ON THE HISTOCHEMICAL DEMONSTRATION OF ADRENERGIC NERVES WITH THE OSMIC ACID-SODIUM IODIDE TECHNIQUE

1959 ◽  
Vol 38 (4) ◽  
pp. 379-384 ◽  
Author(s):  
Nils-Åke Hillarp
Keyword(s):  
Sodium Iodide ◽  
Histochemical Demonstration ◽  
Adrenergic Nerves ◽  
Osmic Acid

scholarly journals MICROFLUORIMETRIC STUDIES ON THE FORMALDEHYDE-INDUCED FLUORESCENCE OF NORADRENALINE IN ADRENERGIC NERVES OF RAT IRIS

1969 ◽  
Vol 17 (11) ◽  
pp. 714-723 ◽  
Author(s):  
GÖSTA JONSSON
Keyword(s):  
Fluorescence Intensity ◽  
Linear Part ◽  
Fluorescence Yield ◽  
Histochemical Demonstration ◽  
Adrenergic Nerves ◽  
Noradrenaline Concentration ◽  
Amine Storage ◽  
Rat Iris ◽  
Gaseous Formaldehyde

The fluorescence concentration relationship of formaldehyde-induced fluorescence of noradrenaline in adrenergic nerves of rat iris was investigated by the use of isotope and microfluorimetric techniques. The irides first were depleted of their endogenous stores of noradrenaline either by reserpine or by the methylester of α-methyl- p-tyrosine (H 44/68) and then were incubated in vitro in a physiologic medium containing 3H-noradrenaline for partial replenishment of the nerves of their transmitter. The incubated irides were prepared as whole amounts and were exposed to gaseous formaldehyde for histochemical demonstration of noradrenaline. Microfluorimetric and isotope measurements were performed on the same preparations. The fluorescence intensity was found to be proportional to the noradrenaline concentration up to a value corresponding to 30-40% of the endogenous level, above which a concentration-dependent quenching of the fluorescence occurred. In the linear part of the relation, it was possible to perform fairly safe estimations of the fluorescence intensity by eye, but, if the nerves contained more than 40% of the endogenous content, usually no differentiation could be made, probably as a result of quenching effects. The quenching appears somewhat earlier and the relative fluorescence yield is lower when noradrenaline is stored in the intraneuronal amine storage granules, compared with the situation when the amine mainly is distributed extragranularly in the axoplasm. It can be concluded that, if changes in fluorescence intensity as compared with the control are observed, this reflects true changes in amine concentrations, but changes in amine concentration may escape detection when the concentration of amine is so high that quenching occurs.

Histochemical Demonstration of Adrenergic Nerves in Cortex-Pia of Rabbit

2009 ◽  
Vol 23 (2-3) ◽  
pp. 133-142 ◽  
Author(s):  
B. Falck ◽  
G. I. Mchedlishvili ◽  
Ch. Owman
Keyword(s):  
Histochemical Demonstration ◽  
Adrenergic Nerves

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

The diagnosis of metabolic storage disease in skin biopsies (a light-, electronmicroscopic, and analytical study)

1978 ◽  
Vol 36 (2) ◽  
pp. 648-649
Author(s):  
W. Jurecka ◽  
W. Gebhart ◽  
H. Lassmann
Keyword(s):  
Storage Disease ◽  
Hunter Syndrome ◽  
Histochemical Demonstration ◽  
Sweat Glands ◽  
Type I ◽  
Storage Material ◽  
Scheie Syndrome ◽  
Storage Products ◽  
Skin Biopsies ◽  
Type V

Diagnosis of metabolic storage disease can be established by the determination of enzymes or storage material in blood, urine, or several tissues or by clinical parameters. Identification of the accumulated storage products is possible by biochemical analysis of isolated material, by histochemical demonstration in sections, or by ultrastructural demonstration of typical inclusion bodies. In order to determine the significance of such inclusions in human skin biopsies several types of metabolic storage disease were investigated. The following results were obtained.In MPS type I (Pfaundler-Hurler-Syndrome), type II (Hunter-Syndrome), and type V (Ullrich-Scheie-Syndrome) mainly “empty” vacuoles were found in skin fibroblasts, in Schwann cells, keratinocytes and macrophages (Dorfmann and Matalon 1972). In addition, prominent vacuolisation was found in eccrine sweat glands. The storage material could be preserved in part by fixation with cetylpyridiniumchloride and was also present within fibroblasts grown in tissue culture.

Comparative Study of the Fine Morphology of Sensory Receptors in Aspidogaster conchicola and Cotylaspis insignis

1972 ◽  
Vol 30 ◽  
pp. 150-151
Author(s):  
Venita F. Allison ◽  
J. E. Ubelaker ◽  
J. H. Martin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Nervous System ◽  
Osmic Acid ◽  
Sensory Receptors ◽  
Transmission Electron ◽  
Sensory Structures ◽  
Fine Morphology ◽  
Scanning Electron

It has been suggested that parasitism results in a reduction of sensory structures which concomitantly reflects a reduction in the complexity of the nervous system. The present study tests this hypothesis by examining the fine morphology and the distribution of sensory receptors for two species of aspidogastrid trematodes by transmission and scanning electron microscopy. The species chosen are an ectoparasite, Cotylaspis insignis and an endoparasite, Aspidogaster conchicola.Aspidogaster conchicola and Cotylaspis insignis were obtained from natural infections of clams, Anodonta corpulenta and Proptera purpurata. The specimens were fixed for transmission electron microscopy in phosphate buffered paraformaldehyde followed by osmic acid in the same buffer, dehydrated in an ascending series of ethanol solutions and embedded in Epon 812.

Morphological Observations on the Granule Types in Rabbit Extra-Adrenal Chromaffin Cells.

1975 ◽  
Vol 33 ◽  
pp. 318-319
Author(s):  
Joe A. Mascorro ◽  
Robert D. Yates
Keyword(s):  
Chromaffin Cells ◽  
Sodium Phosphate ◽  
Ganglion Cells ◽  
Ultrastructural Level ◽  
Osmic Acid ◽  
Adrenal Chromaffin Cells ◽  
Potassium Iodate ◽  
Perfusion Fluid ◽  
New Zealand White Rabbits ◽  
Adrenal Chromaffin

Extra-adrenal chromaffin organs (abdominal paraganglia) constitute rich sources of catecholamines. It is believed that these bodies contain norepinephrine exclusively. However, the present workers recently observed epinephrine type granules in para- ganglion cells. This report investigates catecholamine containing granules in rabbit paraganglia at the ultrastructural level.New Zealand white rabbits (150-170 grams) were anesthetized with 50 mg/kg Nembutal (IP) and perfused with 3% glutaraldehyde buffered with 0.2M sodium phosphate, pH 7.3. The retroperitoneal tissue blocks were removed and placed in perfusion fluid for 4 hours. The abdominal paraganglia were dissected from the blocks, diced, washed in phosphate buffer and fixed in 1% osmic acid buffered with phosphate. In other animals, the glutaraldehyde perfused tissue blocks were immersed for 1 hour in 3% glutaraldehyde/2.5% potassium iodate buffered as before. The paraganglia were then diced, separated into two vials and washed in the buffer. A portion of this tissue received osmic acid fixation.

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