Synthesis and Secretion of the Enamel Matrix Precursor by the Kitten Secretory Ameloblast

1984 ◽  
Vol 120 (3) ◽  
pp. 98-102 ◽  
Author(s):  
Takahísa Sasaki
Keyword(s):  
Enamel Matrix ◽  
Secretory Ameloblast ◽  
Matrix Precursor

scholarly journals Synthesis, Secretion, Degradation, and Fate of Ameloblastin During the Matrix Formation Stage of the Rat Incisor as Shown by Immunocytochemistry and Immunochemistry Using Region-specific Antibodies

1997 ◽  
Vol 45 (10) ◽  
pp. 1329-1340 ◽  
Author(s):  
Takashi Uchida ◽  
Chikage Murakami ◽  
Kazuyoshi Wakida ◽  
Takahiro Satoda ◽  
Naofumi Dohi ◽  
...  
Keyword(s):  
Golgi Apparatus ◽  
Matrix Protein ◽  
Core Protein ◽  
Secretory Granules ◽  
Protein Band ◽  
Enamel Matrix ◽  
Rat Incisor ◽  
Formation Stage ◽  
The Matrix ◽  
Secretory Ameloblast

Rat ameloblastin is a recently cloned tooth-specific enamel matrix protein containing 422 amino acid residues. We investigated the expression of this protein during the matrix formation stage of the rat incisor immunohistochemically and immunochemically, using anti-synthetic peptide antibodies that recognize residues 27–47 (Nt), 98–107 (M-1), 224–232 (M-2), 386–399 (M-3), and 406–419 (Ct) of ameloblastin. Immunohistochemical preparations using antibodies Nt and M-1 stained the Golgi apparatus and secretory granules of the secretory ameloblast and the entire thickness of the enamel matrix. Only M-1 intensely stained the peripheral region of the enamel rods. Immunostained protein bands were observed near 65, 55, and below 22 kD. Immunohistochemical preparations using antibodies M-2 and Ct stained the Golgi apparatus and secretory granules of the ameloblast and the immature enamel adjacent to the secretion sites, but not deeper enamel layers. Immunostaining using M-2 and Ct revealed protein bands near 65 and 40–56 kD, and 65, 55, 48, 36, and 25 kD, respectively. M-3 stained the cis side of the Golgi apparatus but not the enamel matrix. This antibody recognized a protein band near 55 kD, but none larger. After brefeldin A treatment, immunoreaction of the 55-kD protein band intensified, and dilated cisternae of rER of the secretory ameloblast contained immunoreactive material irrespective of the antibodies used. These data indicate that ameloblastin is synthesized as a 55-kD core protein and then is post-translationally modified with O-linked oligosaccharides to become the 65-kD secretory form. Initial cleavages of the 65-kD protein generate N-terminal polypeptides, some of which concentrate in the prism sheath, and C-terminal polypeptides, which are rapidly degraded and lost from the enamel matrix soon after secretion.

Autoradiography of Incorporated Leucine-H3 in the Enamel Matrix and Enamel Organ of the Upper Incisor of the Rat

1964 ◽  
Vol 04 (02) ◽  
pp. 186-192
Author(s):  
Leonel Costacurta
Keyword(s):  
Amino Acid ◽  
Blood Vessels ◽  
Tooth Germ ◽  
Longitudinal Section ◽  
Equal Length ◽  
Enamel Organ ◽  
Enamel Matrix ◽  
Distal Extremity ◽  
Old Rats ◽  
Thin Band

SummaryDental germs of the upper incisors of six-days old rats were studied for the uptake of leucine-H3 by different layers of the enamel organ in correlation to the various stages of the development of enamel.The longitudinal section of the tooth germ was divided into 15 zones of about equal length in order to facilitate the description and interpretation of results. Autoradiographic images of the histologic preparations from rats sacrificed 30 minutes, 1 hour, 1 day and 3 days after the injection were made. The strongest reactions were observed in dental germs of rats sacrificed 1 hour, and particularly one day, after the leucine-H3 injection.The uptake of this compound by the enamel matrix increases progressively up to the young enamel and then decreases to the distal extremity; the greatest quantity of this labeled amino-acid was observed in the primary and young enamel. The reactions were present in the transitional enamel only along a thin band close to the dentine-enamel junction.In the enamel organ leucine-H3 incorporation was greatest in the three layers, the zones corresponding to primary and young enamel. In zones corresponding to transitional enamel, the inner epithelium showed a small quantity, and the stellate reticulum a blackening only in its superficial part, were the blood vessels reach the enamel organ.

Enamel Matrix Derivative: An effective regenerative modality (A Literature Review)

2012 ◽  
Vol 2 (7) ◽  
pp. 323-325
Author(s):  
Dr. Ranjit Singh Uppal ◽  
◽  
Dr. Atamjit Singh Dr. Atamjit Singh ◽  
Dr. Rajbir Kaur ◽  
Dr. Navdeep Kaur Sodhi
Keyword(s):  
Literature Review ◽  
Enamel Matrix Derivative ◽  
Enamel Matrix ◽  
Matrix Derivative

Microstructural control of pitch matrix carbon-carbon composite by iodine treatment

1998 ◽  
Vol 13 (2) ◽  
pp. 302-307 ◽  
Author(s):  
H. Kajiura ◽  
Y. Tanabe ◽  
E. Yasuda ◽  
A. Kaiho ◽  
I. Shiota ◽  
...  
Keyword(s):  
Coal Tar ◽  
Carbon Composite ◽  
Coal Tar Pitch ◽  
Microstructural Development ◽  
Carbon Yield ◽  
Graphitic Structure ◽  
Matrix Microstructure ◽  
Iodine Treatment ◽  
Matrix Precursor ◽  
Microstructural Control

Matrix microstructure of a pitch-based carbon-carbon composite was controlled by an iodine treatment. Coal-tar pitch having the softening point of 101 °C was used as a matrix precursor. The iodine treatment was carried out on a pitch-impregnated specimen at 90 °C for 3–20 h. The specimen was carbonized at 800 °C and graphitized at 2000–3000 °C. The carbon yield increased from 73% to 93% by the iodine treatment. Microstructures of carbonized specimens changed from a flow type texture to a mosaic type one by the iodine treatment. The microstructural development to graphitic structure was suppressed by the iodine treatment.

The effect of enamel matrix protein on gingival tissue thickness in vivo

Odontology ◽  
2011 ◽  
Vol 100 (1) ◽  
pp. 61-66 ◽  
Author(s):  
Khalid Al-Hezaimi ◽  
Hamad Al-Fahad ◽  
Rory O’Neill ◽  
Levi Shuman ◽  
Terrence Griffin
Keyword(s):  
Matrix Protein ◽  
Gingival Tissue ◽  
Enamel Matrix ◽  
Tissue Thickness ◽  
Enamel Matrix Protein

scholarly journals Flapless application of enamel matrix derivative in periodontal retreatment: A multicenter randomized feasibility trial

2021 ◽  
Author(s):  
Holger F.R. Jentsch ◽  
Mario Roccuzzo ◽  
Andrea Pilloni ◽  
Adrian Kasaj ◽  
Rolf Fimmers ◽  
...  
Keyword(s):  
Feasibility Trial ◽  
Enamel Matrix Derivative ◽  
Enamel Matrix ◽  
Matrix Derivative
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