Effect of Atropine on Bile Flow and Biliary Excretion of 3H-Ouabain in the Isolated Perfused Rat Liver

Pharmacology ◽  
1977 ◽  
Vol 15 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Kaisu Nevasaari ◽  
Birgitta Alakare ◽  
Niilo T. Kärki
Keyword(s):  
Rat Liver ◽  
Biliary Excretion ◽  
Bile Flow ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver

Malaria infection impairs glucuronidation and biliary excretion by the isolated perfused rat liver

Xenobiotica ◽  
1991 ◽  
Vol 21 (12) ◽  
pp. 1571-1582 ◽  
Author(s):  
R. T. Murdoch ◽  
H. Ghabrial ◽  
G. W. Mihaly ◽  
D. J. Morgan ◽  
R. A. Smallwood
Keyword(s):  
Rat Liver ◽  
Biliary Excretion ◽  
Malaria Infection ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver

Metabolism and biliary excretion of trimetrexate by the isolated perfused rat liver

1985 ◽  
Vol 29 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Lorraine K. Webster ◽  
William P. Tong ◽  
John J. McCormack
Keyword(s):  
Rat Liver ◽  
Biliary Excretion ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver

Biliary excretion of dihydro-11-keto-progesterone-4-C14 by isolated perfused liver

1964 ◽  
Vol 207 (5) ◽  
pp. 1030-1034 ◽  
Author(s):  
G. F. Leong ◽  
D. M. Cazes ◽  
M. L. Berliner ◽  
D. L. Berliner
Keyword(s):  
Rat Liver ◽  
Biliary Excretion ◽  
Half Life ◽  
Water Soluble ◽  
Isolated Perfused Rat Liver ◽  
Perfused Liver ◽  
Rapid Rate ◽  
Perfused Rat Liver ◽  
Bile Formation ◽  
Entire Study

The rates of biliary excretion of dihydro-11-keto-progesterone-4-C14 and of its metabolites were studied in the isolated perfused rat liver. The half-life of this steroid in the perfusing blood was 2.5 min, and at 40 min about 75% of the injected steroid had been excreted in bile. Formation of water-soluble steroids (WS St) took place at a rapid rate and by 60 min 100% of the steroids in blood were found to be water soluble. During the entire study the steroids excreted in bile were water soluble and accounted for 97.2–100% (avg. 98.2%). No dihydro-11-keto-progesterone was found to be excreted in the bile. The rate of disappearance from the blood, excretion in the bile, and degree of formation of WS St of this compound when compared with corticosterone and cortisol shows the following pattern: dihydro-11-keto-progesterone > corticosterone > cortisol.

scholarly journals Cell swelling increases bile flow and taurocholate excretion into bile in isolated perfused rat liver

1992 ◽  
Vol 281 (3) ◽  
pp. 593-595 ◽  
Author(s):  
C Hallbrucker ◽  
F Lang ◽  
W Gerok ◽  
D Häussinger
Keyword(s):  
Bile Acid ◽  
Amino Acid ◽  
Cell Volume ◽  
Rat Liver ◽  
Bile Flow ◽  
Cell Swelling ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Volume Changes ◽  
Close Relationship

The effects of aniso-osmotically and amino-acid-induced cell-volume changes on bile flow and biliary taurocholate excretion were studied in isolated perfused rat liver. With taurocholate (100 microM) in the influent perfusate, hypo-osmotic exposure (225 mosmol/l) increased taurocholate excretion into bile and bile flow by 42 and 27% respectively, whereas inhibition by 32 and 47% respectively was observed after hyperosmotic (385 mosmol/l) exposure. The effects of aniso-moticity on taurocholate excretion into bile was observed throughout aniso-osmotic exposure, even after completion of volume-regulatory ion fluxes and were fully reversible upon re-exposure to normo-osmotic media. Hypo-osmotic cell swelling (225 mosmol/l) increased the Vmax. of taurocholate translocation from the sinusoidal compartment into bile about 2-fold. Also, cell swelling induced by glutamine and glycine stimulated both bile flow and biliary taurocholate excretion. There was a close relationship between the aniso-osmotically and amino-acid-induced change of cell volume and taurocholate excretion into bile. The data suggest that liver cell volume plays an important role in regulating bile-acid-dependent bile flow and biliary taurocholate excretion.

Cation Transport by Slices from the Isolated Perfused Rat Liver

1972 ◽  
Vol 50 (3) ◽  
pp. 276-278
Author(s):  
A. C. Nestruck ◽  
R. W. Furneaux
Keyword(s):  
Rat Liver ◽  
Bile Flow ◽  
Cation Transport ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Bicarbonate Buffer ◽  
Potassium Efflux ◽  
Membrane Function ◽  
Surgical Preparation

Isolated livers from fed rats were perfused for 1 h with a medium consisting of Krebs–Ringer bicarbonate buffer with added albumin and glucose. Rates of perfusate and bile flow, differences in [Formula: see text], [Formula: see text], and pH, and glucose and potassium efflux were measured. Rewarmed slices of liver taken before the surgical preparation and before the perfusion were found to be able to reverse a cation shift imposed by cold incubation. Slices of liver taken after one perfusion were not able to effect this expected cation transport. It is proposed that the perfusate used was not ideal as evidenced by the altered membrane function of slices after perfusion.

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