The Effects of High-Sucrose Diets and of Maternal Diabetes on the Ultrastructure of the Visceral Yolk Sac Endoderm in Rat Embryos Developing in vivo and in vitro

1987 ◽  
Vol 128 (1) ◽  
pp. 11-18 ◽  
Author(s):  
I. Zusman ◽  
P. Yaffe ◽  
A. Ornoy
Keyword(s):  
Maternal Diabetes ◽  
Yolk Sac ◽  
Rat Embryos ◽  
Visceral Yolk Sac ◽  
High Sucrose

Effect of yolk-sac antibody on rat embryos grown in culture

Development ◽  
1972 ◽  
Vol 27 (3) ◽  
pp. 543-553
Author(s):  
D. A. T. New ◽  
R. L. Brent
Keyword(s):  
Direct Contact ◽  
Culture Medium ◽  
Gamma Globulin ◽  
Yolk Sac ◽  
Amniotic Cavity ◽  
Pregnant Rats ◽  
Rat Embryos ◽  
Visceral Yolk Sac ◽  
Primary Action

Rat embryos, explanted with their embryonic membranes during the early stages of organogenesis ( days gestation), were grown in culture in roller tubes. Yolk-sac antibody (sheep anti rat yolk-sac gamma globulin), known to be teratogenic when injected into pregnant rats, was added to the culture medium. At concentrations of 0·1 mg/ml or more the antibody caused gross retardation of growth and differentiation. Injection of antibody into the amniotic cavity so that it had direct contact with the embryo, or between the amnion and yolk sac so that it was in contact with the mesodermal surface of the yolk sac, had little or no effect on development of the embryo or its membranes. These in vitro experiments indicate that yolk-sac antibody has an effect on development independent of any immunological reaction of the mother, and the primary action is probably on the visceral yolk-sac endoderm.

Maternal diabetes in vivo and high glucose concentration in vitro increases apoptosis in rat embryos

2007 ◽  
Vol 23 (1) ◽  
pp. 63-74 ◽  
Author(s):  
Mattias Gäreskog ◽  
Jonas Cederberg ◽  
Ulf J. Eriksson ◽  
Parri Wentzel
Keyword(s):  
High Glucose ◽  
Glucose Concentration ◽  
Maternal Diabetes ◽  
High Glucose Concentration ◽  
Rat Embryos

scholarly journals Multiangle light-scattering analysis of murine teratocarcinoma cells.

1979 ◽  
Vol 27 (1) ◽  
pp. 366-370 ◽  
Author(s):  
D E Swartzendruber ◽  
B J Price ◽  
L B Rall
Keyword(s):  
Stem Cells ◽  
Light Scattering ◽  
Flow System ◽  
Yolk Sac ◽  
Squamous Cells ◽  
Teratocarcinoma Cells ◽  
Visceral Yolk Sac ◽  
Yolk Sac Cells

Stem cells of the mouse testicular teratocarcinoma are capable of giving rise in vivo and in vitro to a wide variety of cell and tissue types representative of each embryonic germ layer. Multiangle light-scattering measurements in a flow system have been made on these stem cells and on a variety of their differentiated derivatives. This technique is capable of distinguishing the stem cells from parietal yolk sac cells, visceral yolk sac cells, neuronal cells and squamous cells. However, multipotential stem cells cannot be distinguished from stem cells that are restricted in their development to a single pathway.

Differentiation potentiality of rat visceral yolk sac in organ culture

Development ◽  
1984 ◽  
Vol 80 (1) ◽  
pp. 127-136
Author(s):  
Y. L. Lu ◽  
H. Sobis ◽  
L. Van Hove ◽  
M. Vandeputte
Keyword(s):  
Organ Culture ◽  
In Vitro Culture ◽  
Yolk Sac ◽  
Trophoblast Cells ◽  
Differentiated Cells ◽  
Visceral Yolk Sac ◽  
Poorly Differentiated

Visceral yolk sacs removed at day 12 of pregnancy in the rat were kept in organ culture for as long as 28 days. During this in vitro culture, proliferation of the endoderm and the mesoderm as well as of poorly differentiated cells was observed. The latter displayed neither the characteristics of endodermal nor mesodermal cells and their presence was frequently associated with the development of giant trophoblast cells. The hypothesis is proposed that these trophoblast cells originate from these poorly differentiated cells that acquire in vivo and in vitro the potentiality to differentiate.

Changes in visceral yolk sac ultrastructure after exposure of rat embryos to selected teratogens in vitro

1990 ◽  
Vol 4 (4-5) ◽  
pp. 593-597 ◽  
Author(s):  
G.P. Daston ◽  
J.L. Burns ◽  
M.H. Chestnut
Keyword(s):  
Yolk Sac ◽  
Rat Embryos ◽  
Visceral Yolk Sac

scholarly journals Morphometric analysis of the visceral yolk sac endoderm in the rat in vivo and in vitro

Reproduction ◽  
1982 ◽  
Vol 65 (1) ◽  
pp. 239-245 ◽  
Author(s):  
M. Gupta ◽  
A.P. Gulamhusein ◽  
F. Beck
Keyword(s):  
Morphometric Analysis ◽  
Yolk Sac ◽  
Visceral Yolk Sac

Hepoxilin A, hydroxyepoxide metabolite of arachidonic acid, stimulates transport of 45Ca across the guinea pig visceral yolk sac

1984 ◽  
Vol 62 (12) ◽  
pp. 1466-1469 ◽  
Author(s):  
L. O. Derewlany ◽  
C. R. Pace-Asciak ◽  
I. C. Radde
Keyword(s):  
Arachidonic Acid ◽  
Guinea Pig ◽  
Calcium Transport ◽  
Yolk Sac ◽  
Maximal Effect ◽  
Isolated Pancreatic Islets ◽  
Ussing Chambers ◽  
Visceral Yolk Sac

The effect of hepoxilin A, a newly isolated hydroxyepoxide metabolite of arachidonic acid, on calcium transport across the visceral yolk sac membrane of the guinea pig was investigated in vitro in Ussing chambers. While 1-14C-labelled hepoxilin A itself was not transported across the membrane, it increased the rate of transport of calcium toward the side to which hepoxilin A was added. The degree of increase in calcium transport was similar whether hepoxilin A was added to the maternal side or to the fetal side of the membrane. The observed effect was dependent on the concentration of hepoxilin A over a narrow range (0.5–1.0 × 10−6 M). It was also dependent on the time of incubation reaching maximal effect by 25 min. We have recently observed that hepoxilin A is formed from platelet-derived 12-hydroperoxyeicosatetraenoic acid (12-HPETE) through hernin and hemoglobin catalysis as well as during perifusion of 12-HPETE through isolated pancreatic islets. The present study suggests that hepoxilin A, if formed in vivo, could play a role in the mobilization of calcium.

scholarly journals Maternal Diabetes In Vivo and High Glucose In Vitro Diminish GAPDH Activity in Rat Embryos

Diabetes ◽  
2003 ◽  
Vol 52 (5) ◽  
pp. 1222-1228 ◽  
Author(s):  
P. Wentzel ◽  
A. Ejdesjo ◽  
U. J. Eriksson
Keyword(s):  
High Glucose ◽  
Maternal Diabetes ◽  
Rat Embryos ◽  
Gapdh Activity
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