Comparative histochemical study of phosphorylase activity in the mammalian testis

1974 ◽  
Vol 90 (4) ◽  
pp. 573-584
Author(s):  
Carlos Ohanian ◽  
Mario Micucci ◽  
Heriberto Rodríguez
Keyword(s):  
Histochemical Study ◽  
Phosphorylase Activity ◽  
Mammalian Testis

Histochemical Study of Glycogen and Phosphorylase Activity on Bilateral Biopsies of Oligospermic Men with Varicocele

1983 ◽  
Vol 10 (1) ◽  
pp. 79-83 ◽  
Author(s):  
M. Re ◽  
M. Iannitelli ◽  
A. Cerasaro ◽  
L. Santoro ◽  
M. Cuomo ◽  
...  
Keyword(s):  
Histochemical Study ◽  
Phosphorylase Activity

scholarly journals A QUANTITATIVE AND HISTOCHEMICAL STUDY OF PHOSPHORYLASE IN THE PLACENTA

1966 ◽  
Vol 14 (8) ◽  
pp. 582-589 ◽  
Author(s):  
T. ADESANYA IGE GRILLO
Keyword(s):  
Histochemical Study ◽  
Fetal Liver ◽  
Active Form ◽  
Phosphorylase Activity ◽  
Decidua Basalis ◽  
Weak Activity ◽  
Chorioallantoic Placenta ◽  
Visceral Layer ◽  
Cytotrophoblastic Cells ◽  
Made In

A quantitative and histochemical study of phosphorylase has been made in the human, rat and rabbit placentae. The placental enzyme was found to have the same optimal pH as liver phosphorylase. Since cyclic 3',5'-AMP, glucagon or adrenaline had no influence on enzyme activity, phosphorylase probably exists only in the active form in the placenta. The activity of phosphorylase was localized histochemically in the decidua basalis, the cytotrophoblast of the spongy zone of the chorioallantoic placenta and in the visceral layer of the inverted yolk sac of the rat. It was present mainly in the decidua basalis of placenta of the rabbit although a few cytotrophoblastic cells of the trophoblastic tubules also showed weak activity. In the human placenta the enzyme was active in the cytotrophoblast and the mesodermal core of the villi. It was present occasionally in the syncytiotrophoblast. The quantity of the enzyme fluctuates during gestation in both the human and rat fetal placentae. These fluctuations do not appear to bear relation to either placental glycogen level or to fetal liver phosphorylase activity. Nor is there any obvious correlation between placental phosphorylase and the activity of glucagon-like substance of the fetal pancreas.

scholarly journals Eosinophilic Leukemia A Morphologic and Histochemical Study

Blood ◽  
1964 ◽  
Vol 24 (4) ◽  
pp. 372-388 ◽  
Author(s):  
G. ADOLPH ACKERMAN
Keyword(s):  
Amino Acids ◽  
Cell Size ◽  
Enzyme Activities ◽  
Histochemical Study ◽  
Leukemic Cells ◽  
Phosphorylase Activity ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Cytoplasmic Maturation ◽  
Histochemical Reactivity

Abstract Correlated histochemical, phase and electron microscopic studies were employed in examining the eosinophils from a patient with acute eosinophilic leukemia. Numerous morphologic alterations were observed in the leukemic eosinophils and eosinophilic myelocytes. These alterations included asynchronous nuclear-cytoplasmic maturation; an increase in cell size; the formation of eosinophilic granules which vary markedly in number, size, contour, and density; and the presence of fibrillar formations in some of the leukemic cells. Histochemically, the major alterations observed in the leukemic cells were the extensive deposition of glycogen in the cytoplasm and the demonstration of increased phosphorylase activity in these cells. Other minor variations in the histochemical reactivity of the leukemic eosinophils also have been described. Histochemical procedures included technics for proteins, amino acids, carbohydrates, hydrolytic and oxidative enzyme activities.

5-NUCLEOTIDASE, ACID PHOSPHATASE AND PHOSPHORYLASE DURING NORMAL, DELAYED AND INDUCED IMPLANTATION OF BLASTOCYSTS IN MICE: A HISTOCHEMICAL STUDY

1971 ◽  
Vol 51 (2) ◽  
pp. 291-301 ◽  
Author(s):  
KATHLEEN HALL
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Stromal Cells ◽  
Histochemical Study ◽  
Muscle Fibres ◽  
Nucleotidase Activity ◽  
Phosphorylase Activity ◽  
Decidual Cells ◽  
Glycogen Deposition ◽  
Parallel Distribution

SUMMARY The distribution of histochemically demonstrable 5′-nucleotidase activity in the uterus of mice around the time of nidation, is compared with those of specific and non-specific acid phosphatases, alkaline phosphatase, phosphorylase, and with deposition of glycogen. The sequences of changes in distribution were similar during normal (first pregnancy) or delayed (by lactation) implantation, or when implantation was induced in lactating mice by administration of hormones. 5′-Nucleotidase activity was located at the free border of the luminal epithelium and at surfaces of stromal cells and muscle fibres up to the time of implantation, and in non-decidualized areas after implantation, but disappeared from differentiated decidual cells. The disappearance of 5′-nucleotidase activity from decidual cells occurred at the time when their mitotic activity was waning and when alkaline phosphatase activity had appeared at their surfaces, and lagged slightly behind both glycogen deposition and increased phosphorylase activity in these cells. There was parallel distribution of phosphorylase activity and glycogen distribution within decidua. At the time of implantation, a reaction appeared in stromal cells in the immediate vicinity of the blastocyst after incubation with either adenosine-5′-monophosphate or glycerophosphate at acid pH, and it spread in area during the next 24 h. It was believed to indicate activity of a non-specific acid hydrolase, possibly lysosomal. In all uteri examined, epithelial cells of glands showed specific acid phosphatase, but no 5′-nucleotidase activity.

Phosphorylase activity in the normal human testis: A histochemical study

1972 ◽  
Vol 28 (9) ◽  
pp. 1106-1107 ◽  
Author(s):  
M. Re. V. Santiemma ◽  
G. Frajese ◽  
M. Savioli ◽  
A. Fabbrini
Keyword(s):  
Histochemical Study ◽  
Human Testis ◽  
Phosphorylase Activity ◽  
Normal Human

A Clinical and Histochemical Study of Celiac Disease Before and During a Gluten-Free Diet

1965 ◽  
Vol 48 (2) ◽  
pp. 155-172 ◽  
Author(s):  
I. Michael Samloff ◽  
John S. Davis ◽  
Eric A. Schenk
Keyword(s):  
Celiac Disease ◽  
Histochemical Study ◽  
Gluten Free Diet ◽  
Gluten Free
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