Change in Structure of Tight Junctions between Epithelial Cells in the Uterine Tube of the Rat during Early Pregnancy

1988 ◽  
Vol 132 (3) ◽  
pp. 253-255 ◽  
Author(s):  
C.R. Murphy ◽  
V.F. Turner
Keyword(s):  
Epithelial Cells ◽  
Tight Junctions ◽  
Early Pregnancy ◽  
Uterine Tube

238. Claudins and occludin in the rat endometrium

2005 ◽  
Vol 17 (9) ◽  
pp. 94
Author(s):  
M. D. O. Nicholson ◽  
C. R. Murphy
Keyword(s):  
Epithelial Cells ◽  
Tight Junctions ◽  
Early Pregnancy ◽  
Immunofluorescence Microscopy ◽  
Ovarian Hormones ◽  
Cellular Distribution ◽  
Luminal Epithelium ◽  
Uterine Lumen ◽  
Uterine Glands ◽  
Ovariectomised Rats

Regulation of the uterine luminal environment is important for the successful attachment and implantation of the blastocyst. Tight junctions regulate the paracellular pathway between epithelial cells lining the uterine lumen and the uterine glands. The aims of this present study was firstly to establish the presence and cellular distribution of claudins and occludin in the luminal epithelia during early pregnancy using immunofluorescence microscopy and deconvolution, and secondly to determine the influence of ovarian hormones on their expression. Occludin and claudins -1, -3, and -4 were present in luminal epithelium. Occludin and claudin-4 showed increased expression in luminal epithelium at the time of implantation, whereas claudin-1 and -3 expression remained the same throughout early pregnancy. In ovariectomised rats administered ovarian hormones, occludin and claudin-4 showed increased expression in luminal epithelium in progesterone-dominant regimes and decreased expression when administered oestrogen alone. Expression of claudin-1 and -3 in luminal epithelium was not effected by ovarian hormones. Claudin-2 was not expressed during early pregnancy nor in ovariectomised rats. In conclusion, these results show that occludin and claudins -1, -3 and -4 are present in luminal and glandular epithelium, and provide the permeability properties needed to separate the luminal and the stromal environment at the time of implantation. Furthermore, occludin and claudin-4 expression is controlled by ovarian hormones being upregulated by progesterone.

Tight Junctions of Human Uterine Epithelial Cells Change during the Menstrual Cycle: A Morphometric Study

1992 ◽  
Vol 144 (1) ◽  
pp. 36-38 ◽  
Author(s):  
C.R. Murphy ◽  
P.A.W. Rogers ◽  
M.J. Hosie ◽  
J. Leeton ◽  
L. Beaton
Keyword(s):  
Menstrual Cycle ◽  
Epithelial Cells ◽  
Tight Junctions ◽  
Morphometric Study ◽  
Uterine Epithelial Cells

The effect of berberine in vitro on tight junctions in human Caco-2 intestinal epithelial cells

Fitoterapia ◽  
2009 ◽  
Vol 80 (4) ◽  
pp. 241-248 ◽  
Author(s):  
Lili Gu ◽  
Ning Li ◽  
Qiurong Li ◽  
Qiang Zhang ◽  
Chengyang Wang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Tight Junctions ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial

Immunolocalization of sex steroid hormone receptors in the canine uterine tube and their relation to sex steroid hormone levels

2002 ◽  
Vol 14 (4) ◽  
pp. 241 ◽  
Author(s):  
Hilde Vermeirsch ◽  
Wim Van Den Broeck ◽  
Mark Coryn ◽  
Paul Simoens
Keyword(s):  
Epithelial Cells ◽  
Estrous Cycle ◽  
Stromal Cells ◽  
Hormone Receptors ◽  
Steroid Hormone ◽  
Steroid Hormone Receptors ◽  
Sex Steroid ◽  
Nuclear Staining ◽  
Sex Steroid Hormone ◽  
Uterine Tube

The aim of this immunohistochemical study was to describe the cellular distribution of the estrogen receptor-α (ERα), progesterone receptor (PR) and androgen receptor (AR) in canine uterine tubes. Samples of uterine tubes were taken from dogs in different stages of the estrous cycle, and dogs that were pregnant or had just delivered. Nuclear staining for sex steroid hormone receptors was observed in the surface epithelium, stromal cells and smooth muscle cells of the muscular layer. Only slight differences in staining pattern were observed between the ampulla and fimbriae. The staining for ERα and PR showed changes throughout the estrous cycle. Some of these changes were related to changing concentrations of sex steroid hormones. High staining scores for ERα and PR were found during proestrus and low scores during early metestrus. The staining for AR showed only minor cyclic changes. However, during proestrus and estrus, cytoplasmic staining for AR was observed in differentiated secretory epithelial cells, when nuclear staining in these cells was nearly absent. For the three hormone receptors, stromal cells generally stained with a higher intensity than epithelial cells. It is likely that many steroid hormone actions on the epithelium are mediated through stromal cells. During pregnancy, rather high staining scores were found for ERα and AR in the uterine tube. This is in contrast to observations in the canine pregnant uterus.

Impaired Localization of Claudin-11 in Endometriotic Epithelial Cells Compared to Endometrial Cells

2018 ◽  
Vol 26 (9) ◽  
pp. 1181-1192 ◽  
Author(s):  
Fabian Horné ◽  
Raimund Dietze ◽  
Eniko Berkes ◽  
Frank Oehmke ◽  
Hans-Rudolf Tinneberg ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Tight Junctions ◽  
Cell Lines ◽  
Human Cancer ◽  
Deep Infiltrating Endometriosis ◽  
Endometrial Cell ◽  
Ovarian Endometriosis ◽  
Eutopic Endometrium ◽  
Endometrial Cells ◽  
Ectopic Endometrium

Claudins are the major components of tight junctions and are often deregulated in human cancer, permitting escape of cancer cells along with the acquisition of invasive properties. Similarly, endometrial cells also show invasive capabilities; however, the role of tight junctions in endometriosis has only rarely been examined. In this study, we analyzed the protein expression and localization of claudin-7 and claudin-11 in human eutopic and ectopic endometrium and endometrial cell lines. We identified claudin-7 primarily at the basolateral junctions of the glandular epithelial cells in eutopic endometrium as well as in the ectopic lesions in nearly all glands and cysts. Quantification of claudin-7 localization by HSCORE showed a slight increase in peritoneal and deep infiltrating endometriosis (DIE) compared to eutopic endometrium. In contrast, claudin-11 was localized mainly in the apicolateral junctions in nearly all glandular epithelial cells of the eutopic endometrium. Interestingly, we observed a deregulation of claudin-11 localization to a basal or basolateral localization in ovarian ( P < .001), peritoneal ( P < .01), and DIE ( P < .05) and a moderately decreased abundance in ovarian endometriosis. In endometrial cell lines, claudin-7 was only present in epithelial Ishikawa cells, and silencing by small-interfering RNA increased cell invasiveness. In contrast, claudin-11 could be demonstrated in Ishikawa and endometriotic 12Z and 49Z cells. Silencing of claudin-11 decreased invasiveness of 12Z slightly but significantly in 49Z. We suggest that although claudin-7 and claudin-11 can be found in nearly all eutopic and ectopic epithelial cells, the impaired localization of claudin-11 in ectopic endometrium might contribute to the pathogenesis of endometriosis.

scholarly journals Mechanosensitive calcium signaling promotes epithelial tight junction remodeling by activating RhoA

2021 ◽  
Author(s):  
Saranyaraajan Varadarajan ◽  
Rachel E. Stephenson ◽  
Eileen R. Misterovich ◽  
Jessica L. Wu ◽  
Ivan S. Erofeev ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Intracellular Calcium ◽  
Tight Junctions ◽  
Calcium Influx ◽  
Cell Junctions ◽  
Mechanical Stimuli ◽  
Local Repair ◽  
Transient Activation ◽  
Epithelial Tight Junction ◽  
Sense And Respond

Epithelia maintain an effective barrier by remodeling cell-cell junctions in response to mechanical stimuli. Cells often respond to mechanical stress through activating RhoA and remodeling actomyosin. Previously, we found that local leaks in the barrier are rapidly repaired by localized, transient activation of RhoA – ″Rho flares″ – but how Rho flares are initiated remains unknown. Here, we discovered that intracellular calcium flashes occur in Xenopus laevis epithelial cells undergoing Rho flare-mediated remodeling of tight junctions. Calcium flashes originate at the site of barrier leaks and propagate into the cell. Depletion of intracellular calcium or inhibition of mechanosensitive calcium channels (MSC) reduced the amplitude of calcium flashes and diminished the activation of Rho flares. Furthermore, MSC-dependent calcium influx was necessary to maintain global barrier function by regulating local repair of tight junctions through efficient junction contraction. We propose that MSC-dependent calcium flashes are an important mechanism allowing epithelial cells to sense and respond to local leaks induced by mechanical stimuli.

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