In vitro Effect of Amikacin on Rat and Human Detrusor Muscle Contraction

2008 ◽  
Vol 81 (1) ◽  
pp. 94-100
Author(s):  
M. Gardi ◽  
F. Nigro ◽  
E. Ragazzi ◽  
A. Volpe ◽  
A. Totaro ◽  
...  
Keyword(s):  
Muscle Contraction ◽  
Detrusor Muscle ◽  
Vitro Effect ◽  
Human Detrusor

Inhibition of isolated rat and human detrusor muscle contraction by disopyramide

2000 ◽  
Vol 20 (4) ◽  
pp. 229-236
Author(s):  
R. A. Elliott ◽  
D. Hudman ◽  
T. Terry ◽  
D. Sandhu ◽  
R. I. Norman
Keyword(s):  
Muscle Contraction ◽  
Detrusor Muscle ◽  
Human Detrusor ◽  
Isolated Rat

Effect of Treatment with Calcium Antagonists in Vitro and in Vivo on the Contractile Response of Isolated Rat and Human Detrusor Muscle

1996 ◽  
Vol 91 (4) ◽  
pp. 467-474 ◽  
Author(s):  
Ruth A. Elliott ◽  
Robert I. Norman ◽  
Stuart G. Parker ◽  
R. Paul Whitaker ◽  
C. Mark Castleden
Keyword(s):  
Single Dose ◽  
Calcium Antagonists ◽  
Contractile Response ◽  
Detrusor Muscle ◽  
Significant Difference ◽  
Effect Of Treatment ◽  
Human Detrusor ◽  
Isolated Rat

1. The effect of calcium antagonists on the contractile response of human and rat isolated detrusor muscle in vitro was investigated. The effect of treatment with nimodipine on rat detrusor muscle in vivo was also examined. 2. Nimodipine 0.1 μmol/l, nifedipine 0.1 μmol/l, nifedipine 0.25 μmol/l and verapamil 1.5 μmol/l reduced the maximum contractile response of isolated human detrusor muscle to carbachol by 42%, 35%, 41% and 28% respectively (P < 0.01). Verapamil 0.1 μmol/l had no significant effect on contractile response. 3. Nimodipine 0.1 μmol/l reduced the maximum contractile response of isolated rat detrusor muscle in vitro to electrical field stimulation and carbachol by 53% and 84% respectively (P < 0.01). 4. Rats were pretreated with nimodipine for 8 days (5 mg day−1 kg−1) or with a single dose. Serum nimodipine concentrations were higher in rats treated for 8 days. In rats treated with nimodipine for 8 days there was no significant difference in detrusor contractile response compared with controls. However, after one dose of nimodipine the maximum contractile response was significantly reduced compared with controls (P < 0.05). 5. At the concentrations studied, nimodipine had a greater inhibitory effect on the contractile response of isolated human detrusor muscle. Nimodipine significantly reduced the contractile response of rat detrusor muscle in vitro and after a single dose in vivo, but had no significant effect after 8 days' treatment in vivo. It is possible that chronic oral treatment with nimodipine caused an up-regulation of 1,4-dihydropyridine-sensitive calcium channels, which may explain the lack of clinical effect of chronic treatment with calcium antagonists in patients with detrusor instability.

Amikacin: A Novel Modulator of Vesical and Prostate Efferences. An in vitro Experimental Study

2007 ◽  
Vol 74 (4) ◽  
pp. 217-227
Author(s):  
M. Gardi ◽  
F. Nigro ◽  
E. Ragazzi ◽  
A. Volpe ◽  
A. Totaro ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Urinary Tract ◽  
Electrical Field Stimulation ◽  
Smooth Muscle Contraction ◽  
Detrusor Muscle ◽  
Detrusor Smooth Muscle ◽  
Related Compounds ◽  
Human Detrusor ◽  
Isolated Rat

The autonomic efferent neurotransmission to the bladder and prostate smooth muscle is a potential target for drug therapy of specific low urinary tract disfunction (LUTD). Since amikacin and other amynoglicosides were reported to affect neurotransmission by a pre-junctional mechanism, we investigated the effect of amikacin on isolated rat and human detrusor smooth muscle contraction and on isolated rat and human prostate contraction, to further evaluate its potential relaxant properties. Materials and Methods Samples of detrusor smooth muscle and prostate tissue, obtained from 97 rats and 16 patients undergoing surgery, were studied through the measurement of isometric contraction induced by electrical field stimulation (EFS) and other pharmacological stimuli in the presence or absence of 1mM amikacin in a low-Ca medium. Results Amikacin 1 mM significantly reduced contraction of isolated rat and human detrusor muscle and prostate, achieved with pre-junctional stimulation, while no significant effect was observed on contraction induced by pharmacological post-junctional stimulators. EFS contraction inhibited by amikacin was restored after addition of calcium chloride. The amikacin effect was comparable to the effect of magnesium ions, which are known to exert a pre-junctional inhibition of neurotransmitter release. Conclusions Amikacin significantly inhibited rat and human detrusor and prostate contraction evoked by pre-junctional stimulation in vitro, suggesting a depressant effect on autonomic efferent neurotransmission. Further pharmacokinetics studies and researches on related compounds may hold potential for future development in the treatment of specific low urinary tract disfunction (LUTD).

Effects of 138–355, a β3-adrenoceptor selective agonist, on relaxation of the human detrusor muscle in vitro

2006 ◽  
Vol 25 (7) ◽  
pp. 815-819 ◽  
Author(s):  
Tomonori Yamanishi ◽  
Kosaku Yasuda ◽  
Satoshi Kitahara ◽  
Hideo Nakai ◽  
Ken-Ichiro Yoshida ◽  
...  
Keyword(s):  
Detrusor Muscle ◽  
Selective Agonist ◽  
Human Detrusor

The minor population of M3-receptors mediate contraction of human detrusor muscle in vitro

2001 ◽  
Vol 21 (5) ◽  
pp. 243-248 ◽  
Author(s):  
R. Chess-Williams ◽  
C. R. Chapple ◽  
T. Yamanishi ◽  
K. Yasuda ◽  
D. J. Sellers
Keyword(s):  
Detrusor Muscle ◽  
M3 Receptors ◽  
Minor Population ◽  
Human Detrusor

165: β-Adrenoceptor Subtype 1, 2 and 3 Mrna are all Present in Human Detrusor Muscle and Urothelium

2005 ◽  
Vol 173 (4S) ◽  
pp. 45-45 ◽  
Author(s):  
Catherine A. Thomas ◽  
Shachi Tyagi ◽  
Hitoshi Masuda ◽  
Naoki Yoshimura ◽  
Michael B. Chancellor ◽  
...  
Keyword(s):  
Detrusor Muscle ◽  
Human Detrusor
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