal Growth ◽  
And Function

UVB radiation affects the mobility of epidermal growth factor receptors in human keratinocytes and fibroblasts

1996 ◽  
Vol 16 (3) ◽  
pp. 227-238 ◽  
Author(s):  
Margareta Lirvall ◽  
Pia Ljungqvist-Höddelius ◽  
Åke Wasteson ◽  
Karl-Eric Magnùsson
Keyword(s):  
Antioxidant Enzymes ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Human Skin ◽  
Fluorescence Recovery After Photobleaching ◽  
Growth Factor Receptors ◽  
Human Keratinocytes ◽  
Epidermal Growth Factor Receptors ◽  
Uvb Radiation ◽  
Epidermal Growth

Growth factor receptors transmit biological signals for the stimulation of cell growth in vitro and in vivo and their autocrine stimulation may be involved in tumorigenesis. It is therefore, of great value to understand receptor reactions in response to ultraviolet (UV) light which certain normal human cells are invaribly exposed to during their growth cycle. UV irradiation has recently been shown to deplete antioxidant enzymes in human skin. The aims of the present study were a) to compare the lateral mobility of epidermal growth factor receptors (EGF-R) in cultured human keratinocytes and human foreskin fibroblasts, b) to investigate effects of ultraviolet B radiation on the mobility of EGF-R in these cells, and c) study the response of EGF-R on addition of antioxidant enzymes. The epidermal growth factor receptors were labeled with rhodaminated EGF, the lateral diffusion was determined and the fraction of mobile EGF-R assessed with the fluorescence recovery after photobleaching (FRAP). We found that human keratinocytes display a higher basal level of EGF-R mobility than human skin fibroblasts, viz. with diffusion coefficients (D ± standard error of the mean, SEM) of 4.2±0.2 × 10−10 cm2/s, and 1.8±0.2 × 10−10 cm2/s, respectively. UVB-irradiated fibroblasts showed an almost four-fold increase in the diffusion coefficient; D was 6.3±0.3 × 10−10 cm2/s. The keratinocytes, however, displayed no significant increase in receptor diffusion after irradiation; D was 5.1±0.8 × 10−10 cm2/s. In both cell types the percentage of EGF-R fluorescence recovery after photobleaching, i.e. the fraction of mobile receptors, was significantly increased after irradiation. In keratinocytes it increased from 69% before irradiation to 78% after irradiation. Analogous figures for fibroblasts were 61% and 73%. The effect of UVB on fibroblast receptors was abolished by prior addition of superoxide dismutase (SOD) and catalase (CAT). It is concluded that UVB radiation of fibroblasts and keratinocytes can affect their biophysical properties of EGF-R. The finding that addition of antioxidant enzymes prevented the UVB effect in fibroblasts may indicate the involvement of reactive oxygen metabolites.

Sign in / Sign up

Export Citation Format

Share Document