A histocytological investigation on epithelioneural associations in the lesser omentum of the eleven day chick embryo (Gallus domesticus), with special reference to the application of van Campenhout’s ammoniated silver carbonate technique

1970 ◽  
Vol 76 (3) ◽  
pp. 409-424
Author(s):  
Maria V. Dreyer
Author(s):  
C.D. Fermin ◽  
M. Igarashi

Otoconia are microscopic geometric structures that cover the sensory epithelia of the utricle and saccule (gravitational receptors) of mammals, and the lagena macula of birds. The importance of otoconia for maintanance of the body balance is evidenced by the abnormal behavior of species with genetic defects of otolith. Although a few reports have dealt with otoconia formation, some basic questions remain unanswered. The chick embryo is desirable for studying otoconial formation because its inner ear structures are easily accessible, and its gestational period is short (21 days of incubation).The results described here are part of an intensive study intended to examine the morphogenesis of the otoconia in the chick embryo (Gallus- domesticus) inner ear. We used chick embryos from the 4th day of incubation until hatching, and examined the specimens with light (LM) and transmission electron microscopy (TEM). The embryos were decapitated, and fixed by immersion with 3% cold glutaraldehyde. The ears and their parts were dissected out under the microscope; no decalcification was used. For LM, the ears were embedded in JB-4 plastic, cut serially at 5 micra and stained with 0.2% toluidine blue and 0.1% basic fuchsin in 25% alcohol.


Development ◽  
1962 ◽  
Vol 10 (3) ◽  
pp. 423-450
Author(s):  
P. H. S. Silver

The present communication concerns the influence in the chick (Gallus domesticus) of the growing eyes on various structures which form the wall of the orbits, or which lie in their vicinity. Features of the development of the trabeculae cranii, the fronto-nasal process, the nasal capsules, the maxillary ‘process’, the otic capsule, and the cephalic flexure will be described as well as certain growth relationships of the upper and lower jaws. The methods employed include (1) removal of one or both primary optic vesicles, (2) the grafting of an additional primary vesicle into the head mesenchyme so that two eyes develop in one orbit, and (3) the isolation in the coelom of various head components. The importance of the trabeculae to our present study stems from the fact that they contribute to the boundaries of the orbits on their medial sides.


1988 ◽  
Vol 182 (2) ◽  
pp. 169-182 ◽  
Author(s):  
Shigeru Kuratani ◽  
Shigenori Tanaka ◽  
Yuji Ishikawa ◽  
Chosei Zukeran

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