Histochemical reactions on the localization of lipids and carbohydrates in the diencephalon and the mesencephalon of Barilius bendelisis (Ham.) and Barilius vagra(Ham.)

1974 ◽  
Vol 89 (4) ◽  
pp. 560-571
Author(s):  
K.K. Tandon ◽  
I.K. Sukheja
Keyword(s):  
2021 ◽  
Vol 79 (1) ◽  
pp. 15-24
Author(s):  
Murali Sanjeev Kumar ◽  
Pankaj Soni ◽  
Ravindra Kumar ◽  
Neha Singh ◽  
Shreya Srivastava ◽  
...  

Abstract A cell line named BBdF-1, established from the caudal fin of hill stream fish Barilius bendelisis, has been subcultured for more than 52 passages and is being maintained in L-15 media containing 20% FBS. Species origin of the cell line was confirmed using amplification of partial region of 16S and COI mitochondrial gene sequences. The optimum temperature for growth of BBdF-1 cell line was found to be 28°C. Karyotyping revealed diploid chromosome number as 50. Cells exhibited strong binding for cytokeratin marker and thus were found to be epithelial-like. Strong green fluorescence was observed in BBdF-1 cells transfected with phrGFP-II-N vector, indicating its suitability for utilization in gene expression and manipulation studies. Successful assessment of cytotoxicity of two heavy metals, viz. mercury and chromium, was performed. The cell line can serve as a useful resource material for early toxicity screening of pesticides/pollutant and gene expression.


2018 ◽  
Vol 27 (4) ◽  
pp. 975-988
Author(s):  
Neeraj Kumar Sharma ◽  
M. S. Akhtar ◽  
Ravindra Singh ◽  
N. N. Pandey

2019 ◽  
Vol 35 (6) ◽  
pp. 1242-1248
Author(s):  
Ansuman Panda ◽  
Debashis Jena ◽  
Mrinal Kanti Datta ◽  
Janmejay Parhi ◽  
Radhakrishnan Kizhakke Veettil ◽  
...  

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