Effects of Retinoic Acid on the Development of the Facial Skeleton in Hamsters: Early Changes Involving Cranial Neural Crest Cells

1983 ◽  
Vol 116 (2) ◽  
pp. 180-192 ◽  
Author(s):  
M.J. Wiley ◽  
P. Cauwenbergs ◽  
I.M. Taylor
Keyword(s):  
Retinoic Acid ◽  
Neural Crest ◽  
Neural Crest Cells ◽  
Cranial Neural Crest ◽  
Facial Skeleton ◽  
Cranial Neural Crest Cells

Retinoic Acid Deficiency Underlies the Etiology of Midfacial Defects

2022 ◽  
pp. 002203452110620
Author(s):  
Y. Wu ◽  
H. Kurosaka ◽  
Q. Wang ◽  
T. Inubushi ◽  
K. Nakatsugawa ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Neural Crest ◽  
Substantial Reduction ◽  
Neural Crest Cells ◽  
Early Embryogenesis ◽  
Cranial Neural Crest ◽  
Loss Of Function ◽  
Shh Signaling ◽  
Cellular Events ◽  
Cranial Neural Crest Cells

Embryonic craniofacial development depends on the coordinated outgrowth and fusion of multiple facial primordia, which are populated with cranial neural crest cells and covered by the facial ectoderm. Any disturbance in these developmental events, their progenitor tissues, or signaling pathways can result in craniofacial deformities such as orofacial clefts, which are among the most common birth defects in humans. In the present study, we show that Rdh10 loss of function leads to a substantial reduction in retinoic acid (RA) signaling in the developing frontonasal process during early embryogenesis, which results in a variety of craniofacial anomalies, including midfacial cleft and ectopic chondrogenic nodules. Elevated apoptosis and perturbed cell proliferation in postmigratory cranial neural crest cells and a substantial reduction in Alx1 and Alx3 transcription in the developing frontonasal process were associated with midfacial cleft in Rdh10-deficient mice. More important, expanded Shh signaling in the ventral forebrain, as well as partial abrogation of midfacial defects in Rdh10 mutants via inhibition of Hh signaling, indicates that misregulation of Shh signaling underlies the pathogenesis of reduced RA signaling-associated midfacial defects. Taken together, these data illustrate the precise spatiotemporal function of Rdh10 and RA signaling during early embryogenesis and their importance in orchestrating molecular and cellular events essential for normal midfacial development.

scholarly journals A novel spalt gene expressed in branchial arches affects the ability of cranial neural crest cells to populate sensory ganglia

2004 ◽  
Vol 1 (1) ◽  
pp. 57-63 ◽  
Author(s):  
MEYER BAREMBAUM ◽  
MARIANNE BRONNER-FRASER
Keyword(s):  
Neural Crest ◽  
Trigeminal Ganglion ◽  
Neural Tube ◽  
Cell Lineage ◽  
Neural Crest Cells ◽  
Cranial Neural Crest ◽  
Facial Skeleton ◽  
Differentiation Markers ◽  
Branchial Arches ◽  
Cranial Neural Crest Cells

Cranial neural crest cells differentiate into diverse derivatives including neurons and glia of the cranial ganglia, and cartilage and bone of the facial skeleton. Here, we explore the function of a novel transcription factor of the spalt family that might be involved in early cell-lineage decisions of the avian neural crest. The chicken spalt4 gene (csal4) is expressed in the neural tube, migrating neural crest, branchial arches and, transiently, in the cranial ectoderm. Later, it is expressed in the mesectodermal, but not neuronal or glial, derivatives of midbrain and hindbrain neural crest. After over-expression by electroporation into the cranial neural tube and neural crest, we observed a marked redistribution of electroporated neural crest cells in the vicinity of the trigeminal ganglion. In control-electroporated embryos, numerous, labeled neural crest cells (∼80% of the population) entered the ganglion, many of which differentiated into neurons. By contrast, few (∼30% of the population) spalt-electroporated neural crest cells entered the trigeminal ganglion. Instead, they localized in the mesenchyme around the ganglionic periphery or continued further ventrally to the branchial arches. Interestingly, little or no expression of differentiation markers for neurons or other cell types was observed in spalt-electroporated neural crest cells.

Laser capture microdissection of fluorescently labeled embryonic cranial neural crest cells

genesis ◽  
2004 ◽  
Vol 39 (1) ◽  
pp. 58-64 ◽  
Author(s):  
Vasker Bhattacherjee ◽  
Partha Mukhopadhyay ◽  
Saurabh Singh ◽  
Emily A. Roberts ◽  
Rita C. Hackmiller ◽  
...  
Keyword(s):  
Neural Crest ◽  
Laser Capture Microdissection ◽  
Neural Crest Cells ◽  
Cranial Neural Crest ◽  
Cranial Neural Crest Cells ◽  
Laser Capture
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