The Role of the TGF/Smad Signaling Pathway in Peritoneal Fibrosis Induced by Peritoneal Dialysis Solutions

2008 ◽  
Vol 109 (2) ◽  
pp. e71-e78 ◽  
Author(s):  
Qiang Yao ◽  
Krzysztof Pawlaczyk ◽  
Ernesto Rodríguez Ayala ◽  
Arkadiusz Styszynski ◽  
Andrzej Breborowicz ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Signaling Pathway ◽  
Peritoneal Fibrosis ◽  
Smad Signaling ◽  
Smad Signaling Pathway ◽  
Dialysis Solutions ◽  
Peritoneal Dialysis Solutions

scholarly journals Role of Tenascin-X in regulating TGF-β/Smad signaling pathway in pathogenesis of slow transit constipation

2020 ◽  
Vol 26 (7) ◽  
pp. 717-724
Author(s):  
Yi-Chao Zhang ◽  
Bao-Xiang Chen ◽  
Xiao-Yu Xie ◽  
Yan Zhou ◽  
Qun Qian ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Slow Transit Constipation ◽  
Smad Signaling ◽  
Smad Signaling Pathway ◽  
Slow Transit ◽  
Transit Constipation

New Animal Models for Encapsulating Peritoneal Sclerosis—Role of Acidic Solution

2001 ◽  
Vol 21 (3_suppl) ◽  
pp. 349-353 ◽  
Author(s):  
Hidetomo Nakamoto ◽  
Hiroe Imai ◽  
Yuji Ishida ◽  
Yasuhiro Yamanouchi ◽  
Tsutomu Inoue ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Peritoneal Fibrosis ◽  
Solution Ph ◽  
Group Iii ◽  
Group I ◽  
Dialysis Solution ◽  
Wistar Kyoto ◽  
Dialysis Solutions

Objective Encapsulating peritoneal sclerosis (EPS), in which all or part of the intestine is enveloped in a fibrous ball resembling a cocoon, is a serious complication of peritoneal dialysis (PD). The aim of the present study was to investigate whether pH-neutral or acidic dialysis solutions induce peritoneal fibrosis. Design We divided 18 male Wistar–Kyoto (WKY) rats into three groups and dialyzed them with various solutions as follows: group I, 10 mL acidic dialysis solution (pH 3.8, containing 1.35% glucose), n = 6; group II, 10 mL pH 5.0 dialysis solution, n = 6; and group III, 10 mL neutral dialysis solution (pH 7.0), n = 6. Peritoneal catheters were inserted, and dialysis solution was injected every day for 40 days. At the end of the experiment, a peritoneal equilibration test (PET) was performed. Expression of mRNA of aquaporins 1 and 4 (AQP-1 and AQP-4) in the peritoneum were studied by semiquantitative reverse-transcriptase polymerase chain reaction (RT-PCR). Results In rats treated with pH 3.8 dialysis solution, necropsy findings revealed features identical to those of EPS. The typical appearance was of granulation tissue or fibrotic tissue (or both) covering multiple surfaces. Multiple adhesions were present. In microscopic examinations, peritoneal fibrosis and loss of mesothelium were found. In rats treated with pH 7.0 dialysis solution, no signs of EPS were seen. In rats treated with pH 5.0 dialysis solution, milder changes (subserosal thickening and partial adhesion of the peritonea) were observed. The mRNA of AQP-1 and AQP-4 were expressed in the peritonea of the rats. The expression of the AQPs was significantly suppressed in rats treated with pH 3.8 dialysis solution. Conclusions In rats, long-term intraperitoneal injection of acidic dialysis solution produced features typical of EPS in humans. Newly developed neutral dialysis solutions protected the against the development of EPS during peritoneal dialysis in rats.

scholarly journals Role of pioglitazone on gene/protein expression profile, bioenergetics and TGFβ/SMAD signaling pathway in NSCLC

2018 ◽  
Vol 29 ◽  
pp. viii678
Author(s):  
R. Di Liello ◽  
G. Viscardi ◽  
V. Ciaramella ◽  
G. Barra ◽  
G. Esposito ◽  
...  
Keyword(s):  
Protein Expression ◽  
Signaling Pathway ◽  
Expression Profile ◽  
Protein Expression Profile ◽  
Smad Signaling ◽  
Smad Signaling Pathway

MiR-655-3p inhibits the progression of osteoporosis by targeting LSD1 and activating BMP-2/Smad signaling pathway

2020 ◽  
Vol 39 (10) ◽  
pp. 1390-1404
Author(s):  
X-J Wang ◽  
J-W Liu ◽  
J Liu
Keyword(s):  
Osteogenic Differentiation ◽  
Signaling Pathway ◽  
Underlying Mechanism ◽  
Bone Diseases ◽  
Bone Morphogenetic Protein 2 ◽  
Metabolic Bone Diseases ◽  
Smad Signaling ◽  
Smad Signaling Pathway

Osteoporosis (OP) is one of the most common chronic metabolic bone diseases in the seniors and postmenopausal women. Plenty of microRNAs (miRNAs) have been confirmed to be involved in OP progression. However, the role of miR-655-3p in osteogenic differentiation and bone formation was still unclear. In this study, we aimed to investigate the cellular function of miR-655-3p and its underlying mechanism in OP. We found that miR-655-3p expression was downregulated in both ovariectomized (OVX) mice bone tissues and MC3T3-E1 cells treated with simulated microgravity (MG). MiR-655-3p overexpression facilitated cell differentiation but suppressed cell apoptosis of MC3T3-E1 cells induced by simulated MG. Mechanistically, we confirmed that lysine-specific histone demethylase 1 (LSD1) is a downstream target gene of miR-655-3p. Furthermore, overexpression of miR-655-3p activated the bone morphogenetic protein 2 (BMP-2)/decapentaplegic homolog (Smad) signaling pathway by suppressing LSD1 expression. Moreover, LSD1 knockdown accelerated osteogenic differentiation and inhibited apoptosis in MC3T3-E1 cells under simulated MG. Additionally, the OVX mouse model was established to investigate the role of miR-655-3p/LSD1 axis in vivo. The results demonstrated that LSD1 could reverse the effects triggered by the injection of adeno-associated virus-miR-655-3p on OP development. Further investigations revealed that miR-655-3p boosted osteogenic differentiation through LSD1/BMP-2/Smad signaling pathway. In summary, these findings implied a potential value of miR-655-3p in OP therapy.

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